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anti-Rat (Rattus) HSPB1 antibody for ELISA

Recommended HSPB1 Antibody (supplied by: Log in to see )

Antigen
Heat Shock 27kDa Protein 1 (HSPB1) Antibodies
  • 27kDa
  • 2810021G24Rik
  • cb153
  • cb660
  • cmt2f
  • CMT2F
  • HMN2B
  • hs.76067
  • HS.76067
  • Hs.78846
  • hsp1
  • Hsp25
  • hsp25
  • Hsp27
  • HSP27
  • hsp27
  • hsp28
  • HSP28
  • HSPB1
  • id:ibd2821
  • LOH11CR1K
  • MKBP
  • sb:cb660
  • SRP27
  • zgc:103437
Reactivity
Human, Rat (Rattus)
753
273
262
87
79
76
73
54
42
39
24
22
18
8
4
4
4
2
2
1
1
1
Host
Mouse
480
312
3
Clonality
Monoclonal
Conjugate
This HSPB1 antibody is un-conjugated
32
31
23
17
13
13
11
10
10
9
7
7
7
6
6
6
6
6
6
6
5
5
5
5
5
5
5
5
5
5
3
3
3
3
3
3
3
2
2
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
669
290
265
238
231
191
183
128
68
34
18
15
5
2
2
2
Supplier
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Catalog No. ABIN969199
$ 430.10
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.3308015 ABIN1845754 ICC FACS IF IHC ELISA WB Mouse IgG1 Log in to see
0.3308015 ABIN4319976 ELISA FACS ICC IF IHC WB Mouse IgG1 Log in to see 5D7
0.0012043115 ABIN1531868 ELISA WB Rabbit IgG pSer82 Log in to see Polyclonal
0.0012043115 ABIN1532899 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.0012043115 ABIN966308 FACS IF IHC ELISA WB Mouse IgG1 Log in to see 5D7 2
0.0012043115 ABIN3182035 ELISA IHC WB Rabbit IgG pSer82 Log in to see Polyclonal
0.0012043115 ABIN3185106 ELISA IHC WB Rabbit IgG Ser505 Log in to see Polyclonal
0.0012043115 ABIN1735607 IHC (p) ELISA WB Rabbit IgG Internal Region Log in to see Polyclonal
0.0012043115 ABIN1585689 IHC ELISA WB Rabbit Middle Region Log in to see Polyclonal
0.0012043115 ABIN4880328 ELISA FACS ICC IHC WB Mouse IgG1 Log in to see 5D7
0.0012043115 ABIN865415 FACS IF IHC ELISA WB Mouse IgG1 Log in to see 5D7
0.0012043115 ABIN4320043 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Alexa Fluor 700 Mouse IgG1 kappa Log in to see SPM252
0.0012043115 ABIN4320040 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Alexa Fluor 405 Mouse IgG1 kappa Log in to see SPM252
0.0012043115 ABIN4320044 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Allophycocyanin Mouse IgG1 kappa Log in to see SPM252
0.0012043115 ABIN4320042 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Alexa Fluor 647 Mouse IgG1 kappa Log in to see SPM252
0.0012043115 ABIN4320041 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Alexa Fluor 488 Mouse IgG1 kappa Log in to see SPM252
0.0012043115 ABIN4320045 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Biotin Mouse IgG1 kappa Log in to see SPM252
0.0012043115 ABIN4320047 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 680 Mouse IgG1 kappa Log in to see SPM252
0.0012043115 ABIN4320048 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 488 Mouse IgG1 kappa Log in to see SPM252
0.0012043115 ABIN4320046 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 650 Mouse IgG1 kappa Log in to see SPM252

Top referenced anti-HSPB1 antibody for ELISA

  • Human Monoclonal HSPB1 Primary Antibody for FACS, IF - ABIN966308 (2 Publications): Langer, Ott, Specht, Becker, Lordick, Burian, Herrmann, Schrattenholz, Cahill, Schwaiger, Hofler, Wester: Protein expression profiling in esophageal adenocarcinoma patients indicates association of heat-shock protein 27 expression and chemotherapy response. in Clinical cancer research : an official journal of the American Association for Cancer Research 2008 (PubMed)

Similar anti-HSPB1 Antibodies

Application / Reactivity Rat (Rattus) Human
ELISA 43 Antibodies 255 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies 15 Antibodies
Flow Cytometry (FACS) 102 Antibodies 124 Antibodies
Immunocytochemistry (ICC) 125 Antibodies
Immunoelectron Microscopy (IEM) 2 Antibodies
Immunofluorescence (IF) 146 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies
Immunohistochemistry (IHC) 91 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 49 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 125 Antibodies
Immunoprecipitation (IP) 82 Antibodies
Western Blotting (WB) 227 Antibodies
Dot Blot (DB) 18 Antibodies
Immunochromatography (IC) 4 Antibodies

General

Antigen Heat Shock 27kDa Protein 1 (HSPB1) Antibodies
Reactivity Human, Rat (Rattus)
(753), (273), (262), (87), (79), (76), (73), (54), (42), (39), (24), (22), (18), (8), (4), (4), (4), (2), (2), (1), (1), (1)
Host Mouse
(480), (312), (3)
Clonality (Clone)
Monoclonal   ( )
Conjugate This HSPB1 antibody is un-conjugated
(32), (31), (23), (17), (13), (13), (11), (10), (10), (9), (7), (7), (7), (6), (6), (6), (6), (6), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (3), (3), (3), (3), (3), (3), (3), (2), (2)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(669), (290), (265), (238), (231), (191), (183), (128), (68), (34), (18), (15), (5), (2), (2), (2)
Pubmed 1 reference available
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Product Details anti-HSPB1 Antibody

Target Details HSPB1 Application Details Handling References for anti-HSPB1 Antibody (ABIN969199) Images
Purification Ascites
Immunogen Purified recombinant fragment of human HSP27 expressed in E. coli.
Clone 5D7
Isotype IgG1

Target Details HSPB1

Product Details anti-HSPB1 Antibody Application Details Handling References for anti-HSPB1 Antibody (ABIN969199) Images back to top
Antigen
Alternative Name HSP27 (HSPB1 Antibody Abstract)
Background The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN). (provided by RefSeq) Tissue specificity: Detected in all tissues tested: skeletal muscle, heart, aorta, large intestine, small intestine, stomach, esophagus, bladder, adrenal gland, thyroid, pancreas, testis, adipose tissue, kidney, liver, spleen, cerebral cortex, blood serum and cerebrospinal fluid. Highest levels are found in the heart and in tissues composed of striated and smooth muscle.
Molecular Weight 27 kDa
Gene ID 3315
HGNC 3315
Research Area Heat Shock Proteins
Pathways MAPK Signaling

Application Details

Product Details anti-HSPB1 Antibody Target Details HSPB1 Handling References for anti-HSPB1 Antibody (ABIN969199) Images back to top
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000, IHC: 1/200 - 1/1000, ICC: 1/200 - 1/1000, FCM: 1/200 - 1/400
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only

Handling

Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details References for anti-HSPB1 Antibody (ABIN969199) Images back to top
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.

References for anti-HSPB1 Antibody (ABIN969199)

Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details Handling Images back to top
Product cited in:

Langer, Ott, Specht, Becker, Lordick, Burian, Herrmann, Schrattenholz, Cahill, Schwaiger, Hofler, Wester: "Protein expression profiling in esophageal adenocarcinoma patients indicates association of heat-shock protein 27 expression and chemotherapy response." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 14, Issue 24, pp. 8279-87, 2008

Images

Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details Handling References for anti-HSPB1 Antibody (ABIN969199) back to top
Supplier Images
ELISA image for anti-HSPB1 antibody (Heat Shock 27kDa Protein 1) (ABIN969199) Figure 1: Red: Control Antigen (100 ng), Purple: Antigen (10ng), Green: Antigen (50 n...
Flow Cytometry (FACS) image for anti-HSPB1 antibody (Heat Shock 27kDa Protein 1) (ABIN969199) Figure 2: Flow cytometric analysis of HepG2 cells using HSP27 mouse mAb (green) and n...
Immunocytochemistry (ICC) image for anti-HSPB1 antibody (Heat Shock 27kDa Protein 1) (ABIN969199) Figure 3: Immunofluorescence analysis of Hela cells using HSP27 mouse mAb (green) . B...
Immunohistochemistry (IHC) image for anti-HSPB1 antibody (Heat Shock 27kDa Protein 1) (ABIN969199) Figure 4: Immunohistochemical analysis of paraffin-embedded brain tissues (left) and ...
Immunohistochemistry (IHC) image for anti-HSPB1 antibody (Heat Shock 27kDa Protein 1) (ABIN969199) Figure 5: Immunohistochemical analysis of paraffin-embedded breast cancer tissues (le...
Western Blotting (WB) image for anti-HSPB1 antibody (Heat Shock 27kDa Protein 1) (ABIN969199) Figure 6: Western blot analysis using HSP27 mouse mAb against Hela (1), A549 (2), Jur...