anti-Rat (Rattus) HSPB1 antibody for Immunofluorescence

Recommended HSPB1 Antibody (supplied by: Log in to see )

Antigen
Heat Shock 27kDa Protein 1 (HSPB1) Antibodies
  • cb153
  • cb660
  • hsp1
  • hsp25
  • hsp27
  • id:ibd2821
  • sb:cb660
  • zgc:103437
  • HSPB1
  • cmt2f
  • hs.76067
  • hsp28
  • CMT2F
  • HMN2B
  • HS.76067
  • HSP27
  • HSP28
  • Hsp25
  • SRP27
  • Hs.78846
  • LOH11CR1K
  • MKBP
  • Hsp27
  • 27kDa
  • 2810021G24Rik
  • heat shock protein, alpha-crystallin-related, 1
  • heat shock 27kDa protein 1
  • heat shock 27kDa protein 2
  • heat shock protein 1
  • heat shock protein 2
  • heat shock protein 25
  • hspb1
  • HSPB1
  • HSP27
  • HSPB2
  • Hspb1
  • Hspb2
  • HSP25
Epitope
AA 80-150, Internal Region
86
74
56
45
21
15
12
12
10
10
10
10
6
6
5
5
5
5
5
4
4
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
830
279
275
96
85
71
68
66
36
30
29
26
18
11
11
9
6
3
2
2
2
2
1
1
1
Host
Rabbit
510
338
2
Clonality
Polyclonal
Conjugate
This HSPB1 antibody is un-conjugated
34
32
22
21
13
13
13
13
11
11
10
10
10
10
10
9
9
9
6
5
5
5
5
5
5
5
5
5
4
4
3
3
3
3
3
3
3
3
3
3
3
2
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
719
334
272
264
231
211
169
156
59
40
17
14
11
8
6
4
2
2
1
1
1
Supplier
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Catalog No. ABIN4319980
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
11.917223 ABIN2854951 ICC FACS IF IP IHC (p) WB Rabbit IgG Center Log in to see Polyclonal
11.917223 ABIN1873103 IF WB Rabbit IgG Log in to see Polyclonal
11.917223 ABIN4320008 CyTOF FACS ICC IF WB Mouse IgG1 Log in to see 6H11
11.917223 ABIN267333 ICC IF IHC WB Rabbit IgG Log in to see Polyclonal
11.917223 ABIN1107614 EIA IF IHC (p) WB Mouse IgG1 Log in to see 5D7 2
11.917223 ABIN4319976 ELISA FACS ICC IF IHC WB Mouse IgG1 Log in to see 5D7
11.917223 ABIN4320010 ICC IF WB Chicken Log in to see Polyclonal
11.917223 ABIN1870246 IF IHC WB Rabbit IgG pSer82 Log in to see Polyclonal
11.917223 ABIN2406882 IF IHC WB Rabbit IgG pSer82 Log in to see Polyclonal
1 ABIN361756 FACS ICC IF IHC IP WB Mouse IgG1 kappa Log in to see 8A7 9
1 ABIN1498733 ELISA FACS ICC IF IHC (p) WB Mouse IgG1 Log in to see 5D7
1 ABIN966308 FACS IF IHC ELISA WB Mouse IgG1 Log in to see 5D7 2
1 ABIN4320020 FACS ICC IF IHC (p) WB Mouse IgG1 kappa Log in to see SPM252
1 ABIN2481416 FACS ICC IF IHC IP WB Atto 655 Mouse IgG1 kappa Log in to see 8A7
1 ABIN2481412 FACS ICC IF IHC IP WB Atto 488 Mouse IgG1 kappa Log in to see 8A7
1 ABIN2481418 FACS ICC IF IHC IP WB Atto 700 Mouse IgG1 kappa Log in to see 8A7
1 ABIN2481415 FACS ICC IF IHC IP WB Atto 633 Mouse IgG1 kappa Log in to see 8A7
1 ABIN2481421 FACS ICC IF IHC IP WB Biotin Mouse IgG1 kappa Log in to see 8A7
1 ABIN2481420 FACS ICC IF IHC IP WB APC Mouse IgG1 kappa Log in to see 8A7
1 ABIN2481422 FACS ICC IF IHC IP WB FITC Mouse IgG1 kappa Log in to see 8A7

General

Antigen Heat Shock 27kDa Protein 1 (HSPB1) Antibodies
Epitope AA 80-150, Internal Region
(86), (74), (56), (45), (21), (15), (12), (12), (10), (10), (10), (10), (6), (6), (5), (5), (5), (5), (5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(830), (279), (275), (96), (85), (71), (68), (66), (36), (30), (29), (26), (18), (11), (11), (9), (6), (3), (2), (2), (2), (2), (1), (1), (1)
Host Rabbit
(510), (338), (2)
Clonality
Polyclonal
Conjugate This HSPB1 antibody is un-conjugated
(34), (32), (22), (21), (13), (13), (13), (13), (11), (11), (10), (10), (10), (10), (10), (9), (9), (9), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(719), (334), (272), (264), (231), (211), (169), (156), (59), (40), (17), (14), (11), (8), (6), (4), (2), (2), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-HSPB1 Antibody

Target Details HSPB1 Application Details Handling References for anti-HSPB1 antibody (ABIN4319980) Images
Purification Immunogen affinity purified
Immunogen A synthetic peptide made to an internal region of the human Hsp27 protein (within residues 80-150). [Swiss-Prot P04792]

Target Details HSPB1

Product Details anti-HSPB1 Antibody Application Details Handling References for anti-HSPB1 antibody (ABIN4319980) Images back to top
Antigen
Alternative Name HSP27 (HSPB1 Antibody Abstract)
Background Gene Symbol: HSPB1
Gene ID 3315
UniProt P04792, P14602
Research Area Heat Shock Proteins
Pathways MAPK Signaling, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling

Application Details

Product Details anti-HSPB1 Antibody Target Details HSPB1 Handling References for anti-HSPB1 antibody (ABIN4319980) Images back to top
Application Notes Western Blot 2 μg/mL, Immunohistochemistry 1:100, Immunocytochemistry/Immunofluorescence 1:100, Immunohistochemistry-Paraffin 1:100This HSP27 antibody is useful for ICC/IF, IHC-P, and Western blot where a band is seen ~27 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer ( pH 6.0) is recommended.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details References for anti-HSPB1 antibody (ABIN4319980) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer PBS and 30 % Glycerol
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

References for anti-HSPB1 antibody (ABIN4319980)

Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details Handling Images back to top
Product cited in:

López-González, Carmona, Arregui, Kovacs, Ferrer: "αB-crystallin and HSP27 in glial cells in tauopathies." in: Neuropathology : official journal of the Japanese Society of Neuropathology, Vol. 34, Issue 6, pp. 517-26, 2014 Further details: Immunohistochemistry (Paraffin-embedded Sections)

Stice, Chen, Kim, Jung, Tran, Liu, Knowlton: "17β-Estradiol, aging, inflammation, and the stress response in the female heart." in: Endocrinology, Vol. 152, Issue 4, pp. 1589-98, 2011 (Sample species: Rat (Rattus)). Further details: Western Blotting

Images

Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details Handling References for anti-HSPB1 antibody (ABIN4319980) back to top
Supplier Images
Western Blotting (WB) image for anti-Heat Shock 27kDa Protein 1 (HSPB1) (AA 80-150), (Internal Region) antibody (ABIN4319980) Western Blot: Hsp27 Antibody [NBP1-75477] - Analysis of HSP27 in human skeletal muscl...
Western Blotting (WB) image for anti-Heat Shock 27kDa Protein 1 (HSPB1) (AA 80-150), (Internal Region) antibody (ABIN4319980) Western Blot: Hsp27 Antibody [NBP1-75477] - analysis of Jurkat cell lysate (30ug) usi...
Immunofluorescence (IF) image for anti-Heat Shock 27kDa Protein 1 (HSPB1) (AA 80-150), (Internal Region) antibody (ABIN4319980) Immunocytochemistry/Immunofluorescence: Hsp27 Antibody [NBP1-75477] - HSP27 antibody ...
Immunohistochemistry (IHC) image for anti-Heat Shock 27kDa Protein 1 (HSPB1) (AA 80-150), (Internal Region) antibody (ABIN4319980) Immunohistochemistry: Hsp27 Antibody [NBP1-75477] - Staining of HSP27 in mouse heart ...