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anti-Human MAP2K1 antibody for Immunocytochemistry

Recommended MAP2K1 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) Antibodies
  • ATMEK1
  • CFC3
  • F20B18.180
  • F20B18_180
  • MAP kinase/ ERK kinase 1
  • MAPKK1
  • MEK
  • mek-2
  • MEK1
  • Mek1
  • MEKK1
  • MITOGEN ACTIVATED PROTEIN KINASE KINASE
  • MITOGEN ACTIVATED PROTEIN KINASE KINASE 1
  • MKK1
  • NMAPKK
  • PRKMK1
  • Prkmk1
  • si:ch211-242m18.2
  • wu:fj56a12
  • wu:fj61b01
  • zgc:56557
Epitope
C-Term
43
37
32
32
32
31
29
27
25
22
18
15
15
13
13
13
11
9
7
7
6
6
6
6
5
4
4
4
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
587
312
268
56
38
20
14
13
10
6
4
2
2
Host
Rabbit
477
120
2
2
1
Clonality
Monoclonal
Conjugate
This MAP2K1 antibody is un-conjugated
14
14
14
11
9
8
8
8
6
6
6
6
6
6
6
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
434
200
156
113
87
66
23
21
11
10
9
6
6
2
2
2
2
2
Supplier
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Catalog No. ABIN189514
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010161072 ABIN3044391 ICC IHC (p) WB Rabbit IgG AA 353-367, C-Term Log in to see Polyclonal
0.0010161072 ABIN2855379 ICC IF WB Rabbit IgG Center Log in to see Polyclonal
0.0010161072 ABIN1859743 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 54-369 Log in to see Polyclonal
0.0010161072 ABIN1493434 ICC IHC (p) WB Rabbit AA 353-367 Log in to see Polyclonal
0.0010161072 ABIN2586959 ICC Rabbit pThr292 Log in to see Polyclonal
0.0010161072 ABIN966512 ICC IHC (p) WB C-Term, AA 353-367 Log in to see Polyclonal 2
0.0010161072 ABIN2448577 ICC IHC (p) WB Rabbit C-Term Log in to see Polyclonal
0.0010161072 ABIN1825708 ICC WB Rabbit Log in to see Polyclonal
0.0010161072 ABIN2987553 ICC IF IHC WB Rabbit IgG pSer221 Log in to see Polyclonal
0.0010161072 ABIN2140366 ICC IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal
0.0010161072 ABIN3031839 ICC IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal

Top referenced anti-MAP2K1 antibody for Immunocytochemistry

Similar anti-MAP2K1 Antibodies

Application / Reactivity Human
BioImaging (BI) 2 Antibodies
Dot Blot (DB) 23 Antibodies
ELISA 153 Antibodies
Enzyme Immunoassay (EIA) 6 Antibodies
Flow Cytometry (FACS) 3 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 12 Antibodies
Immunodiffusion (ID) 6 Antibodies
Immunofluorescence (IF) 114 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 66 Antibodies
Immunohistochemistry (IHC) 201 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 88 Antibodies
Immunoprecipitation (IP) 13 Antibodies
Intracellular Staining (ICS) 9 Antibodies
Luminex Assay (LMNX) 10 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Western Blotting (WB) 422 Antibodies

General

Antigen Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) Antibodies
Epitope C-Term
(43), (37), (32), (32), (32), (31), (29), (27), (25), (22), (18), (15), (15), (13), (13), (13), (11), (9), (7), (7), (6), (6), (6), (6), (5), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(587), (312), (268), (56), (38), (20), (14), (13), (10), (6), (4), (2), (2)
Host Rabbit
(477), (120), (2), (2), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This MAP2K1 antibody is un-conjugated
(14), (14), (14), (11), (9), (8), (8), (8), (6), (6), (6), (6), (6), (6), (6), (1), (1)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(434), (200), (156), (113), (87), (66), (23), (21), (11), (10), (9), (6), (6), (2), (2), (2), (2), (2)
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Product Details anti-MAP2K1 Antibody

Target Details MAP2K1 Application Details Handling Images
Specificity The antibody does not cross-react with other MAP kinase kinase family members.
No Cross-Reactivity Mouse (Murine), Rat (Rattus)
Purification Tissue culture supernatant
Immunogen A synthetic peptide corresponding to residues in C-terminus of human MEK1 was used as immunogen.
Clone Y77
Isotype IgG

Target Details MAP2K1

Product Details anti-MAP2K1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name MAP2K1 (MAP2K1 Antibody Abstract)
Background MEK1 and MEK2 (MAPK kinase 1/2, or ERK kinase 1/2) are mitogen-activated proteinkinases that stimulate MAP kinase activity, playing a role in both cell growth anddifferentiation (1,2). MEK itself is activated via phosphorylation at serines 217/218 and221/222 by upstream activator kinases, including c-raf, mos and MEK kinase (3,4).MEK1 and MEK2 are activated by a wide variety of growth factors and cytokines, andalso by membrane depolarization and calcium influx (5). Alternate Names: anti-Dual specificity mitogen activated anti-protein kinase kinase 1 antibody, anti-ERKactivator kinase 1 antibody, anti-MAP kinase kinase 1 antibody, anti-MAP kinase/Erkkinase 1 antibody, anti-MAP/Erk kinase 1 antibody, anti-Map2K1 antibody, anti-MAPKK1 antibody, anti-MAPKK1 antibody, anti-MEK 1 antibody, anti-MEKK1 antibody,anti-Mitogen activated protein kinase kinase 1 antibody, anti-MKK 1 antibody,anti-MKK1 antibody, anti-PRKMK 1 antibody, anti-PRKMK1 antibody, anti-Proteinkinase mitogen activated kinase 1 antibody.
Gene Symbol: MAP2K1
Gene ID 5604, 26395, 170851
Pathways MAPK Signaling, RTK Signaling, TCR Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-MAP2K1 Antibody Target Details MAP2K1 Handling Images back to top
Application Notes This product is useful for: Western Blot, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunoprecipitation, Flow Cytometry. In Western blot this antibody detects a band at approximately 45kDa.
Recommended dilutions: Flow Cytometry 1:40, Immunocytochemistry/Immunofluorescence 1:250-1:500, Immunohistochemistry 1:10-1:2000, Immunohistochemistry-Paraffin 1:250, Immunoprecipitation 1:50, Western Blot 1:1000-10000
Protocol Protocol specific for MEK1 Antibody Immunohistochemistry Protocol for Paraffin-embedded Tissues
1. Solutions and reagents1.
. Xylene1.
. Ethanol, anhydrous denatured, histological grade (100 %, 95 %, 70 %)1.
. Washing buffer:TBST washing buffer: 1XTBS/0.1 % Tween-20To prepare stock solution of 10X TBS: add 24.2 g Trizma base and 80 g sodium chloride to 1L of dH2O. Adjust pH to 7.6.Working solution. 1XTBST/0.1 % Tween-20: add 100ml 10XTBS to 900 mL dH2O. Add 1 mL Tween-20 and mix well.1.
. Distilled water (dH2O)1.
. Antigen Retrieval Solution:0.01M Sodium Citrate
Restrictions For Research Use only

Handling

Product Details anti-MAP2K1 Antibody Target Details MAP2K1 Application Details Images back to top
Format Liquid
Buffer PBS 49 % , Glycerol 50 % , BSA 0.05 % , Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment -20 °C.

Images

Product Details anti-MAP2K1 Antibody Target Details MAP2K1 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-MAP2K1 antibody (Mitogen-Activated Protein Kinase Kinase 1) (C-Term) (ABIN189514) Western blot analysis on Jurkat cell lysate using anti-MEK1 (C-term) RabMAb (cat. #11...
Immunohistochemistry (IHC) image for anti-MAP2K1 antibody (Mitogen-Activated Protein Kinase Kinase 1) (C-Term) (ABIN189514) Immunohistochemical analysis of urinary bladder carcinoma using anti-MEK1 (C-term) Ra...