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|Application / Reactivity||Rat (Rattus)||Mouse (Murine)|
|ELISA||30 Antibodies||38 Antibodies|
|Enzyme Immunoassay (EIA)||2 Antibodies||5 Antibodies|
|Flow Cytometry (FACS)||6 Antibodies||9 Antibodies|
|Functional Studies (Func)||1 Antibodies|
|Immunochromatography (IC)||3 Antibodies||3 Antibodies|
|Immunocytochemistry (ICC)||7 Antibodies||8 Antibodies|
|Immunofluorescence (IF)||20 Antibodies||23 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||33 Antibodies||33 Antibodies|
|Immunohistochemistry (IHC)||27 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||3 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||19 Antibodies|
|Immunoprecipitation (IP)||7 Antibodies|
|Western Blotting (WB)||76 Antibodies|
|Antigen||Mitogen-Activated Protein Kinase 8 (MAPK8) Antibodies|
|Epitope||AA 344-363, Middle Region Alternatives|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This JNK antibody is un-conjugated Alternatives|
Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-JNK AntibodyTarget Details JNK Application Details Handling Images
|Cross-Reactivity (Details)||No cross reactivity with other proteins.|
Rabbit IgG polyclonal antibody for Mitogen-activated protein kinase 8(MAPK8) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.
Gene Name: Mitogen-activated Protein Kinase 8
Protein Name: Mitogen-activated protein kinase 8(MAP kinase 8/MAPK 8)
|Purification||Immunogen affinity purified.|
|Immunogen||A synthetic peptide corresponding to a sequence in the middle region of human MAPK8/9(344-363aa EREHTIEEWKELIYKEVMDL), identical to the related mouse and rat sequences.|
Target Details JNKProduct Details anti-JNK Antibody Application Details Handling Images back to top
|Alternative Name||MAPK8 (MAPK8 Antibody Abstract)|
|Background||Mitogen-activated protein kinase 8 is an enzyme that in humans is encoded by the MAPK8 gene. The protein encoded by this gene is a member of the MAP kinase family. The International Radiation Hybrid Mapping Consortium mapped the MAPK8 gene to chromosome 10. The single long open reading frame encodes a putative protein kinase with a predicted mass of 44.2 kD. This kinase play a key role in T cell proliferation, apoptosis and differentiation.|
|Pathways||MAPK Signaling, WNT Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway|
Application DetailsProduct Details anti-JNK Antibody Target Details JNK Handling Images back to top
WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.
|Restrictions||For Research Use only|
HandlingProduct Details anti-JNK Antibody Target Details JNK Application Details Images back to top
|Reconstitution||Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.|
|Buffer||Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.|
|Preservative||Thimerosal (Merthiolate), Sodium azide|
|Precaution of Use||This product contains Thimerosal (Merthiolate),Sodium azide a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||-20 °C,4 °C|
At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
|Expiry Date||12 months|
ImagesProduct Details anti-JNK Antibody Target Details JNK Application Details Handling back to top