anti-Human JNK antibody for Intracellular Staining

Recommended JNK Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase 8 (MAPK8) Antibodies
  • BSK
  • BSK/DJNK
  • Bsk
  • CG5680
  • D-JNK
  • D-junk
  • DBSK/JNK
  • DJNK
  • DJNK/bsk
  • Dmel\\CG5680
  • JNK
  • JNK/SAPK
  • Jnk
  • Junk
  • SAPKa
  • c-Jun
  • dJNK
  • jnk
  • JNK-46
  • JNK1
  • JNK1A2
  • JNK21B1/2
  • PRKM8
  • SAPK1
  • SAPK1c
  • AI849689
  • Prkm8
  • jnk1
  • sapk1
  • T10F20.15
  • mapk8
  • zgc:112379
  • basket
  • mitogen-activated protein kinase 8
  • mitogen-activated protein kinase 8b
  • Protein JNK-1
  • bsk
  • MAPK8
  • Mapk8
  • mapk8
  • ATMPK8
  • mapk8b
  • jnk-1
Epitope
pThr183
109
64
29
17
15
15
14
13
10
10
10
10
10
9
8
8
7
7
6
5
5
5
5
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
419
200
169
23
19
13
8
8
6
4
3
3
3
2
2
2
2
1
1
Host
Mouse
319
115
3
2
Clonality
Monoclonal
Conjugate
This JNK antibody is conjugated to Alexa Fluor 647
21
19
15
13
11
11
4
4
3
3
3
3
3
3
3
3
3
2
1
1
1
1
1
1
1
1
1
1
Application
Intracellular Staining (ICS)
334
210
100
76
62
62
39
37
31
22
19
8
4
3
3
2
2
1
1
1
1
1
1
1
1
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Catalog No. ABIN1177076
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1177075 ICS PE Mouse IgG1, kappa pThr183 Log in to see N9-66 3

General

Antigen Mitogen-Activated Protein Kinase 8 (MAPK8) Antibodies
Epitope pThr183
(109), (64), (29), (17), (15), (15), (14), (13), (10), (10), (10), (10), (10), (9), (8), (8), (7), (7), (6), (5), (5), (5), (5), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(419), (200), (169), (23), (19), (13), (8), (8), (6), (4), (3), (3), (3), (2), (2), (2), (2), (1), (1)
Host Mouse
(319), (115), (3), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This JNK antibody is conjugated to Alexa Fluor 647
(21), (19), (15), (13), (11), (11), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Intracellular Staining (ICS)
(334), (210), (100), (76), (62), (62), (39), (37), (31), (22), (19), (8), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-JNK Antibody

Target Details JNK Application Details Handling References for anti-JNK antibody (ABIN1177076) Images
Brand BD Phosflow™
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Phosphorylated Human JNK Peptide
Clone N9-66
Isotype IgG1, kappa

Target Details JNK

Product Details anti-JNK Antibody Application Details Handling References for anti-JNK antibody (ABIN1177076) Images back to top
Antigen
Alternative Name JNK (MAPK8 Antibody Abstract)
Background The N9-66 monoclonal antibody specifically binds to JNK1 and JNK2 phosphorylated at the pT183/pY185 sites. c-Jun NH2-terminal Kinases (JNKs), also called Stress Activated Protein Kinases (SAPKs), are mitogen-activated protein kinases (MAPKs) with observed molecular weights of ~46 kDa (JNK1) and ~54 kDa (JNK2). Along with the p38 and ERK families, JNK represents one of three major classes of MAPKs. Complete activation of JNK requires the phosphorylation of both Thr183 and Tyr185 that are located in a Thr-X-Tyr motif. Phosphorylation of these residues is carried out by MKK4 and MKK7 that are phosphorylated and activated by MEKKs and MLKs in response to stress signals delivered through small GTPases of the Rho family. Once activated, JNK can translocate into the nucleus and regulate the expression of genes through phosphorylation of c-Jun, ATF-2, and other transcription factors. JNK plays a role in signal transduction in response to cytokines and various forms of environmental stress, such as endotoxins, UV irradiation, heat, and hyperosmolarity. JNK is critical to the regulation of cell growth, apoptosis, and the cellular response to stress, making it an important factor in tumorigenesis and adaptive immunity. During antibody development, the N9-66 monoclonal antibody was found to detect phosphorylated JNK1/2 by Western blot analysis of cellular lysates and by immunofluorescent staining and flow cytometric analysis of fixed and permeabilized cells. This antibody crossreacts with phosphorylated JNK1/2 expressed by mouse cells, as tested by Western blot analysis and flow cytometry.
Synonyms: MAPK8, MAPK9, SAPK1, SAPK, PRKM8, PRKM9
Pathways MAPK Signaling, WNT Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades, Signaling of Hepatocyte Growth Factor Receptor

Application Details

Product Details anti-JNK Antibody Target Details JNK Handling References for anti-JNK antibody (ABIN1177076) Images back to top
Sample Volume 5 μL
Restrictions For Research Use only

Handling

Product Details anti-JNK Antibody Target Details JNK Application Details References for anti-JNK antibody (ABIN1177076) Images back to top
Format Liquid
Buffer Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References for anti-JNK antibody (ABIN1177076)

Product Details anti-JNK Antibody Target Details JNK Application Details Handling Images back to top
Product cited in:

Huang, Shi, Chi: "Regulation of JNK and p38 MAPK in the immune system: signal integration, propagation and termination." in: Cytokine, Vol. 48, Issue 3, pp. 161-9, 2009

Wagner, Nebreda: "Signal integration by JNK and p38 MAPK pathways in cancer development." in: Nature reviews. Cancer, Vol. 9, Issue 8, pp. 537-49, 2009

Fleming, Armstrong, Morrice, Paterson, Goedert, Cohen: "Synergistic activation of stress-activated protein kinase 1/c-Jun N-terminal kinase (SAPK1/JNK) isoforms by mitogen-activated protein kinase kinase 4 (MKK4) and MKK7." in: The Biochemical journal, Vol. 352 Pt 1, Issue 3, pp. 145-54, 2001

Kyriakis, Avruch: "Mammalian mitogen-activated protein kinase signal transduction pathways activated by stress and inflammation." in: Physiological reviews, Vol. 81, Issue 2, pp. 807-69, 2001