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anti-Human JNK antibody for Enzyme Immunoassay

Recommended JNK Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase 8 (MAPK8) Antibodies
  • AI849689
  • Bsk
  • BSK
  • BSK/DJNK
  • c-Jun
  • CG5680
  • D-JNK
  • D-junk
  • DBSK/JNK
  • dJNK
  • DJNK
  • DJNK/bsk
  • Dmel\\CG5680
  • jnk
  • JNK
  • Jnk
  • JNK-46
  • JNK/SAPK
  • JNK1
  • jnk1
  • JNK1A2
  • JNK21B1/2
  • Junk
  • mapk8
  • Prkm8
  • PRKM8
  • sapk1
  • SAPK1
  • SAPK1c
  • SAPKa
  • T10F20.15
  • zgc:112379
Epitope
pThr183
95
58
33
22
18
15
15
15
13
13
13
10
10
10
10
5
5
5
5
4
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
374
133
113
17
15
7
6
4
2
2
2
1
1
Host
Rabbit
306
94
2
Clonality
Polyclonal
Conjugate
This JNK antibody is un-conjugated
18
16
14
11
11
10
4
3
3
3
3
3
3
3
3
Application
Enzyme Immunoassay (EIA)
300
195
92
65
53
53
39
33
16
15
10
6
4
3
2
2
1
1
1
1
1
1
Supplier
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Catalog No. ABIN358374
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008867729 ABIN953314 EIA IHC (p) WB Rabbit Ig AA 162-199 Log in to see Polyclonal 5
0.0008867729 ABIN359636 EIA WB Rabbit Ig C-Term Log in to see Polyclonal 5
0.0008867729 ABIN126826 EIA IHC (fro) WB Mouse IgG1 N-Term Log in to see 5D10
0.0008867729 ABIN126825 EIA IHC (fro) WB Biotin Mouse IgG1 N-Term Log in to see 5D10
0.0008867729 ABIN126827 EIA IHC (fro) IP WB Mouse IgG1 Log in to see 9H8
0.0008867729 ABIN953306 EIA IHC (p) IP WB Mouse IgG2a Log in to see 2F3
0.0008867729 ABIN953307 EIA IHC (p) IP WB Mouse IgG2a Log in to see 3H2
0.0008867729 ABIN1108135 EIA WB Mouse IgG1 Log in to see 1-00E-005
0.0008867729 ABIN953310 EIA IHC (p) WB Mouse IgG1 Log in to see 3B12
0.0008867729 ABIN953311 EIA IHC (p) WB Mouse IgG2b Log in to see 1-00E-002
0.0008867729 ABIN953309 EIA IHC (p) WB Mouse IgG2a Log in to see 1A2
0.0008867729 ABIN953313 EIA IHC (p) IP WB Mouse IgG2b Log in to see 4H5
0.0008867729 ABIN953308 EIA IHC (p) WB Mouse IgG2a Log in to see 1-00E-001
0.0008867729 ABIN953312 EIA IHC (p) IP WB Mouse IgG2a Log in to see 4H6
0.0008867729 ABIN2725439 EIA Rabbit IgG Log in to see Polyclonal
0.0008867729 ABIN641238 CA DB EIA IA ISt ELISA WB Rabbit Log in to see Polyclonal

Top referenced anti-JNK antibody for Enzyme Immunoassay

Similar anti-JNK Antibodies

Application / Reactivity Human
Biochemical Assay (BCA) 2 Antibodies
Cellular Assay (CA) 1 Antibodies
Dot Blot (DB) 39 Antibodies
ELISA 180 Antibodies
Enzyme Immunoassay (EIA) 17 Antibodies
Flow Cytometry (FACS) 10 Antibodies
Immunoassay (IA) 1 Antibodies
Immunochromatography (IC) 4 Antibodies
Immunocytochemistry (ICC) 14 Antibodies
Immunofluorescence (IF) 54 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 33 Antibodies
Immunohistochemistry (IHC) 91 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 4 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 65 Antibodies
Immunoprecipitation (IP) 47 Antibodies
Immunostaining (ISt) 1 Antibodies
Intracellular Staining (ICS) 2 Antibodies

General

Antigen Mitogen-Activated Protein Kinase 8 (MAPK8) Antibodies
Epitope pThr183
(95), (58), (33), (22), (18), (15), (15), (15), (13), (13), (13), (10), (10), (10), (10), (5), (5), (5), (5), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(374), (133), (113), (17), (15), (7), (6), (4), (2), (2), (2), (1), (1)
Host Rabbit
(306), (94), (2)
Clonality
Polyclonal
Conjugate This JNK antibody is un-conjugated
(18), (16), (14), (11), (11), (10), (4), (3), (3), (3), (3), (3), (3), (3), (3)
Application Enzyme Immunoassay (EIA)
(300), (195), (92), (65), (53), (53), (39), (33), (16), (15), (10), (6), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-JNK Antibody

Target Details JNK Application Details Handling References for anti-JNK Antibody (ABIN358374) Images
Specificity This antibody detects MAPK8 pThr183. Predicted to cross react with Mouse (100 % Antigen Homology).
Purification Protein A Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with peptides. The antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Thr183 of human MAPK8.
Isotype Ig

Target Details JNK

Product Details anti-JNK Antibody Application Details Handling References for anti-JNK Antibody (ABIN358374) Images back to top
Antigen
Alternative Name MAPK8 / JNK1 (MAPK8 Antibody Abstract)
Background MAPK8 is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation.Synonyms: JNK-1, JNK-46, Mitogen-activated protein kinase 8, PRKM8, Stress-activated protein kinase JNK1, c-Jun N-terminal kinase 1
Molecular Weight 48295 Da
Gene ID 5599, 9606
UniProt P45983
Pathways MAPK Signaling, WNT Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-JNK Antibody Target Details JNK Handling References for anti-JNK Antibody (ABIN358374) Images back to top
Application Notes ELISA: 1/1,000. Dot Blot: 1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-JNK Antibody Target Details JNK Application Details References for anti-JNK Antibody (ABIN358374) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-JNK Antibody (ABIN358374)

Product Details anti-JNK Antibody Target Details JNK Application Details Handling Images back to top
Background publications

Deng, Ren, Yang, Lin, Wu: "A JNK-dependent pathway is required for TNFalpha-induced apoptosis." in: Cell, Vol. 115, Issue 1, pp. 61-70, 2003

Luo, Ng, Jin, Ye, Han, Lin: "Axin utilizes distinct regions for competitive MEKK1 and MEKK4 binding and JNK activation." in: The Journal of biological chemistry, Vol. 278, Issue 39, pp. 37451-8, 2003

Ghosh: "Inhibition of arachidonate 5-lipoxygenase triggers prostate cancer cell death through rapid activation of c-Jun N-terminal kinase." in: Biochemical and biophysical research communications, Vol. 307, Issue 2, pp. 342-9, 2003

Utsugi, Dobashi, Ishizuka, Endou, Hamuro, Murata, Nakazawa, Mori: "c-Jun N-terminal kinase negatively regulates lipopolysaccharide-induced IL-12 production in human macrophages: role of mitogen-activated protein kinase in glutathione redox regulation of IL-12 production." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 171, Issue 2, pp. 628-35, 2003

Vivo, Liu, Broaddus: "c-Jun N-terminal kinase contributes to apoptotic synergy induced by tumor necrosis factor-related apoptosis-inducing ligand plus DNA damage in chemoresistant, p53 inactive mesothelioma cells." in: The Journal of biological chemistry, Vol. 278, Issue 28, pp. 25461-7, 2003

Images

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Supplier Images
 image for anti-JNK antibody (Mitogen-Activated Protein Kinase 8) (pThr183) (ABIN358374) anti-Mitogen-Activated Protein Kinase 8 (MAPK8) (pThr183) antibody