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anti-Human JNK antibody for Western Blotting

Recommended JNK Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase 8 (MAPK8) Antibodies
  • AI849689
  • Bsk
  • BSK
  • BSK/DJNK
  • c-Jun
  • CG5680
  • D-JNK
  • D-junk
  • DBSK/JNK
  • dJNK
  • DJNK
  • DJNK/bsk
  • Dmel\\CG5680
  • jnk
  • JNK
  • Jnk
  • JNK-46
  • JNK/SAPK
  • JNK1
  • jnk1
  • JNK1A2
  • JNK21B1/2
  • Junk
  • mapk8
  • Prkm8
  • PRKM8
  • sapk1
  • SAPK1
  • SAPK1c
  • SAPKa
  • T10F20.15
  • zgc:112379
Epitope
AA 358-389, C-Term
95
58
32
22
18
15
15
15
13
13
13
10
10
10
10
5
5
5
5
4
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
369
129
109
17
15
7
6
4
2
2
2
1
1
Host
Rabbit
303
92
2
Clonality
Polyclonal
Conjugate
This JNK antibody is un-conjugated
18
16
14
11
11
10
4
3
3
3
3
3
3
3
3
Application
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
294
194
89
61
52
49
39
33
17
12
9
6
4
3
2
2
1
1
1
1
1
1
Supplier
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Catalog No. ABIN391724
$ 324.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.12611513 ABIN953314 EIA IHC (p) WB Rabbit Ig AA 162-199 Log in to see Polyclonal 5
0.12611513 ABIN2452243 WB Mouse IgG2a AA 1-384 Log in to see 2F10 1
0.12611513 ABIN359636 EIA WB Rabbit Ig C-Term Log in to see Polyclonal 5
0.12611513 ABIN732368 IF (p) IHC (p) WB Rabbit IgG AA 170-210, pThr183, pTyr185 Log in to see Polyclonal 4
0.12611513 ABIN967329 IP WB Mouse IgG1 Log in to see G151-333 8
0.12611513 ABIN967330 IP WB Mouse IgG1 Log in to see G151-333 7
0.12611513 ABIN968866 FACS WB Mouse IgG1 pThr183 Log in to see 41-JNK-SAPK 4
0.12611513 ABIN968867 FACS WB Mouse IgG1 pThr183 Log in to see 41-JNK-SAPK 5
0.12611513 ABIN126827 EIA IHC (fro) IP WB Mouse IgG1 Log in to see 9H8
0.12611513 ABIN2452244 WB Mouse IgG2a AA 1-384 Log in to see 3B4
0.12611513 ABIN2452351 IF WB Mouse IgG2a AA 1-384 Log in to see 1C2
0.12611513 ABIN1531332 ELISA WB Rabbit IgG pThr183, pTyr185 Log in to see Polyclonal
0.12611513 ABIN2854959 ICC IF IHC (p) WB Rabbit IgG Center Log in to see Polyclonal
0.12611513 ABIN953306 EIA IHC (p) IP WB Mouse IgG2a Log in to see 2F3
0.12611513 ABIN953307 EIA IHC (p) IP WB Mouse IgG2a Log in to see 3H2
0.12611513 ABIN498747 IF IHC (p) WB Rabbit pThr183 Log in to see Polyclonal
0.12611513 ABIN1532353 IF ELISA WB Rabbit IgG Log in to see Polyclonal
0.12611513 ABIN3043004 WB Rabbit IgG AA 1-15, N-Term Log in to see Polyclonal
0.12611513 ABIN3042709 ICC IHC (p) WB Rabbit IgG AA 344-363, Middle Region Log in to see Polyclonal
0.12611513 ABIN498628 IHC (p) WB Rabbit Log in to see Polyclonal

Top referenced anti-JNK antibody for Western Blotting

Similar anti-JNK Antibodies

Application / Reactivity Human
Biochemical Assay (BCA) 2 Antibodies
Cellular Assay (CA) 1 Antibodies
Dot Blot (DB) 39 Antibodies
ELISA 179 Antibodies
Enzyme Immunoassay (EIA) 17 Antibodies
Flow Cytometry (FACS) 9 Antibodies
Immunoassay (IA) 1 Antibodies
Immunochromatography (IC) 4 Antibodies
Immunocytochemistry (ICC) 12 Antibodies
Immunofluorescence (IF) 52 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 33 Antibodies
Immunohistochemistry (IHC) 88 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 4 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 62 Antibodies
Immunoprecipitation (IP) 47 Antibodies
Immunostaining (ISt) 1 Antibodies
Intracellular Staining (ICS) 2 Antibodies

General

Antigen Mitogen-Activated Protein Kinase 8 (MAPK8) Antibodies
Epitope AA 358-389, C-Term
(95), (58), (32), (22), (18), (15), (15), (15), (13), (13), (13), (10), (10), (10), (10), (5), (5), (5), (5), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(369), (129), (109), (17), (15), (7), (6), (4), (2), (2), (2), (1), (1)
Host Rabbit
(303), (92), (2)
Clonality (Clone)
Polyclonal   ( )
Conjugate This JNK antibody is un-conjugated
(18), (16), (14), (11), (11), (10), (4), (3), (3), (3), (3), (3), (3), (3), (3)
Application Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(294), (194), (89), (61), (52), (49), (39), (33), (17), (12), (9), (6), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 6 references available
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Product Details anti-JNK Antibody

Target Details JNK Application Details Handling References for anti-JNK Antibody (ABIN391724) Images
Specificity This JNK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 366~396 amino acids from the C-terminal region of human JNK1.
Predicted Reactivity Rat (Rattus)
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Immunogen This MAPK8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 358-389 AA from the C-terminal region of human MAPK8.
Clone RB0922
Isotype Ig

Target Details JNK

Product Details anti-JNK Antibody Application Details Handling References for anti-JNK Antibody (ABIN391724) Images back to top
Antigen
Alternative Name JNK1 (MAPK8 Antibody Abstract)
Background JNK1 is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation.
Synonyms: Mitogen-activated protein kinase 8, Stress-activated protein kinase JNK1, c-Jun N-terminal kinase 1, JNK-46
Molecular Weight 48295 DA
Gene ID 5599
UniProt P45983
Research Area Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cancer, Cell Cycle, Transcription Factors, Signaling, Cell Structure
Pathways MAPK Signaling, WNT Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-JNK Antibody Target Details JNK Handling References for anti-JNK Antibody (ABIN391724) Images back to top
Application Notes WB = 1:1000, IF = 1:10-50, IHC (p) = 1:10-50
Restrictions For Research Use only

Handling

Product Details anti-JNK Antibody Target Details JNK Application Details References for anti-JNK Antibody (ABIN391724) Images back to top
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months

References for anti-JNK Antibody (ABIN391724)

Product Details anti-JNK Antibody Target Details JNK Application Details Handling Images back to top
Product cited in:

Li, Sahbaie, Zheng, Ritchie, Peltz, Mogil, Clark: "Expression genetics identifies spinal mechanisms supporting formalin late phase behaviors." in: Molecular pain, Vol. 6, pp. 11, 2010

Background publications

Deng, Ren, Yang, Lin, Wu: "A JNK-dependent pathway is required for TNFalpha-induced apoptosis." in: Cell, Vol. 115, Issue 1, pp. 61-70, 2003

Luo, Ng, Jin, Ye, Han, Lin: "Axin utilizes distinct regions for competitive MEKK1 and MEKK4 binding and JNK activation." in: The Journal of biological chemistry, Vol. 278, Issue 39, pp. 37451-8, 2003

Ghosh: "Inhibition of arachidonate 5-lipoxygenase triggers prostate cancer cell death through rapid activation of c-Jun N-terminal kinase." in: Biochemical and biophysical research communications, Vol. 307, Issue 2, pp. 342-9, 2003

Utsugi, Dobashi, Ishizuka, Endou, Hamuro, Murata, Nakazawa, Mori: "c-Jun N-terminal kinase negatively regulates lipopolysaccharide-induced IL-12 production in human macrophages: role of mitogen-activated protein kinase in glutathione redox regulation of IL-12 production." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 171, Issue 2, pp. 628-35, 2003

Vivo, Liu, Broaddus: "c-Jun N-terminal kinase contributes to apoptotic synergy induced by tumor necrosis factor-related apoptosis-inducing ligand plus DNA damage in chemoresistant, p53 inactive mesothelioma cells." in: The Journal of biological chemistry, Vol. 278, Issue 28, pp. 25461-7, 2003

Images

Product Details anti-JNK Antibody Target Details JNK Application Details Handling References for anti-JNK Antibody (ABIN391724) back to top
Supplier Images
Western Blotting (WB) image for anti-JNK antibody (Mitogen-Activated Protein Kinase 8) (AA 358-389) (ABIN391724) Western blot analysis of anti-JNK1 Pab (ABIN391724) in HL60 (UV-treated) cell lysate....