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anti-Sheep (Ovine) HSPB1 antibody for Western Blotting

Recommended HSPB1 Antibody (supplied by: Log in to see )

Antigen
Heat Shock 27kDa Protein 1 (HSPB1) Antibodies
  • cb153
  • cb660
  • hsp1
  • hsp25
  • hsp27
  • id:ibd2821
  • sb:cb660
  • zgc:103437
  • HSPB1
  • cmt2f
  • hs.76067
  • hsp28
  • CMT2F
  • HMN2B
  • HS.76067
  • HSP27
  • HSP28
  • Hsp25
  • SRP27
  • Hs.78846
  • LOH11CR1K
  • MKBP
  • Hsp27
  • 27kDa
  • 2810021G24Rik
  • heat shock protein, alpha-crystallin-related, 1
  • heat shock 27kDa protein 1
  • heat shock 27kDa protein 2
  • heat shock protein 1
  • heat shock protein 2
  • heat shock protein 25
  • hspb1
  • HSPB1
  • HSP27
  • HSPB2
  • Hspb1
  • Hspb2
  • HSP25
Reactivity
Chicken, Chimpanzee, Human, Mouse (Murine), Rat (Rattus), Sheep (Ovine)
796
272
263
84
76
75
71
53
41
38
24
23
18
7
4
4
4
2
2
1
1
1
Host
Mouse
505
330
3
Clonality
Monoclonal
Conjugate
This HSPB1 antibody is un-conjugated
34
33
23
19
13
13
12
12
11
11
9
9
9
8
8
8
8
8
6
5
5
5
5
5
5
5
5
5
5
3
3
3
3
3
3
3
3
2
2
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
694
327
280
232
223
213
162
123
62
34
18
15
8
6
4
2
2
2
Supplier
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Catalog No. ABIN3026467
$ 361.90
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN3025020 FACS IF IHC (p) WB Mouse IgG1, kappa Log in to see HSPB1-774
1 ABIN3025018 FACS IF IHC WB Mouse IgG1, kappa Log in to see SPM252
1 ABIN4320025 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG1 kappa Log in to see G3-1
1 ABIN4320021 FACS ICC IF IHC (p) WB Mouse IgG1 kappa Log in to see SPM252
1 ABIN4320022 FACS ICC IF IHC (p) WB Mouse IgG1 kappa Log in to see SPM252
1 ABIN4320020 FACS ICC IF IHC (p) WB Mouse IgG1 kappa Log in to see SPM252
1 ABIN4320023 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG1 kappa Log in to see G3-1
1 ABIN4320024 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG1 kappa Log in to see G3-1
1 ABIN4320066 FACS IF IHC (p) WB Mouse IgG1 kappa Log in to see HSPB1-774
1 ABIN4320068 FACS IF IHC (p) WB Mouse IgG1 kappa Log in to see HSPB1-774
1 ABIN4320067 FACS IF IHC (p) WB Mouse IgG1 kappa Log in to see HSPB1-774
1 ABIN4320032 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 680 Mouse IgG1 kappa Log in to see G3-1
1 ABIN4320057 ELISA FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 405 Mouse IgG1 kappa Log in to see G3-1
1 ABIN4320058 ELISA FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 488 Mouse IgG1 kappa Log in to see G3-1
1 ABIN4320059 ELISA FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 647 Mouse IgG1 kappa Log in to see G3-1
1 ABIN4320060 ELISA FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 700 Mouse IgG1 kappa Log in to see G3-1
1 ABIN4320061 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Biotin Mouse IgG1 kappa Log in to see G3-1
1 ABIN4320031 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 650 Mouse IgG1 kappa Log in to see G3-1
1 ABIN4320062 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 488 Mouse IgG1 kappa Log in to see G3-1
1 ABIN4320034 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 755 Mouse IgG1 kappa Log in to see G3-1

Similar anti-HSPB1 Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus) Chicken Sheep (Ovine) Chimpanzee
Cytometry by Time of Flight (CyTOF) 8 Antibodies 8 Antibodies 8 Antibodies 6 Antibodies 6 Antibodies
Simple Western (SimWes) 4 Antibodies
Western Blotting (WB) 660 Antibodies 217 Antibodies 219 Antibodies 73 Antibodies 65 Antibodies 6 Antibodies
Radioimmunoassay (RIA) 2 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Immunoprecipitation (IP) 152 Antibodies 61 Antibodies 37 Antibodies 62 Antibodies

General

Antigen Heat Shock 27kDa Protein 1 (HSPB1) Antibodies
Reactivity Chicken, Chimpanzee, Human, Mouse (Murine), Rat (Rattus), Sheep (Ovine)
(796), (272), (263), (84), (76), (75), (71), (53), (41), (38), (24), (23), (18), (7), (4), (4), (4), (2), (2), (1), (1), (1)
Host Mouse
(505), (330), (3)
Clonality (Clone)
Monoclonal   ( )
Conjugate This HSPB1 antibody is un-conjugated
(34), (33), (23), (19), (13), (13), (12), (12), (11), (11), (9), (9), (9), (8), (8), (8), (8), (8), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
(694), (327), (280), (232), (223), (213), (162), (123), (62), (34), (18), (15), (8), (6), (4), (2), (2), (2)
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Product Details anti-HSPB1 Antibody

Target Details HSPB1 Application Details Handling Images
Characteristics This antibody recognizes a 24-27 kDa estrogen-regulated protein, identified as heat shock protein 27 (hsp27). HSP27 was recently found to be identical to the estrogen-induced "p29" and "24K" protein. About 50 % of breast carcinomas are positive for HSP27, especially those that are also positive for estrogen and/or progesterone receptor. HSP27 has also been implicated in drug resistance in cancer cells.
Purification Protein G affinity chromatography
Immunogen Partially purified human HSP27 (earlier called 24K) from breast cancer MCF-7 cells was used as the immunogen for this antibody.
Clone G3-1
Isotype IgG1, kappa

Target Details HSPB1

Product Details anti-HSPB1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name HSP27 (HSPB1 Antibody Abstract)
Background This antibody recognizes a 24-27 kDa estrogen-regulated protein, identified as heat shock protein 27 (hsp27). HSP27 was recently found to be identical to the estrogen-induced ""p29"" and ""24K"" protein. About 50 % of breast carcinomas are positive for HSP27, especially those that are also positive for estrogen and/or progesterone receptor. HSP27 has also been implicated in drug resistance in cancer cells.
Research Area Heat Shock Proteins
Pathways MAPK Signaling

Application Details

Product Details anti-HSPB1 Antibody Target Details HSPB1 Handling Images back to top
Application Notes The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/million cells,IF: 0.5-1 μg/mL,WB: 0.25-0.5 μg/mL,IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2)

Restrictions For Research Use only

Handling

Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details Images back to top
Concentration 0.2 mg/mL
Buffer 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C,4 °C
Storage Comment Store the HSP27 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Images

Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-HSPB1 antibody (Heat Shock 27kDa Protein 1) (ABIN3026467) IHC testing of breast carcinoma (20X) stained with HSP27 antibody (G3.1).
Immunohistochemistry (IHC) image for anti-HSPB1 antibody (Heat Shock 27kDa Protein 1) (ABIN3026467) IHC testing of FFPE human prostate carcinoma and HSP27 antibody.
Immunohistochemistry (IHC) image for anti-HSPB1 antibody (Heat Shock 27kDa Protein 1) (ABIN3026467) IHC testing of human breast carcinoma (10X) stained with HSP27 antibody (G3.1).