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|Antigen||Heat Shock 27kDa Protein 1 (HSPB1) Antibodies|
|Epitope||AA 77-87, pSer82 Alternatives|
|Reactivity||Cow (Bovine), Dog (Canine), Horse (Equine), Human, Monkey, Pig (Porcine), Rabbit Alternatives|
|Conjugate||This HSPB1 antibody is un-conjugated Alternatives|
Immunoprecipitation (IP), Western Blotting (WB)
|Supplier||Log in to see|
Product Details anti-HSPB1 AntibodyTarget Details HSPB1 Application Details Handling Images
|Specificity||Detects an ~27kD protein, corresponding to the apparent molecular mass of phosphorylated Hsp27 (Ser82) on SDS-PAGE immunoblots. Species cross-reactivity: human, bovine, canine, chicken, guinea pig and sheep. This antibody is specific for phosphorylated Hsp27 (Ser82) and does not exhibit reactivity with non-phosphorylated Hsp27. Specificity for phospho-Hsp27 was demonstrated in peptide competition studies with phosphorylated and non-phosphorylated peptides.|
An 11 residue synthetic peptide, LSRQL[pS]SGVSE(C) corresponding to amino acids 77-87 of human Hsp27 with a cysteine (C) residue added and the peptide coupled to KLH.
Type of Immunogen: Synthetic peptide
Target Details HSPB1Product Details anti-HSPB1 Antibody Application Details Handling Images back to top
|Alternative Name||HSPB1 / HSP27 (HSPB1 Antibody Abstract)|
Name/Gene ID: HSPB1
Synonyms: HSPB1, 28 kDa heat shock protein, Heat shock 27 kDa protein, Heat shock 27kD protein 1, Heat shock 27 kDa protein 1, HSP27, HSP28, HSP 27, Hsp25, SRP27, Stress-responsive protein 27, CMT2F, Heat shock 27 kd protein, Heat shock protein beta-1, HMN2B, HS.76067
|Pathways||MAPK Signaling, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling|
Application DetailsProduct Details anti-HSPB1 Antibody Target Details HSPB1 Handling Images back to top
Approved: IP (1:50), WB
Usage: Suitable for use in Western Blot and Immunoprecipitation. Western Blot (Colorimetric): 1:500. Immunoprecipitation: 1:50.
|Restrictions||For Research Use only|
HandlingProduct Details anti-HSPB1 Antibody Target Details HSPB1 Application Details Images back to top
|Buffer||40 % glycerol.|
|Handling Advice||Avoid repeat freeze-thaw cycles.|
|Storage||4 °C,-20 °C|
Long term: -20°C
Short term: +4°C. Avoid repeat freeze-thaw cycles.