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anti-Rat (Rattus) HSPB1 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended HSPB1 Antibody (supplied by: Log in to see )

Antigen
Heat Shock 27kDa Protein 1 (HSPB1) Antibodies
  • 27kDa
  • 2810021G24Rik
  • cb153
  • cb660
  • cmt2f
  • CMT2F
  • HMN2B
  • hs.76067
  • HS.76067
  • Hs.78846
  • hsp1
  • Hsp25
  • hsp25
  • Hsp27
  • HSP27
  • hsp27
  • hsp28
  • HSP28
  • HSPB1
  • id:ibd2821
  • LOH11CR1K
  • MKBP
  • sb:cb660
  • SRP27
  • zgc:103437
Epitope
pSer82
67
67
53
40
25
22
16
16
14
13
12
8
6
5
5
5
5
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
761
273
264
85
77
75
71
53
41
38
24
23
18
8
4
4
4
2
2
1
1
1
Host
Rabbit
485
314
3
Clonality
Polyclonal
Conjugate
This HSPB1 antibody is un-conjugated
32
31
23
17
13
13
11
10
10
9
7
7
7
6
6
6
6
6
6
6
5
5
5
5
5
5
5
5
5
5
3
3
3
3
3
3
3
2
2
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
673
294
268
238
227
189
180
128
66
37
18
15
5
2
2
2
Supplier
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Catalog No. ABIN498746
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.23971483 ABIN498848 IHC (p) Rabbit pSer78, pSer82 Log in to see Polyclonal
0.23971483 ABIN498463 IHC (p) WB Rabbit Log in to see Polyclonal
0.23971483 ABIN1107613 IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal
0.23971483 ABIN1107612 EIA IHC (p) WB Rabbit IgG Log in to see Polyclonal
0.23971483 ABIN410085 ICC IF IHC (p) IP IHC WB Mouse IgG1 Log in to see 8A7
0.23971483 ABIN498937 IHC (p) Rabbit Log in to see Polyclonal
0.23971483 ABIN267333 ICC IF IHC (p) IHC WB Rabbit IgG Log in to see Polyclonal
0.23971483 ABIN4319975 IHC IHC (p) WB Rabbit pSer82 Log in to see Polyclonal
0.0008985382 ABIN197344 IHC (p) WB Rabbit Ser15 Log in to see Polyclonal 4
0.0008985382 ABIN672441 IF (p) IHC (p) WB Rabbit IgG AA 100-150 Log in to see Polyclonal 2
0.0008985382 ABIN2854951 ICC FACS IF IP IHC (p) WB Rabbit IgG Center Log in to see Polyclonal
0.0008985382 ABIN1107614 EIA IF IHC (p) WB Mouse IgG1 Log in to see 5D7 2
0.0008985382 ABIN3044184 ICC IHC (fro) IHC (p) WB Rabbit IgG AA 88-105, Middle Region Log in to see Polyclonal
0.0008985382 ABIN4319980 ICC IF IHC IHC (p) WB Rabbit AA 80-150, Internal Region Log in to see Polyclonal 2
0.0008985382 ABIN3043648 ICC IHC (fro) IHC (p) WB Mouse IgG1 Log in to see IAP-9
0.0008985382 ABIN672450 IHC (p) WB HRP Rabbit IgG AA 100-150 Log in to see Polyclonal
0.0008985382 ABIN1735607 IHC (p) ELISA WB Rabbit IgG Internal Region Log in to see Polyclonal
0.0008985382 ABIN685327 IHC (p) WB HRP Rabbit IgG pSer82 Log in to see Polyclonal
0.0008985382 ABIN672443 IHC (p) WB Biotin Rabbit IgG AA 100-150 Log in to see Polyclonal
0.0008985382 ABIN4320068 FACS IF IHC (p) WB Mouse IgG1 kappa Log in to see HSPB1-774

Top referenced anti-HSPB1 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Similar anti-HSPB1 Antibodies

Application / Reactivity Human Rat (Rattus) Mouse (Murine)
Western Blotting (WB) 641 Antibodies 227 Antibodies 229 Antibodies
Radioimmunoassay (RIA) 2 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Immunoprecipitation (IP) 161 Antibodies 79 Antibodies 80 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 213 Antibodies 120 Antibodies 122 Antibodies
Immunohistochemistry (IHC) 281 Antibodies 82 Antibodies 93 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 63 Antibodies 47 Antibodies 47 Antibodies

General

Antigen Heat Shock 27kDa Protein 1 (HSPB1) Antibodies
Epitope pSer82
(67), (67), (53), (40), (25), (22), (16), (16), (14), (13), (12), (8), (6), (5), (5), (5), (5), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(761), (273), (264), (85), (77), (75), (71), (53), (41), (38), (24), (23), (18), (8), (4), (4), (4), (2), (2), (1), (1), (1)
Host Rabbit
(485), (314), (3)
Clonality
Polyclonal
Conjugate This HSPB1 antibody is un-conjugated
(32), (31), (23), (17), (13), (13), (11), (10), (10), (9), (7), (7), (7), (6), (6), (6), (6), (6), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (3), (3), (3), (3), (3), (3), (3), (2), (2)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(673), (294), (268), (238), (227), (189), (180), (128), (66), (37), (18), (15), (5), (2), (2), (2)
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Product Details anti-HSPB1 Antibody

Target Details HSPB1 Application Details Handling Images
Specificity This antibody detects endogenous levels of p-HSP27 protein only when phosphorylated at Ser82.
Cross-Reactivity (Details) Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
Purification Affinity chromatography
Purity > 95 % by SDS-PAGE

Target Details HSPB1

Product Details anti-HSPB1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name HSPB1 / HSP27 (HSPB1 Antibody Abstract)
Background HSP 27 is a constitutively expressed cytoplasmic protein that co-localizes to the nucleus upon stress-induced insult. The intracellular concentration of HSP 27 increases several-fold after heat shock and other metabolic stresses, and is closely associated with the acquisition of thermotolerance. In addition to heat shock, cytokines and hormones are among the factors that stimulate the synthesis of HSP 27. MAP kinaseactivated protein kinase-2 phosphorylates HSP 27 on Serine residues Ser 15, Ser 78 and Ser 82, which are phosphorylated in vivo in response to growth factors and heat shock. Ser 15, Ser 78 and Ser 82 occur in the sequence motif RXXS, which is recognized by ribosomal protein S6 kinase II.Synonyms: 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, HSP25, HSP28, Heat shock 27 kDa protein, Heat shock protein beta-1, SRP27, Stress-responsive protein 27
Molecular Weight approx. 27 kDa
Gene ID 3315
NCBI Accession NP_001531
UniProt P04792
Pathways MAPK Signaling

Application Details

Product Details anti-HSPB1 Antibody Target Details HSPB1 Handling Images back to top
Application Notes Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details Images back to top
Concentration 1,0 mg/mL
Buffer Phosphate buffered saline (PBS), pH 7.2., 15 mM sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Images

Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details Handling back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-HSPB1 antibody (Heat Shock 27kDa Protein 1) (pSer82) (ABIN498746) Immunohistochemistry (IHC) analyzes of p-HSP27 antibody in paraffin-embedded h...
Western Blotting (WB) image for anti-HSPB1 antibody (Heat Shock 27kDa Protein 1) (pSer82) (ABIN498746) Western blot (WB) analyzes of p-HSP27 antibody in extracts from HUVEC TNF cells.