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|Application / Reactivity||Human|
|BioImaging (BI)||2 Antibodies|
|Dot Blot (DB)||23 Antibodies|
|Enzyme Immunoassay (EIA)||6 Antibodies|
|Flow Cytometry (FACS)||3 Antibodies|
|Immunochromatography (IC)||2 Antibodies|
|Immunocytochemistry (ICC)||12 Antibodies|
|Immunodiffusion (ID)||6 Antibodies|
|Immunofluorescence (IF)||114 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||66 Antibodies|
|Immunohistochemistry (IHC)||201 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||3 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||88 Antibodies|
|Immunoprecipitation (IP)||13 Antibodies|
|Intracellular Staining (ICS)||9 Antibodies|
|Luminex Assay (LMNX)||10 Antibodies|
|Proximity Ligation Assay (PLA)||2 Antibodies|
|Western Blotting (WB)||416 Antibodies|
|Antigen||Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) Antibodies|
|Conjugate||This MAP2K1 antibody is un-conjugated Alternatives|
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
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Product Details anti-MAP2K1 AntibodyTarget Details MAP2K1 Application Details Handling Images
|Specificity||The antibody does not cross-react with other MAP kinase kinase family members.|
|No Cross-Reactivity||Mouse (Murine), Rat (Rattus)|
|Purification||Tissue culture supernatant|
|Immunogen||A synthetic peptide corresponding to residues in C-terminus of human MEK1 was used as immunogen.|
Target Details MAP2K1Product Details anti-MAP2K1 Antibody Application Details Handling Images back to top
|Alternative Name||MAP2K1 (MAP2K1 Antibody Abstract)|
MEK1 and MEK2 (MAPK kinase 1/2, or ERK kinase 1/2) are mitogen-activated proteinkinases that stimulate MAP kinase activity, playing a role in both cell growth anddifferentiation (1,2). MEK itself is activated via phosphorylation at serines 217/218 and221/222 by upstream activator kinases, including c-raf, mos and MEK kinase (3,4).MEK1 and MEK2 are activated by a wide variety of growth factors and cytokines, andalso by membrane depolarization and calcium influx (5). Alternate Names: anti-Dual specificity mitogen activated anti-protein kinase kinase 1 antibody, anti-ERKactivator kinase 1 antibody, anti-MAP kinase kinase 1 antibody, anti-MAP kinase/Erkkinase 1 antibody, anti-MAP/Erk kinase 1 antibody, anti-Map2K1 antibody, anti-MAPKK1 antibody, anti-MAPKK1 antibody, anti-MEK 1 antibody, anti-MEKK1 antibody,anti-Mitogen activated protein kinase kinase 1 antibody, anti-MKK 1 antibody,anti-MKK1 antibody, anti-PRKMK 1 antibody, anti-PRKMK1 antibody, anti-Proteinkinase mitogen activated kinase 1 antibody.
Gene Symbol: MAP2K1
|Gene ID||5604, 26395, 170851|
|Pathways||MAPK Signaling, RTK Signaling, TCR Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway|
Application DetailsProduct Details anti-MAP2K1 Antibody Target Details MAP2K1 Handling Images back to top
This product is useful for: Western Blot, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunoprecipitation, Flow Cytometry. In Western blot this antibody detects a band at approximately 45kDa.
Recommended dilutions: Flow Cytometry 1:40, Immunocytochemistry/Immunofluorescence 1:250-1:500, Immunohistochemistry 1:10-1:2000, Immunohistochemistry-Paraffin 1:250, Immunoprecipitation 1:50, Western Blot 1:1000-10000
Protocol specific for MEK1 Antibody Immunohistochemistry Protocol for Paraffin-embedded Tissues
1. Solutions and reagents1.
. Ethanol, anhydrous denatured, histological grade (100 %, 95 %, 70 %)1.
. Washing buffer:TBST washing buffer: 1XTBS/0.1 % Tween-20To prepare stock solution of 10X TBS: add 24.2 g Trizma base and 80 g sodium chloride to 1L of dH2O. Adjust pH to 7.6.Working solution. 1XTBST/0.1 % Tween-20: add 100ml 10XTBS to 900 mL dH2O. Add 1 mL Tween-20 and mix well.1.
. Distilled water (dH2O)1.
. Antigen Retrieval Solution:0.01M Sodium Citrate
|Restrictions||For Research Use only|
HandlingProduct Details anti-MAP2K1 Antibody Target Details MAP2K1 Application Details Images back to top
|Buffer||PBS 49 % , Glycerol 50 % , BSA 0.05 % , Sodium Azide|
|Precaution of Use||WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.|
|Handling Advice||Avoid freeze-thaw cycles|
|Storage Comment||-20 °C.|
ImagesProduct Details anti-MAP2K1 Antibody Target Details MAP2K1 Application Details Handling back to top