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anti-Human MAP2K1 antibody for Immunofluorescence

Recommended MAP2K1 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) Antibodies
  • ATMEK1
  • CFC3
  • F20B18.180
  • F20B18_180
  • MAP kinase/ ERK kinase 1
  • MAPKK1
  • MEK
  • mek-2
  • MEK1
  • Mek1
  • MEKK1
  • MITOGEN ACTIVATED PROTEIN KINASE KINASE
  • MITOGEN ACTIVATED PROTEIN KINASE KINASE 1
  • MKK1
  • NMAPKK
  • PRKMK1
  • Prkmk1
  • si:ch211-242m18.2
  • wu:fj56a12
  • wu:fj61b01
  • zgc:56557
Epitope
Center
43
37
32
32
32
31
29
27
25
22
18
15
15
13
13
13
11
9
7
7
6
6
6
5
5
4
4
4
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
587
311
269
56
38
20
15
13
10
6
4
2
2
Host
Rabbit
476
121
2
2
1
Clonality
Polyclonal
Conjugate
This MAP2K1 antibody is un-conjugated
14
14
14
11
9
8
8
8
6
6
6
6
6
6
6
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
434
200
156
112
87
66
23
22
10
10
9
6
6
2
2
2
2
2
Supplier
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Catalog No. ABIN2855379
$ 358.60
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.31815147 ABIN615073 IF IHC (p) WB Rabbit N-Term Log in to see Polyclonal
0.31815147 ABIN1870339 IF IHC WB Rabbit IgG pSer221 Log in to see Polyclonal
0.31815147 ABIN2706545 IC IF IHC WB Rabbit pSer292 Log in to see Polyclonal
0.31815147 ABIN2424909 IF IHC WB Rabbit IgG pSer221 Log in to see Polyclonal
0.0010831101 ABIN196888 IF IHC (p) WB Rabbit pSer221, pSer222 Log in to see Polyclonal 5
0.0010831101 ABIN498700 IF IHC (p) WB Rabbit pThr292 Log in to see Polyclonal
0.0010831101 ABIN189514 ICC FACS IF IHC (p) IP IHC WB Rabbit IgG C-Term Log in to see Y77
0.0010831101 ABIN362168 IF IHC WB Rabbit IgG pSer221 Log in to see Polyclonal 6
0.0010831101 ABIN1499379 IF IHC (p) WB Mouse IgG2b Log in to see 4H8
0.0010831101 ABIN1499377 IF IHC (p) WB Mouse IgG2b Log in to see 2A9
0.0010831101 ABIN1499386 IF IHC (p) WB Mouse IgG1 Log in to see 6C6
0.0010831101 ABIN1499378 IF IHC (p) WB Mouse IgG2b Log in to see 3H5
0.0010831101 ABIN1499389 IF IHC (p) WB Mouse IgG1 Log in to see 1A10
0.0010831101 ABIN1499388 IF IHC (p) WB Mouse IgG2b Log in to see 8A6
0.0010831101 ABIN1499392 IF IHC (p) WB Mouse IgG2b Log in to see 2A1
0.0010831101 ABIN1499396 IF WB Mouse IgG2a Log in to see 8H1
0.0010831101 ABIN1499382 IF IHC (p) WB Mouse IgG2a Log in to see 4E4
0.0010831101 ABIN1499383 IF IHC (p) WB Mouse IgG2b Log in to see 2F1
0.0010831101 ABIN1499380 IF IHC (p) WB Mouse IgG2b Log in to see 4F1
0.0010831101 ABIN1499394 IF IHC (p) WB Mouse IgG2a Log in to see 2F12

Top referenced anti-MAP2K1 antibody for Immunofluorescence

Similar anti-MAP2K1 Antibodies

Application / Reactivity Mouse (Murine) Human
BioImaging (BI) 2 Antibodies 2 Antibodies
ELISA 44 Antibodies 153 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies 6 Antibodies
Immunocytochemistry (ICC) 4 Antibodies 12 Antibodies
Immunofluorescence (IF) 52 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 66 Antibodies
Immunohistochemistry (IHC) 113 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 44 Antibodies
Immunoprecipitation (IP) 10 Antibodies
Intracellular Staining (ICS) 9 Antibodies
Western Blotting (WB) 221 Antibodies
Dot Blot (DB) 23 Antibodies
Flow Cytometry (FACS) 3 Antibodies
Immunochromatography (IC) 2 Antibodies

General

Antigen Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) Antibodies
Epitope Center
(43), (37), (32), (32), (32), (31), (29), (27), (25), (22), (18), (15), (15), (13), (13), (13), (11), (9), (7), (7), (6), (6), (6), (5), (5), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(587), (311), (269), (56), (38), (20), (15), (13), (10), (6), (4), (2), (2)
Host Rabbit
(476), (121), (2), (2), (1)
Clonality
Polyclonal
Conjugate This MAP2K1 antibody is un-conjugated
(14), (14), (14), (11), (9), (8), (8), (8), (6), (6), (6), (6), (6), (6), (6), (1), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
(434), (200), (156), (112), (87), (66), (23), (22), (10), (10), (9), (6), (6), (2), (2), (2), (2), (2)
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Product Details anti-MAP2K1 Antibody

Target Details MAP2K1 Application Details Handling Images
Cross-Reactivity Rat (Rattus), Dog (Canine), Rabbit, Rhesus Monkey, Chimpanzee, Cow (Bovine)
Cross-Reactivity (Details) Rat (100 %), Dog (100 %), Rabbit (100 %), Rhesus Monkey (100 %), Chimpanzee (100 %), Bovine (100 %)
Characteristics Rabbit Polyclonal antibody to MEK1 (mitogen-activated protein kinase kinase 1)
MEK1 antibody
Purification Purified by antigen-affinity chromatography.
Immunogen Recombinant protein encompassing a sequence within the center region of human MEK1. The exact sequence is proprietary.
Isotype IgG

Target Details MAP2K1

Product Details anti-MAP2K1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name MEK1 (MAP2K1 Antibody Abstract)
Background The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development.

Molecular Weight 43 kDa
Gene ID 5604
Research Area Signaling, Tyrosine Kinases
Pathways MAPK Signaling, RTK Signaling, TCR Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-MAP2K1 Antibody Target Details MAP2K1 Handling Images back to top
Application Notes Suggested dilution Reference ICC/IF 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* Western blot1:500-1:3000*
Comment

Positive Control: 293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji , mouse brain

Restrictions For Research Use only

Handling

Product Details anti-MAP2K1 Antibody Target Details MAP2K1 Application Details Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Images

Product Details anti-MAP2K1 Antibody Target Details MAP2K1 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-MAP2K1 antibody (Mitogen-Activated Protein Kinase Kinase 1) (Center) (ABIN2855379) WB Image Sample (30 ug of whole cell lysate) A: 293T 10% SDS PAGE antibody diluted at...
Immunofluorescence (IF) image for anti-MAP2K1 antibody (Mitogen-Activated Protein Kinase Kinase 1) (Center) (ABIN2855379) ICC/IF Image Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde...
Western Blotting (WB) image for anti-MAP2K1 antibody (Mitogen-Activated Protein Kinase Kinase 1) (Center) (ABIN2855379) WB Image Sample (50 ug of whole cell lysate) A: mouse brain 10% SDS PAGE antibody dil...