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anti-Human MAP2K1 antibody for Enzyme Immunoassay

Recommended MAP2K1 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) Antibodies
  • ATMEK1
  • CFC3
  • F20B18.180
  • F20B18_180
  • MAP kinase/ ERK kinase 1
  • MAPKK1
  • MEK
  • mek-2
  • MEK1
  • Mek1
  • MEKK1
  • MITOGEN ACTIVATED PROTEIN KINASE KINASE
  • MITOGEN ACTIVATED PROTEIN KINASE KINASE 1
  • MKK1
  • NMAPKK
  • PRKMK1
  • Prkmk1
  • si:ch211-242m18.2
  • wu:fj56a12
  • wu:fj61b01
  • zgc:56557
Epitope
pSer222
43
38
34
32
32
32
30
27
25
22
21
15
15
13
13
13
11
9
7
7
6
6
6
6
5
4
4
4
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
606
324
281
60
38
24
15
14
13
6
4
4
4
2
2
Host
Rabbit
494
123
2
2
1
Clonality
Polyclonal
Conjugate
This MAP2K1 antibody is un-conjugated
14
14
14
11
9
8
8
8
6
6
6
6
6
6
6
1
1
Application
Enzyme Immunoassay (EIA)
455
208
157
116
95
66
23
22
14
10
9
6
5
2
2
2
2
2
1
Supplier
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Catalog No. ABIN358538
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008867729 ABIN360367 EIA WB Rabbit Ig Ser216 Log in to see Polyclonal 2
0.0008867729 ABIN358157 EIA IHC (p) WB Rabbit Ig pSer217, pSer221 Log in to see Polyclonal 5
0.0008867729 ABIN126836 EIA WB Mouse IgG1 pSer218, pSer222, pSer226 Log in to see 7-00E-010
0.0008867729 ABIN126835 EIA WB Mouse IgG1 Log in to see 10B1
0.0008867729 ABIN126837 EIA WB Mouse IgG2a Log in to see 9G3

Top referenced anti-MAP2K1 antibody for Enzyme Immunoassay

Similar anti-MAP2K1 Antibodies

Application / Reactivity Human
BioImaging (BI) 2 Antibodies
Dot Blot (DB) 23 Antibodies
ELISA 154 Antibodies
Enzyme Immunoassay (EIA) 6 Antibodies
Flow Cytometry (FACS) 3 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 15 Antibodies
Immunodiffusion (ID) 6 Antibodies
Immunofluorescence (IF) 117 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 66 Antibodies
Immunohistochemistry (IHC) 209 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 96 Antibodies
Immunoprecipitation (IP) 14 Antibodies
Intracellular Staining (ICS) 9 Antibodies
Luminex Assay (LMNX) 10 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Western Blotting (WB) 442 Antibodies

General

Antigen Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) Antibodies
Epitope pSer222
(43), (38), (34), (32), (32), (32), (30), (27), (25), (22), (21), (15), (15), (13), (13), (13), (11), (9), (7), (7), (6), (6), (6), (6), (5), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(606), (324), (281), (60), (38), (24), (15), (14), (13), (6), (4), (4), (4), (2), (2)
Host Rabbit
(494), (123), (2), (2), (1)
Clonality
Polyclonal
Conjugate This MAP2K1 antibody is un-conjugated
(14), (14), (14), (11), (9), (8), (8), (8), (6), (6), (6), (6), (6), (6), (6), (1), (1)
Application Enzyme Immunoassay (EIA)
(455), (208), (157), (116), (95), (66), (23), (22), (14), (10), (9), (6), (5), (2), (2), (2), (2), (2), (1)
Pubmed 4 references available
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Product Details anti-MAP2K1 Antibody

Target Details MAP2K1 Application Details Handling References for anti-MAP2K1 Antibody (ABIN358538) Images
Specificity This antibody reacts to MEK1 pSer222.
Purification Protein A column, followed by two-step phosphospecific peptide affinity purification
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S222 of human MEK1.
Isotype Ig

Target Details MAP2K1

Product Details anti-MAP2K1 Antibody Application Details Handling References for anti-MAP2K1 Antibody (ABIN358538) Images back to top
Antigen
Alternative Name MAP2K1 (MAP2K1 Antibody Abstract)
Background MEK1is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is known to play a critical role in mitogen growth factor signal transduction. It phosphorylates and thus activates MAPK1/ERK2 and MAPK2/ERK3. The activation of this kinase itself is dependent on the Ser/Thr phosphorylation by MAP kinase kinase kinases. The inhibition or degradation of this kinase is found to be involved in the pathogenesis of Yersinia and anthrax.Synonyms: Dual specificity mitogen-activated protein kinase kinase 1, ERK activator kinase 1, MAP kinase kinase 1, MAPK/ERK kinase 1, MAPKK 1, MEK1, PRKMK1
Gene ID 5604, 9606
UniProt Q02750
Research Area Kinases/Phosphatases, Phospho-specific antibodies
Pathways MAPK Signaling, RTK Signaling, TCR Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-MAP2K1 Antibody Target Details MAP2K1 Handling References for anti-MAP2K1 Antibody (ABIN358538) Images back to top
Application Notes ELISA: 1/1,000. Dot Blot: 1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-MAP2K1 Antibody Target Details MAP2K1 Application Details References for anti-MAP2K1 Antibody (ABIN358538) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-MAP2K1 Antibody (ABIN358538)

Product Details anti-MAP2K1 Antibody Target Details MAP2K1 Application Details Handling Images back to top
Background publications

Ohren, Chen, Pavlovsky, Whitehead, Zhang, Kuffa, Yan, McConnell, Spessard, Banotai, Mueller, Delaney, Omer, Sebolt-Leopold, Dudley, Leung, Flamme, Warmus, Kaufman, Barrett, Tecle, Hasemann: "Structures of human MAP kinase kinase 1 (MEK1) and MEK2 describe novel noncompetitive kinase inhibition." in: Nature structural & molecular biology, Vol. 11, Issue 12, pp. 1192-7, 2004

Naegele, Morley: "Molecular cross-talk between MEK1/2 and mTOR signaling during recovery of 293 cells from hypertonic stress." in: The Journal of biological chemistry, Vol. 279, Issue 44, pp. 46023-34, 2004

Ussar, Voss: "MEK1 and MEK2, different regulators of the G1/S transition." in: The Journal of biological chemistry, Vol. 279, Issue 42, pp. 43861-9, 2004

Beausoleil, Jedrychowski, Schwartz, Elias, Villén, Li, Cohn, Cantley, Gygi: "Large-scale characterization of HeLa cell nuclear phosphoproteins." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 33, pp. 12130-5, 2004

Images

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Supplier Images
 image for anti-MAP2K1 antibody (Mitogen-Activated Protein Kinase Kinase 1) (pSer222) (ABIN358538) anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (pSer222) antibody