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anti-Rat (Rattus) HSPB1 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended HSPB1 Antibody (supplied by: Log in to see )

Antigen
Heat Shock 27kDa Protein 1 (HSPB1) Antibodies
  • cb153
  • cb660
  • hsp1
  • hsp25
  • hsp27
  • id:ibd2821
  • sb:cb660
  • zgc:103437
  • HSPB1
  • cmt2f
  • hs.76067
  • hsp28
  • CMT2F
  • HMN2B
  • HS.76067
  • HSP27
  • HSP28
  • Hsp25
  • SRP27
  • Hs.78846
  • LOH11CR1K
  • MKBP
  • Hsp27
  • 27kDa
  • 2810021G24Rik
  • heat shock protein, alpha-crystallin-related, 1
  • heat shock 27kDa protein 1
  • heat shock 27kDa protein 2
  • heat shock protein 1
  • heat shock protein 2
  • heat shock protein 25
  • hspb1
  • HSPB1
  • HSP27
  • HSPB2
  • Hspb1
  • Hspb2
  • HSP25
Epitope
AA 100-150
83
68
53
44
24
17
16
16
14
13
12
9
6
5
5
5
5
5
4
4
4
4
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
838
289
284
88
80
79
71
57
53
38
32
30
19
13
11
9
6
4
4
2
2
2
2
2
1
1
Host
Rabbit
510
350
3
Clonality
Polyclonal
Conjugate
This HSPB1 antibody is un-conjugated
34
34
23
20
13
13
13
13
12
11
10
10
10
9
9
9
9
9
6
5
5
5
5
5
5
5
5
5
4
4
3
3
3
3
3
3
3
3
3
3
3
2
Application
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
725
346
284
250
229
214
174
124
61
39
18
15
9
8
6
4
2
2
2
Options
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Catalog No. ABIN672441
$ 307.45
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
12.931439 ABIN498848 IHC (p) Rabbit pSer78, pSer82 Log in to see Polyclonal
12.931439 ABIN498746 IHC (p) WB Rabbit pSer82 Log in to see Polyclonal
12.931439 ABIN1107612 EIA IHC (p) WB Rabbit IgG Log in to see Polyclonal
12.931439 ABIN1107613 IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal
12.931439 ABIN498463 IHC (p) WB Rabbit Log in to see Polyclonal
12.931439 ABIN498937 IHC (p) Rabbit Log in to see Polyclonal
12.931439 ABIN4319975 IHC IHC (p) WB Rabbit pSer82 Log in to see Polyclonal
1 ABIN197344 IHC (p) WB Rabbit Ser15 Log in to see Polyclonal 4
1 ABIN4319980 ICC IF IHC IHC (p) WB Rabbit AA 80-150, Internal Region Log in to see Polyclonal 2
1 ABIN2854951 ICC FACS IF IP IHC (p) WB Rabbit IgG Center Log in to see Polyclonal
1 ABIN1107614 EIA IF IHC (p) WB Mouse IgG1 Log in to see 5D7 2
1 ABIN3044184 ICC IHC (fro) IHC (p) WB Rabbit IgG AA 88-105, Middle Region Log in to see Polyclonal
1 ABIN2878840 IHC (p) ELISA WB Rabbit IgG AA 48-97 Log in to see Polyclonal
1 ABIN1498733 ELISA FACS ICC IF IHC (p) WB Mouse IgG1 Log in to see 5D7
1 ABIN1735607 IHC (p) ELISA WB Rabbit IgG Internal Region Log in to see Polyclonal
1 ABIN3043648 ICC IHC (fro) IHC (p) WB Mouse IgG1 Log in to see IAP-9
1 ABIN672450 IHC (p) WB HRP Rabbit IgG AA 100-150 Log in to see Polyclonal
1 ABIN1493063 IHC (p) Rabbit C-Term Log in to see Polyclonal
1 ABIN3025020 FACS IF IHC (p) WB Mouse IgG1, kappa Log in to see HSPB1-774
1 ABIN4320021 FACS ICC IF IHC (p) WB Mouse IgG1 kappa Log in to see SPM252

Top referenced anti-HSPB1 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Similar anti-HSPB1 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
Cytometry by Time of Flight (CyTOF) 8 Antibodies 8 Antibodies
ELISA 60 Antibodies 56 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies 2 Antibodies
Flow Cytometry (FACS) 103 Antibodies 95 Antibodies
Immunocytochemistry (ICC) 105 Antibodies
Immunoelectron Microscopy (IEM) 2 Antibodies
Immunofluorescence (IF) 132 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 4 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 26 Antibodies
Immunohistochemistry (IHC) 88 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 45 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 115 Antibodies
Immunoprecipitation (IP) 64 Antibodies
Western Blotting (WB) 229 Antibodies
Immunochromatography (IC) 1 Antibodies

General

Antigen Heat Shock 27kDa Protein 1 (HSPB1) Antibodies
Epitope AA 100-150
(83), (68), (53), (44), (24), (17), (16), (16), (14), (13), (12), (9), (6), (5), (5), (5), (5), (5), (4), (4), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(838), (289), (284), (88), (80), (79), (71), (57), (53), (38), (32), (30), (19), (13), (11), (9), (6), (4), (4), (2), (2), (2), (2), (2), (1), (1)
Host Rabbit
(510), (350), (3)
Clonality
Polyclonal
Conjugate This HSPB1 antibody is un-conjugated
(34), (34), (23), (20), (13), (13), (13), (13), (12), (11), (10), (10), (10), (9), (9), (9), (9), (9), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2)
Application Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(725), (346), (284), (250), (229), (214), (174), (124), (61), (39), (18), (15), (9), (8), (6), (4), (2), (2), (2)
Pubmed 2 references available
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Product Details anti-HSPB1 Antibody

Target Details HSPB1 Application Details Handling References for anti-HSPB1 Antibody (ABIN672441) Images
Purification Purified by Protein A.
Immunogen KLH conjugated synthetic peptide derived from human HSP27
Isotype IgG

Target Details HSPB1

Product Details anti-HSPB1 Antibody Application Details Handling References for anti-HSPB1 Antibody (ABIN672441) Images back to top
Antigen
Alternative Name HSP27 (HSPB1 Antibody Abstract)
Background

Heat Shock Protein 27 (HSP27) is a 27 kDa member of a family of proteins whose expression and function are stimulated by heat shock and other stress stimuli. A major function of these proteins is to serve as chaperones that bind to and stabilize the active conformation of other proteins. HSP27, along with other members of the small HSP group, possesses a C-terminal Alpha-crystalline homology domain. HSP27 is localized to the cytoplasm of unstressed cells but can redistribute to the nucleus in response to stress, where it may function to stabilize DNA and/or the nuclear membrane. Cytoplasmic HSP27 exists in multiple complexes. One complex consists of HSP27, Akt (PKB), MAPKAP-kinase 2, and p38 MAPK. The presence of HSP27 in this complex is required for Akt activation by stress stimuli. Another complex consists of HSP27 and the IKK complex. HSP27 is also an actin capping protein that binds to the barbed (growing) ends of actin filaments, thereby inhibiting filament extension. Phosphorylation of HSP27 on serine 82 by MAPKAP-kinase 2 leads to HSP27 dissociation from the Akt/MAPKAP-kinase 2/p38 complex and from actin filaments, and stimulates HSP27 binding to the IKK complex.

Subcellular location: Cytoplasm, Nucleus


Synonyms: CMT2F, HMN2B, HSP27, HSP28, Hsp25, SRP27, HS.7667, HEL-S-12, Heat shock protein beta-1, HspB1, 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, Heat shock 27 kDa protein, HSP 27, Stress-responsive protein 27
Gene ID 3315
UniProt P04792
Research Area Heat Shock Proteins
Pathways MAPK Signaling, Regulation of Actin Filament Polymerization

Application Details

Product Details anti-HSPB1 Antibody Target Details HSPB1 Handling References for anti-HSPB1 Antibody (ABIN672441) Images back to top
Application Notes WB(1:100-1000), IHC-P(1:100-500), IF(IHC-P)(1:50-200)
Restrictions For Research Use only

Handling

Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details References for anti-HSPB1 Antibody (ABIN672441) Images back to top
Format Liquid
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20°C
Expiry Date 12 months

References for anti-HSPB1 Antibody (ABIN672441)

Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details Handling Images back to top
Product cited in:

Gao, Liu, Chen, Lv, Wu, Mi, Wang: "Comparative study of Hsp27, GSK3?, Wnt1 and PRDX3 in Hirschsprung's disease." in: International journal of experimental pathology, Vol. 95, Issue 3, pp. 229-37, 2014

Wang, Yang, Liu, Zhou, Wu, Qiao, Li, Wang: "Dietary supplementation with the probiotic Lactobacillus fermentum I5007 and the antibiotic aureomycin differentially affects the small intestinal proteomes of weanling piglets." in: The Journal of nutrition, Vol. 142, Issue 1, pp. 7-13, 2011

Images

Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details Handling References for anti-HSPB1 Antibody (ABIN672441) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-HSPB1 antibody (Heat Shock 27kDa Protein 1) (AA 100-150) (ABIN672441) Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-HSP27/HspB1/H...
Immunohistochemistry (IHC) image for anti-HSPB1 antibody (Heat Shock 27kDa Protein 1) (AA 100-150) (ABIN672441) Formalin-fixed and paraffin embedded rat brain labeled with (ABIN672441) Rabbit Ant...
SDS-PAGE (SDS) image for anti-HSPB1 antibody (Heat Shock 27kDa Protein 1) (AA 100-150) (ABIN672441) L1 mouse brain lysates L2 mouse liver lysates probed with Anti HSP27/HspB1/HSP25 Poly...
Immunofluorescence (IF) image for anti-HSPB1 antibody (Heat Shock 27kDa Protein 1) (AA 100-150) (ABIN672441) Formalin-fixed and paraffin-embedded rat brain labeled with Anti-HSP27/HspB1/HSP25 Po...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-HSPB1 antibody (Heat Shock 27kDa Protein 1) (AA 100-150) (ABIN672441) Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-HSP27/HspB1/H...