anti-Human JNK antibody for Western Blotting

Recommended JNK Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase 8 (MAPK8) Antibodies
  • BSK
  • BSK/DJNK
  • Bsk
  • CG5680
  • D-JNK
  • D-junk
  • DBSK/JNK
  • DJNK
  • DJNK/bsk
  • Dmel\\CG5680
  • JNK
  • JNK/SAPK
  • Jnk
  • Junk
  • SAPKa
  • c-Jun
  • dJNK
  • jnk
  • JNK-46
  • JNK1
  • JNK1A2
  • JNK21B1/2
  • PRKM8
  • SAPK1
  • SAPK1c
  • AI849689
  • Prkm8
  • jnk1
  • sapk1
  • T10F20.15
  • mapk8
  • zgc:112379
  • basket
  • mitogen-activated protein kinase 8
  • mitogen-activated protein kinase 8b
  • Protein JNK-1
  • bsk
  • MAPK8
  • Mapk8
  • mapk8
  • ATMPK8
  • mapk8b
  • jnk-1
Epitope
AA 358-389, C-Term
110
64
28
17
15
15
14
13
10
10
10
10
10
9
8
8
7
7
5
5
5
5
5
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
419
201
169
23
19
13
8
8
6
4
3
3
3
2
2
2
2
1
1
Host
Rabbit
318
116
3
2
Clonality
Polyclonal
Conjugate
This JNK antibody is un-conjugated
21
19
15
13
11
11
5
4
3
3
3
3
3
3
3
3
3
2
1
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
333
210
100
75
62
61
39
37
31
22
19
8
4
3
3
2
2
2
1
1
1
1
1
1
1
Options
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Catalog No. ABIN391724
$ 324.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.1469584 ABIN4327961 IHC IHC (p) WB Rabbit pThr183 Log in to see Polyclonal 2
3.1469584 ABIN2706428 IC IF IHC WB Rabbit pSer183 Log in to see Polyclonal
3.1469584 ABIN953314 EIA IHC (p) WB Rabbit Ig AA 162-199 Log in to see Polyclonal 5
3.1469584 ABIN359636 EIA WB Rabbit Ig C-Term Log in to see Polyclonal 5
3.1469584 ABIN2452243 WB Mouse IgG2a AA 1-384 Log in to see 2F10 1
3.1469584 ABIN732368 IF (p) IHC (p) WB Rabbit IgG AA 170-210, pThr183, pTyr185 Log in to see Polyclonal 11
3.1469584 ABIN2957645 IC IF IHC WB Rabbit Log in to see Polyclonal
3.1469584 ABIN968866 FACS WB Mouse IgG1 pThr183 Log in to see 41-JNK-SAPK 4
3.1469584 ABIN968867 FACS WB Mouse IgG1 pThr183 Log in to see 41-JNK-SAPK 5
3.1469584 ABIN4327964 ICC IF IHC IHC (p) WB Rabbit Log in to see Polyclonal 1
3.1469584 ABIN1532353 IF ELISA WB Rabbit IgG AA 151-200 Log in to see Polyclonal
3.1469584 ABIN1531332 ELISA WB Rabbit IgG AA 151-200, pThr183 Log in to see Polyclonal
3.1469584 ABIN967330 IP WB Mouse IgG1 Log in to see G151-333 7
3.1469584 ABIN967329 IP WB Mouse IgG1 Log in to see G151-333 8
3.1469584 ABIN3182721 ELISA IHC WB Rabbit IgG pTyr185 Log in to see Polyclonal
3.1469584 ABIN3185268 ELISA IF IHC WB Rabbit IgG Lys25 Log in to see Polyclonal
3.1469584 ABIN3185269 ELISA IF IHC WB Rabbit IgG Lys27 Log in to see Polyclonal
3.1469584 ABIN3185270 ELISA IHC WB Rabbit IgG Lys278 Log in to see Polyclonal
3.1469584 ABIN3198169 IC IF IHC WB Rabbit Log in to see Polyclonal
3.1469584 ABIN3182056 ELISA WB Rabbit IgG pThr183, pTyr185 Log in to see Polyclonal

General

Antigen Mitogen-Activated Protein Kinase 8 (MAPK8) Antibodies
Epitope AA 358-389, C-Term
(110), (64), (28), (17), (15), (15), (14), (13), (10), (10), (10), (10), (10), (9), (8), (8), (7), (7), (5), (5), (5), (5), (5), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(419), (201), (169), (23), (19), (13), (8), (8), (6), (4), (3), (3), (3), (2), (2), (2), (2), (1), (1)
Host Rabbit
(318), (116), (3), (2)
Clonality (Clone)
Polyclonal   ( )
Conjugate This JNK antibody is un-conjugated
(21), (19), (15), (13), (11), (11), (5), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(333), (210), (100), (75), (62), (61), (39), (37), (31), (22), (19), (8), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 6 references available
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Product Details anti-JNK Antibody

Target Details JNK Application Details Handling References for anti-JNK antibody (ABIN391724) Images
Specificity This JNK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 366~396 amino acids from the C-terminal region of human JNK1.
Predicted Reactivity Rat (Rattus)
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Immunogen This MAPK8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 358-389 AA from the C-terminal region of human MAPK8.
Clone RB0922
Isotype Ig

Target Details JNK

Product Details anti-JNK Antibody Application Details Handling References for anti-JNK antibody (ABIN391724) Images back to top
Antigen
Alternative Name JNK1 (MAPK8 Antibody Abstract)
Background JNK1 is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation.
Synonyms: Mitogen-activated protein kinase 8, Stress-activated protein kinase JNK1, c-Jun N-terminal kinase 1, JNK-46
Molecular Weight 48295 DA
Gene ID 5599
UniProt P45983
Research Area Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cancer, Cell Cycle, Transcription Factors, Signaling, Cell Structure
Pathways MAPK Signaling, WNT Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades, Signaling of Hepatocyte Growth Factor Receptor

Application Details

Product Details anti-JNK Antibody Target Details JNK Handling References for anti-JNK antibody (ABIN391724) Images back to top
Application Notes WB = 1:1000, IF = 1:10-50, IHC (p) = 1:10-50
Restrictions For Research Use only

Handling

Product Details anti-JNK Antibody Target Details JNK Application Details References for anti-JNK antibody (ABIN391724) Images back to top
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months

References for anti-JNK antibody (ABIN391724)

Product Details anti-JNK Antibody Target Details JNK Application Details Handling Images back to top
Product cited in:

Li, Sahbaie, Zheng, Ritchie, Peltz, Mogil, Clark: "Expression genetics identifies spinal mechanisms supporting formalin late phase behaviors." in: Molecular pain, Vol. 6, pp. 11, 2010

Background publications

Deng, Ren, Yang, Lin, Wu: "A JNK-dependent pathway is required for TNFalpha-induced apoptosis." in: Cell, Vol. 115, Issue 1, pp. 61-70, 2003

Luo, Ng, Jin, Ye, Han, Lin: "Axin utilizes distinct regions for competitive MEKK1 and MEKK4 binding and JNK activation." in: The Journal of biological chemistry, Vol. 278, Issue 39, pp. 37451-8, 2003

Ghosh: "Inhibition of arachidonate 5-lipoxygenase triggers prostate cancer cell death through rapid activation of c-Jun N-terminal kinase." in: Biochemical and biophysical research communications, Vol. 307, Issue 2, pp. 342-9, 2003

Utsugi, Dobashi, Ishizuka, Endou, Hamuro, Murata, Nakazawa, Mori: "c-Jun N-terminal kinase negatively regulates lipopolysaccharide-induced IL-12 production in human macrophages: role of mitogen-activated protein kinase in glutathione redox regulation of IL-12 production." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 171, Issue 2, pp. 628-35, 2003

Vivo, Liu, Broaddus: "c-Jun N-terminal kinase contributes to apoptotic synergy induced by tumor necrosis factor-related apoptosis-inducing ligand plus DNA damage in chemoresistant, p53 inactive mesothelioma cells." in: The Journal of biological chemistry, Vol. 278, Issue 28, pp. 25461-7, 2003

Images

Product Details anti-JNK Antibody Target Details JNK Application Details Handling References for anti-JNK antibody (ABIN391724) back to top
Supplier Images
Western Blotting (WB) image for anti-Mitogen-Activated Protein Kinase 8 (MAPK8) (AA 358-389), (C-Term) antibody (ABIN391724) Western blot analysis of anti-JNK1 Pab (ABIN391724) in HL60 (UV-treated) cell lysate....