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|Antigen||Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) Antibodies|
|Conjugate||This MAP2K1 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA)
|4 references available|
|Supplier||Log in to see|
Product Details anti-MAP2K1 AntibodyTarget Details MAP2K1 Application Details Handling References for anti-MAP2K1 antibody (ABIN358538) Images
|Specificity||This antibody reacts to MEK1 pSer222.|
|Purification||Protein A column, followed by two-step phosphospecific peptide affinity purification|
|Immunogen||This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S222 of human MEK1.|
Target Details MAP2K1Product Details anti-MAP2K1 Antibody Application Details Handling References for anti-MAP2K1 antibody (ABIN358538) Images back to top
|Alternative Name||MAP2K1 (MAP2K1 Antibody Abstract)|
|Background||MEK1is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is known to play a critical role in mitogen growth factor signal transduction. It phosphorylates and thus activates MAPK1/ERK2 and MAPK2/ERK3. The activation of this kinase itself is dependent on the Ser/Thr phosphorylation by MAP kinase kinase kinases. The inhibition or degradation of this kinase is found to be involved in the pathogenesis of Yersinia and anthrax.Synonyms: Dual specificity mitogen-activated protein kinase kinase 1, ERK activator kinase 1, MAP kinase kinase 1, MAPK/ERK kinase 1, MAPKK 1, MEK1, PRKMK1|
|Gene ID||5604, 9606|
|Research Area||Kinases/Phosphatases, Phospho-specific antibodies|
|Pathways||MAPK Signaling, RTK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Autophagy, Signaling of Hepatocyte Growth Factor Receptor|
Application DetailsProduct Details anti-MAP2K1 Antibody Target Details MAP2K1 Handling References for anti-MAP2K1 antibody (ABIN358538) Images back to top
ELISA: 1/1,000. Dot Blot: 1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-MAP2K1 Antibody Target Details MAP2K1 Application Details References for anti-MAP2K1 antibody (ABIN358538) Images back to top
|Buffer||PBS with 0.09 % (W/V) sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.|
References for anti-MAP2K1 antibody (ABIN358538)Product Details anti-MAP2K1 Antibody Target Details MAP2K1 Application Details Handling Images back to top
Ohren, Chen, Pavlovsky, Whitehead, Zhang, Kuffa, Yan, McConnell, Spessard, Banotai, Mueller, Delaney, Omer, Sebolt-Leopold, Dudley, Leung, Flamme, Warmus, Kaufman, Barrett, Tecle, Hasemann: "Structures of human MAP kinase kinase 1 (MEK1) and MEK2 describe novel noncompetitive kinase inhibition." in: Nature structural & molecular biology, Vol. 11, Issue 12, pp. 1192-7, 2004
Naegele, Morley: "Molecular cross-talk between MEK1/2 and mTOR signaling during recovery of 293 cells from hypertonic stress." in: The Journal of biological chemistry, Vol. 279, Issue 44, pp. 46023-34, 2004
Ussar, Voss: "MEK1 and MEK2, different regulators of the G1/S transition." in: The Journal of biological chemistry, Vol. 279, Issue 42, pp. 43861-9, 2004
Beausoleil, Jedrychowski, Schwartz, Elias, Villén, Li, Cohn, Cantley, Gygi: "Large-scale characterization of HeLa cell nuclear phosphoproteins." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 33, pp. 12130-5, 2004
ImagesProduct Details anti-MAP2K1 Antibody Target Details MAP2K1 Application Details Handling References for anti-MAP2K1 antibody (ABIN358538) back to top