You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Mouse (Murine) HSPB1 antibody for Western Blotting

Recommended HSPB1 Antibody (supplied by: Log in to see )

Antigen
Heat Shock 27kDa Protein 1 (HSPB1) Antibodies
  • 27kDa
  • 2810021G24Rik
  • cb153
  • cb660
  • cmt2f
  • CMT2F
  • HMN2B
  • hs.76067
  • HS.76067
  • Hs.78846
  • hsp1
  • Hsp25
  • hsp25
  • Hsp27
  • HSP27
  • hsp27
  • hsp28
  • HSP28
  • HSPB1
  • id:ibd2821
  • LOH11CR1K
  • MKBP
  • sb:cb660
  • SRP27
  • zgc:103437
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Hamster, Human, Mouse (Murine), Rat (Rattus)
754
269
260
85
77
74
71
52
40
37
24
23
18
8
4
4
4
2
2
1
1
1
Host
Mouse
481
311
3
Clonality
Monoclonal
Conjugate
This HSPB1 antibody is un-conjugated
32
31
23
17
13
13
11
10
10
9
7
7
7
6
6
6
6
6
6
5
5
5
5
5
5
5
5
5
5
3
3
3
3
3
3
3
3
2
2
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
669
290
268
235
228
188
179
128
66
34
18
15
5
2
2
2
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN361756
$ 334.40
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.11990236 ABIN263953 IHC (fro) IF IP WB Mouse IgG1 Log in to see 8A7 8
0.11990236 ABIN1027725 ICC IF WB Rabbit Log in to see Polyclonal 7
0.11990236 ABIN126812 IHC (fro) WB Mouse IgG2a pSer82 Log in to see 5B9
0.11990236 ABIN1531868 ELISA WB Rabbit IgG pSer82 Log in to see Polyclonal
0.11990236 ABIN1532899 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.11990236 ABIN2854951 ICC FACS IF IP IHC (p) WB Rabbit IgG Center Log in to see Polyclonal
0.11990236 ABIN263952 IHC (fro) IF IP WB Mouse IgG1 Log in to see 8A7
0.11990236 ABIN498746 IHC (p) WB Rabbit pSer82 Log in to see Polyclonal
0.11990236 ABIN2706342 IP IHC WB Rabbit Center Log in to see Polyclonal
0.11990236 ABIN498463 IHC (p) WB Rabbit Log in to see Polyclonal
0.11990236 ABIN1873103 IF WB Rabbit IgG Log in to see Polyclonal
0.11990236 ABIN1107612 EIA IHC (p) WB Rabbit IgG Log in to see Polyclonal
0.11990236 ABIN2440372 IF IHC WB Rabbit IgG pSer15 Log in to see Polyclonal
0.11990236 ABIN4319980 ICC IF IHC IHC (p) WB Rabbit AA 80-150, Internal Region Log in to see Polyclonal 2
0.11990236 ABIN3020760 WB Rabbit IgG Log in to see Polyclonal
0.11990236 ABIN3185106 ELISA IHC WB Rabbit IgG Ser505 Log in to see Polyclonal
0.11990236 ABIN3182035 ELISA IHC WB Rabbit IgG pSer82 Log in to see Polyclonal
0.11990236 ABIN410085 ICC IF IHC (p) IP IHC WB Mouse IgG1 Log in to see 8A7
0.11990236 ABIN1870244 IHC WB Rabbit IgG pSer15 Log in to see Polyclonal
0.11990236 ABIN1859221 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 1-209 Log in to see Polyclonal

Top referenced anti-HSPB1 antibody for Western Blotting

Similar anti-HSPB1 Antibodies

Application / Reactivity Human Rat (Rattus) Dog (Canine) Mouse (Murine) Cow (Bovine) Hamster Guinea Pig
Western Blotting (WB) 637 Antibodies 225 Antibodies 49 Antibodies 227 Antibodies 69 Antibodies 36 Antibodies 39 Antibodies
Radioimmunoassay (RIA) 2 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Immunoprecipitation (IP) 161 Antibodies 79 Antibodies 80 Antibodies 38 Antibodies 36 Antibodies 35 Antibodies 34 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 213 Antibodies 120 Antibodies 122 Antibodies

General

Antigen Heat Shock 27kDa Protein 1 (HSPB1) Antibodies
Reactivity Cow (Bovine), Dog (Canine), Guinea Pig, Hamster, Human, Mouse (Murine), Rat (Rattus)
(754), (269), (260), (85), (77), (74), (71), (52), (40), (37), (24), (23), (18), (8), (4), (4), (4), (2), (2), (1), (1), (1)
Host Mouse
(481), (311), (3)
Clonality (Clone)
Monoclonal   ( )
Conjugate This HSPB1 antibody is un-conjugated
(32), (31), (23), (17), (13), (13), (11), (10), (10), (9), (7), (7), (7), (6), (6), (6), (6), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(669), (290), (268), (235), (228), (188), (179), (128), (66), (34), (18), (15), (5), (2), (2), (2)
Pubmed 9 references available
Supplier Log in to see

Product Details anti-HSPB1 Antibody

Target Details HSPB1 Application Details Handling References for anti-HSPB1 Antibody (ABIN361756) Images
Specificity Detects ~25 kDa or ~27 kDa. Recognizes HSP25 and HSP27, cross reacts with alpha B crystallin.
Sensitivity A 1:5000 dilution of SMC-114 was sufficient for detection of hsp27 in 20 µg of HeLa cell lysate by ECL immunoblot analysis.
Purification Protein G Purified
Immunogen HSP27 peptide
Clone 8A7

Target Details HSPB1

Product Details anti-HSPB1 Antibody Application Details Handling References for anti-HSPB1 Antibody (ABIN361756) Images back to top
Antigen
Alternative Name HSP27 (HSPB1 Antibody Abstract)
Background HSP25 is the mouse homologue of the human HSP27 protein, a member of the small heat shock protein family comprised of a diverse group of proteins from ~15 to >30 kDa(1). The basic structure of most sHSPs is a homologous and highly conserved amino acid sequence, with an α-crystallin-domain at the C-terminus and the WD/EPF domain at the less conserved N-terminus. This N-terminus is essential for the development of high molecular oligomers (2, 3). HSP27-oligomers consist of stable dimers formed by as many as 8-40 HSP27 protein monomers (4). The oligomerization status is connected with the chaperone activity: aggregates of large oligomers have high chaperone activity, whereas dimers have no chaperone activity (5). HSP27 is localized to the cytoplasm of unstressed cells but can redistribute to the nucleus in response to stress, where it may function to stabilize DNA and/or the nuclear membrane. It can be rapidly phosphorylated in response to physiological stimuli relevant to the cell type examined. Thus, HSP27 has been suggested to be an important intermediate in second messenger-mediated signaling pathways (6). Other functions include chaperone activity (as mentioned above), thermo-tolerance in vivo, inhibition of apoptosis, and signal transduction. Specifically, in vitro, it acts as an ATP-independent chaperone by inhibiting protein aggregation and by stabilizing partially denatured proteins, which ensures refolding of the HSP70 complex. HSP27 is also involved in the apoptotic signaling pathway because it interferes with the activation of cytochrome c/Apaf-1/dATP complex, thereby inhibiting the activation of procaspase-9. It is also hypothesized that HSP27 may serve some role in cross-bridge formation between actin and myosin (7). And finally, HSP27 is also thought to be involved in the process of cell differentiation. The up-regulation of HSP27 correlates with the rate of phosphorylation and with an increase of large oligomers. It is possible that HSP27 may play a crucial role in termination of growth (8).
Cellular Localization: Cytoplasm | Nucleus | Cytoskeleton | Spindle
Gene ID 3315
NCBI Accession NP_001532
UniProt P04792
Research Area Heat Shock Proteins
Pathways MAPK Signaling

Application Details

Product Details anti-HSPB1 Antibody Target Details HSPB1 Handling References for anti-HSPB1 Antibody (ABIN361756) Images back to top
Application Notes Recommended Dilution: WB (1:5000), ICC/IF (1:200), IHC (1:100), optimal dilutions for assays should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details References for anti-HSPB1 Antibody (ABIN361756) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer PBS, 50 % glycerol, 0.09 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C

References for anti-HSPB1 Antibody (ABIN361756)

Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details Handling Images back to top
Background publications

Arrigo: "In search of the molecular mechanism by which small stress proteins counteract apoptosis during cellular differentiation." in: Journal of cellular biochemistry, Vol. 94, Issue 2, pp. 241-6, 2005

Van Montfort, Slingsby, Vierling: "Structure and function of the small heat shock protein/alpha-crystallin family of molecular chaperones." in: Advances in protein chemistry, Vol. 59, pp. 105-56, 2002

Jia, Ransom, Shibanuma, Liu, Welsh, Smoyer: "Identification and characterization of hic-5/ARA55 as an hsp27 binding protein." in: The Journal of biological chemistry, Vol. 276, Issue 43, pp. 39911-8, 2001

Sarto, Binz, Mocarelli: "Heat shock proteins in human cancer." in: Electrophoresis, Vol. 21, Issue 6, pp. 1218-26, 2000

Kim, Kim, Kim: "Crystal structure of a small heat-shock protein." in: Nature, Vol. 394, Issue 6693, pp. 595-9, 1998

Ehrnsperger, Gräber, Gaestel, Buchner: "Binding of non-native protein to Hsp25 during heat shock creates a reservoir of folding intermediates for reactivation." in: The EMBO journal, Vol. 16, Issue 2, pp. 221-9, 1997

Welsh, Wu, Parvinen, Gilmont: "Variation in expression of hsp27 messenger ribonucleic acid during the cycle of the seminiferous epithelium and co-localization of hsp27 and microfilaments in Sertoli cells of the rat." in: Biology of reproduction, Vol. 55, Issue 1, pp. 141-51, 1997

Ciocca, Oesterreich, Chamness, McGuire, Fuqua: "Biological and clinical implications of heat shock protein 27,000 (Hsp27): a review." in: Journal of the National Cancer Institute, Vol. 85, Issue 19, pp. 1558-70, 1993

Welch: "Phorbol ester, calcium ionophore, or serum added to quiescent rat embryo fibroblast cells all result in the elevated phosphorylation of two 28,000-dalton mammalian stress proteins." in: The Journal of biological chemistry, Vol. 260, Issue 5, pp. 3058-62, 1985

Images

Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details Handling References for anti-HSPB1 Antibody (ABIN361756) back to top
Supplier Images
 image for anti-HSPB1 antibody (Heat Shock 27kDa Protein 1) (ABIN361756) Hsp25 Hsp27 (8A7), somites of a rat embryo Courtesy of Mike Welsh, UMich .
Western Blotting (WB) image for anti-HSPB1 antibody (Heat Shock 27kDa Protein 1) (ABIN361756) SMC 114 Rat LungTissue 10ug Hsp25 WB 1 in 1000.