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anti-Human MAP2K1 antibody for Immunofluorescence (Paraffin-embedded Sections)

Recommended MAP2K1 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) Antibodies
  • ATMEK1
  • CFC3
  • F20B18.180
  • F20B18_180
  • MAP kinase/ ERK kinase 1
  • MAPKK1
  • MEK
  • mek-2
  • MEK1
  • Mek1
  • MEKK1
  • MITOGEN ACTIVATED PROTEIN KINASE KINASE
  • MITOGEN ACTIVATED PROTEIN KINASE KINASE 1
  • MKK1
  • NMAPKK
  • PRKMK1
  • Prkmk1
  • si:ch211-242m18.2
  • wu:fj56a12
  • wu:fj61b01
  • zgc:56557
Epitope
AA 2-35
43
37
32
32
32
31
29
27
25
22
18
15
15
13
13
12
11
9
7
7
6
6
6
6
5
4
4
4
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
586
311
267
56
38
20
14
13
10
6
4
2
2
Host
Rabbit
476
120
2
2
1
Clonality
Polyclonal
Conjugate
This MAP2K1 antibody is un-conjugated
14
14
14
11
9
8
8
8
6
6
6
6
6
6
6
1
1
Application
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
433
200
156
113
86
65
23
21
11
10
9
6
6
2
2
2
2
2
Supplier
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Supplier Product No.
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Available images

Catalog No. ABIN686482
$ 269.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.000881264 ABIN733703 IF (p) IHC (p) WB Rabbit IgG AA 276-309, pSer298 Log in to see Polyclonal
0.000881264 ABIN801708 IF (p) IHC (p) WB Rabbit IgG pThr386 Log in to see Polyclonal
0.000881264 ABIN906098 IF (p) Alexa Fluor 555 Rabbit IgG AA 2-35 Log in to see Polyclonal
0.000881264 ABIN906099 IF (p) Alexa Fluor 647 Rabbit IgG AA 2-35 Log in to see Polyclonal
0.000881264 ABIN906097 IF (p) Alexa Fluor 350 Rabbit IgG AA 2-35 Log in to see Polyclonal
0.000881264 ABIN906100 IF (p) Alexa Fluor 488 Rabbit IgG AA 2-35 Log in to see Polyclonal
0.000881264 ABIN686488 IF (p) Cy7 Rabbit IgG AA 2-35 Log in to see Polyclonal
0.000881264 ABIN686485 IF (p) Cy3 Rabbit IgG AA 2-35 Log in to see Polyclonal
0.000881264 ABIN686487 IF (p) Cy5.5 Rabbit IgG AA 2-35 Log in to see Polyclonal
0.000881264 ABIN686489 IF (p) FITC Rabbit IgG AA 2-35 Log in to see Polyclonal
0.000881264 ABIN686486 IF (p) Cy5 Rabbit IgG AA 2-35 Log in to see Polyclonal
0.000881264 ABIN2804111 IF (p) Alexa Fluor 594 Rabbit IgG AA 2-35 Log in to see Polyclonal
0.000881264 ABIN877922 IF (p) Alexa Fluor 647 Rabbit IgG pThr286 Log in to see Polyclonal
0.000881264 ABIN877923 IF (p) Alexa Fluor 350 Rabbit IgG pThr286 Log in to see Polyclonal
0.000881264 ABIN877924 IF (p) Alexa Fluor 555 Rabbit IgG pThr286 Log in to see Polyclonal
0.000881264 ABIN877926 IF (p) Alexa Fluor 488 Rabbit IgG pThr286 Log in to see Polyclonal
0.000881264 ABIN877942 IF (p) Alexa Fluor 350 Rabbit IgG pThr292 Log in to see Polyclonal
0.000881264 ABIN877944 IF (p) Alexa Fluor 555 Rabbit IgG pThr292 Log in to see Polyclonal
0.000881264 ABIN877945 IF (p) Alexa Fluor 647 Rabbit IgG pThr292 Log in to see Polyclonal
0.000881264 ABIN877943 IF (p) Alexa Fluor 488 Rabbit IgG pThr292 Log in to see Polyclonal

Similar anti-MAP2K1 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
BioImaging (BI) 2 Antibodies 2 Antibodies
ELISA 45 Antibodies 44 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies 3 Antibodies
Immunocytochemistry (ICC) 5 Antibodies 4 Antibodies
Immunofluorescence (IF) 48 Antibodies 52 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 55 Antibodies 66 Antibodies
Immunohistochemistry (IHC) 105 Antibodies 113 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 38 Antibodies 44 Antibodies
Immunoprecipitation (IP) 7 Antibodies
Intracellular Staining (ICS) 3 Antibodies
Western Blotting (WB) 195 Antibodies

General

Antigen Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) Antibodies
Epitope AA 2-35
(43), (37), (32), (32), (32), (31), (29), (27), (25), (22), (18), (15), (15), (13), (13), (12), (11), (9), (7), (7), (6), (6), (6), (6), (5), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(586), (311), (267), (56), (38), (20), (14), (13), (10), (6), (4), (2), (2)
Host Rabbit
(476), (120), (2), (2), (1)
Clonality
Polyclonal
Conjugate This MAP2K1 antibody is un-conjugated
(14), (14), (14), (11), (9), (8), (8), (8), (6), (6), (6), (6), (6), (6), (6), (1), (1)
Application Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(433), (200), (156), (113), (86), (65), (23), (21), (11), (10), (9), (6), (6), (2), (2), (2), (2), (2)
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Product Details anti-MAP2K1 Antibody

Target Details MAP2K1 Application Details Handling Images
Purification Purified by Protein A.
Immunogen KLH conjugated synthetic peptide derived from human MEK1
Isotype IgG

Target Details MAP2K1

Product Details anti-MAP2K1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name Mek1 (MAP2K1 Antibody Abstract)
Background

Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Binding of extracellular ligands such as growth factors, cytokines and hormones to their cell-surface receptors activates RAS and this initiates RAF1 activation. RAF1 then further activates the dual-specificity protein kinases MAP2K1/MEK1 and MAP2K2/MEK2. Both MAP2K1/MEK1 and MAP2K2/MEK2 function specifically in the MAPK/ERK cascade, and catalyze the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in the extracellular signal-regulated kinases MAPK3/ERK1 and MAPK1/ERK2, leading to their activation and further transduction of the signal within the MAPK/ERK cascade. Depending on the cellular context, this pathway mediates diverse biological functions such as cell growth, adhesion, survival and differentiation, predominantly through the regulation of transcription, metabolism and cytoskeletal rearrangements. One target of the MAPK/ERK cascade is peroxisome proliferator-activated receptor gamma (PPARG), a nuclear receptor that promotes differentiation and apoptosis. MAP2K1/MEK1 has been shown to export PPARG from the nucleus. The MAPK/ERK cascade is also involved in the regulation of endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC), as well as in the fragmentation of the Golgi apparatus during mitosis.


Synonyms: CFC3, MEK1, MKK1, MAPKK1, PRKMK1, Dual specificity mitogen-activated protein kinase kinase 1, MAP kinase kinase 1, MAPKK 1, ERK activator kinase 1, MAPK/ERK kinase 1, MEK 1, MAP2K1
Gene ID 5604
UniProt Q02750
Research Area Signaling, Tyrosine Kinases
Pathways MAPK Signaling, RTK Signaling, TCR Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-MAP2K1 Antibody Target Details MAP2K1 Handling Images back to top
Application Notes WB(1:100-1000), IHC-P(1:100-500), IF(IHC-P)(1:50-200)
Restrictions For Research Use only

Handling

Product Details anti-MAP2K1 Antibody Target Details MAP2K1 Application Details Images back to top
Format Liquid
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 100 μg/mL BSA, 50 % glycerol and 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20°C
Expiry Date 12 months

Images

Product Details anti-MAP2K1 Antibody Target Details MAP2K1 Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-MAP2K1 antibody (Mitogen-Activated Protein Kinase Kinase 1) (AA 2-35) (ABIN686482) Images provided the Independent Validation Program (badge number 029646)Formalin-fixe...
'Independent Validation' Badge
Lot Number 90915
Method validated Immunohistochemistry
Positive Control Human placenta
Negative Control Human breast adipocytes
Notes Signal detected in positive control sample and not in negative control sample.
Primary Antibody
  • Antigen: Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1)
  • Catalog number: ABIN686482
  • Supplier: Log in to see
  • Supplier catalog number: Log in to see
  • Lot number: 90915
Isotype Control Antibody
Secondary Antibody
  • Antibody: Biotinylated goat anti-rabbit/anti-mouse (Kit)
  • Supplier: Log in to see
  • Catalog number: Log in to see
  • Lot number: D07640BA
Controls
  • Positive control: Human placenta tissue stained with antibody
  • Negative control: Human breast adipocytes tissue stained with antibody
  • Isotype control: Human placenta tissue stained with isotype control
  • Secondary only control: Human placenta tissue stained with secondary antibody only
Protocol
  • Immunohistochemistry was performed on a Ventana NEXes automated platform; instrument manufacturer specific reagents are italicized.
  • 1. Slides were preheated in convection oven at 60°C for 30 min
  • 2. Deparaffinization procedure: - 3 changes of Xylene, 5 min each - 3 changes of 100% Ethanol, 3 min each - 3 changes of 95% Ethanol, 3 min each - Rinsed in distilled water, 3 changes
  • 3. Heat retrieval procedure - Slides retrieved in 10.0 mM Citrate, pH6.0 in a 1000W microwave oven (~100°C) for 15 min. - Slides were allowed to cool (in citrate) for 30 min. - Slides were washed x 3 in Distilled water
  • 4. NEXes instrument procedure, iView DAB paraffin protocol (*abridged*): - Slide chamber warmed to 37°C
  • 5. Slides rinsed with *reaction buffer* x3
  • 6. *iView Inhibitor (H2O2)* applied and incubated for 4 min
  • 7. Slides rinsed with *reaction buffer*
  • 8. Antibody Application - Primary antibody diluted 1:250 in PBS (100 microliter applied/slide) - Ventana Isotype control applied neat - Slides Incubated overnight at room temperature (~12 hours ~25°C)
  • 9. Slides rinsed with *reaction buffer* x3
  • 10. *iView Biotinylated IgG* applied and incubated for 8 min
  • 11. Slides rinsed with *reaction buffer*
  • 14. *iView Streptavidin-Horseradish Peroxidase* applied and incubated for 8 min
  • 15. Slides rinsed with *reaction buffer*
  • 16. *iView DAB/H2O2* applied and incubated for 8 min
  • 17. Slides rinsed with *reaction buffer*
  • 18. *iView Copper* applied and incubated for 4 min
  • 19. Slides rinsed with *reaction buffer*
  • 20. Slides washed in Dawn Detergent/tap water
  • 21. Counterstain Procedure - Hematoxylin (Leica 560 MX) 30 sec - Slides washed in tap water, 1 min - Decolorized (10% Acetic Acid in 70% ethanol), 1 min - Slides washed in tap water, 1 min - Bluing (Austin Clear Ammonia), 1 min - Slides washed in tap water, 1 min
  • 22. Dehydration/coverslipping procedure: - 3 changes of 95% Ethanol, 3 min each - 3 changes of 100% Ethanol, 3 min each - 3 changes of Xylene, 5 min each - Mounted with Permount
  • 23. Imaging: Leica SCN 400F Whole Slide Scanner with Digital Image Hub and Leica Slidepath software
Experimental Notes
  • Deviations from protocol/procedure supplied by manufacturer:
  • Step 1: Heated tissue 60°C for 30 minutes; manufacturer heats for 45 minutes.
  • Step 2: No ethanol wash was performed during deparaffinization; manufacturer includes 1 wash of 80% ethanol for 3 minutes.
  • Step 3.1: Slides were heated for 15 minutes; manufacturer provides a range of 15-20 minutes.
  • Step 3.2: Slides were cooled for 30 minutes; manufacturer cools for 20 minutes.
  • Step 4: Italicized reagents and incubation time are fixed instrument parameters.
  • Step 5: Secondary species-specific serum block not used; manufacturer blocks with 5% normal goat serum for 2 hours.
  • Step 8.1: Antibody diluted in PBS at 1:250; manufacture did not recommend diluent or dilution.
  • Step 8.2.1: Primary antibody incubated at room temperature overnight; manufacturer incubates overnight 4°C with agitation.
Validation Images
Immunohistochemistry validation image for anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (AA 2-35) antibody (ABIN686482) Figure 1: Human placenta tissue stained with anti-MAP2K1 (brown) and counterstained w...
Immunohistochemistry validation image for anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (AA 2-35) antibody (ABIN686482) Figure 2: Human breast adipose tissue stained with anti-MAP2K1 (brown) and countersta...
Immunohistochemistry validation image for anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (AA 2-35) antibody (ABIN686482) Figure 3: Human placenta tissue stained with isotype control antibody (brown) and cou...
Immunohistochemistry validation image for anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (AA 2-35) antibody (ABIN686482) Figure 4: Human placenta tissue stained with secondary antibody only (brown) and coun...