We are preparing the requested document. Please wait, this may take a while...!
|Application / Reactivity||Human|
|Biochemical Assay (BCA)||2 Antibodies|
|Cellular Assay (CA)||1 Antibodies|
|Dot Blot (DB)||39 Antibodies|
|Enzyme Immunoassay (EIA)||17 Antibodies|
|Flow Cytometry (FACS)||9 Antibodies|
|Immunoassay (IA)||1 Antibodies|
|Immunochromatography (IC)||4 Antibodies|
|Immunocytochemistry (ICC)||12 Antibodies|
|Immunofluorescence (IF)||52 Antibodies|
|Immunofluorescence (Cultured Cells) (IF (cc))||1 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||33 Antibodies|
|Immunohistochemistry (IHC)||88 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||4 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||62 Antibodies|
|Immunoprecipitation (IP)||47 Antibodies|
|Immunostaining (ISt)||1 Antibodies|
|Intracellular Staining (ICS)||2 Antibodies|
|Antigen||Mitogen-Activated Protein Kinase 8 (MAPK8) Antibodies|
|Epitope||AA 358-389, C-Term Alternatives|
|Conjugate||This JNK antibody is un-conjugated Alternatives|
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|6 references available|
|Supplier||Log in to see|
Product Details anti-JNK AntibodyTarget Details JNK Application Details Handling References for anti-JNK Antibody (ABIN391724) Images
|Specificity||This JNK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 366~396 amino acids from the C-terminal region of human JNK1.|
|Predicted Reactivity||Rat (Rattus)|
|Purification||This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Immunogen||This MAPK8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 358-389 AA from the C-terminal region of human MAPK8.|
Target Details JNKProduct Details anti-JNK Antibody Application Details Handling References for anti-JNK Antibody (ABIN391724) Images back to top
|Alternative Name||JNK1 (MAPK8 Antibody Abstract)|
JNK1 is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation.
Synonyms: Mitogen-activated protein kinase 8, Stress-activated protein kinase JNK1, c-Jun N-terminal kinase 1, JNK-46
|Molecular Weight||48295 DA|
|Research Area||Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cancer, Cell Cycle, Transcription Factors, Signaling, Cell Structure|
|Pathways||MAPK Signaling, WNT Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway|
Application DetailsProduct Details anti-JNK Antibody Target Details JNK Handling References for anti-JNK Antibody (ABIN391724) Images back to top
|Application Notes||WB = 1:1000, IF = 1:10-50, IHC (p) = 1:10-50|
|Restrictions||For Research Use only|
HandlingProduct Details anti-JNK Antibody Target Details JNK Application Details References for anti-JNK Antibody (ABIN391724) Images back to top
|Buffer||PBS with 0.09 % (W/V) sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage||4 °C/-20 °C|
|Storage Comment||Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.|
|Expiry Date||6 months|
References for anti-JNK Antibody (ABIN391724)Product Details anti-JNK Antibody Target Details JNK Application Details Handling Images back to top
|Product cited in:||
Li, Sahbaie, Zheng et al.: "Expression genetics identifies spinal mechanisms supporting formalin late phase behaviors." in: Molecular pain, Vol. 6, pp. 11, 2010 (PubMed).
Deng, Ren, Yang et al.: "A JNK-dependent pathway is required for TNFalpha-induced apoptosis." in: Cell, Vol. 115, Issue 1, pp. 61-70, 2003 (PubMed).
Luo, Ng, Jin et al.: "Axin utilizes distinct regions for competitive MEKK1 and MEKK4 binding and JNK activation." in: The Journal of biological chemistry, Vol. 278, Issue 39, pp. 37451-8, 2003 (PubMed).
Ghosh: "Inhibition of arachidonate 5-lipoxygenase triggers prostate cancer cell death through rapid activation of c-Jun N-terminal kinase." in: Biochemical and biophysical research communications, Vol. 307, Issue 2, pp. 342-9, 2003 (PubMed).
Utsugi, Dobashi, Ishizuka et al.: "c-Jun N-terminal kinase negatively regulates lipopolysaccharide-induced IL-12 production in human macrophages: role of mitogen-activated protein kinase in glutathione redox regulation of IL-12 production." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 171, Issue 2, pp. 628-35, 2003 (PubMed).
Vivo, Liu, Broaddus: "c-Jun N-terminal kinase contributes to apoptotic synergy induced by tumor necrosis factor-related apoptosis-inducing ligand plus DNA damage in chemoresistant, p53 inactive mesothelioma cells." in: The Journal of biological chemistry, Vol. 278, Issue 28, pp. 25461-7, 2003 (PubMed).
ImagesProduct Details anti-JNK Antibody Target Details JNK Application Details Handling References for anti-JNK Antibody (ABIN391724) back to top