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anti-Human MAP2K1 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended MAP2K1 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) Antibodies
  • ATMEK1
  • CFC3
  • F20B18.180
  • F20B18_180
  • MAP kinase/ ERK kinase 1
  • MAPKK1
  • MEK
  • mek-2
  • MEK1
  • Mek1
  • MEKK1
  • MITOGEN ACTIVATED PROTEIN KINASE KINASE
  • MITOGEN ACTIVATED PROTEIN KINASE KINASE 1
  • MKK1
  • NMAPKK
  • PRKMK1
  • Prkmk1
  • si:ch211-242m18.2
  • wu:fj56a12
  • wu:fj61b01
  • zgc:56557
Epitope
pThr292
43
38
35
32
32
31
29
27
25
22
21
15
15
13
13
13
11
9
7
7
6
6
6
6
5
4
4
4
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
607
323
280
60
38
24
15
14
13
6
4
4
4
2
2
Host
Rabbit
494
124
2
2
1
Clonality
Polyclonal
Conjugate
This MAP2K1 antibody is un-conjugated
14
14
14
11
9
8
8
8
6
6
6
6
6
6
6
1
1
Application
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
455
208
159
115
94
66
23
22
14
10
9
6
6
2
2
2
2
2
1
Supplier
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Catalog No. ABIN498700
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.20781031 ABIN498721 IHC (p) WB Rabbit pSer222, pSer226 Log in to see Polyclonal
0.20781031 ABIN498720 IHC (p) WB Rabbit pSer218, pSer222 Log in to see Polyclonal
0.20781031 ABIN498855 IHC (p) Rabbit pSer222 Log in to see Polyclonal
0.20781031 ABIN498854 IHC (p) Rabbit pSer218 Log in to see Polyclonal
0.20781031 ABIN498600 IHC (p) WB Rabbit Log in to see Polyclonal
0.20781031 ABIN498942 IHC (p) Rabbit Log in to see Polyclonal
0.20781031 ABIN498654 IHC (p) WB Rabbit Log in to see Polyclonal
0.20781031 ABIN498943 IHC (p) Rabbit Log in to see Polyclonal
0.20781031 ABIN615073 IF IHC (p) WB Rabbit N-Term Log in to see Polyclonal
0.20781031 ABIN4333485 ICC IF IHC IHC (p) WB Mouse IgG1 Log in to see 1F5 1
0.20781031 ABIN461964 IF IHC (p) WB Rabbit N-Term Log in to see Polyclonal
0.20781031 ABIN4333486 ICC IF IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal 1
0.20781031 ABIN4333482 ICC IF IHC IHC (p) WB Rabbit pThr292 Log in to see Polyclonal
0.20781031 ABIN4333480 IHC IHC (p) WB Rabbit Log in to see Polyclonal
0.20781031 ABIN4333481 IHC IHC (p) WB Rabbit Log in to see Polyclonal
0.00089362357 ABIN686482 IF (p) IHC (p) WB Rabbit IgG AA 2-35 Log in to see Polyclonal
0.00089362357 ABIN358157 EIA IHC (p) WB Rabbit Ig pSer217, pSer221 Log in to see Polyclonal 5
0.00089362357 ABIN196944 IHC (p) WB Rabbit pSer218, pSer221 Log in to see Polyclonal 2
0.00089362357 ABIN197562 IHC (p) WB Rabbit Thr291 Log in to see Polyclonal 3
0.00089362357 ABIN196888 IF IHC (p) WB Rabbit pSer221, pSer222 Log in to see Polyclonal 5

Top referenced anti-MAP2K1 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Similar anti-MAP2K1 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
BioImaging (BI) 2 Antibodies 2 Antibodies
ELISA 46 Antibodies 45 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies 3 Antibodies
Immunocytochemistry (ICC) 7 Antibodies 5 Antibodies
Immunofluorescence (IF) 50 Antibodies 53 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 55 Antibodies 66 Antibodies
Immunohistochemistry (IHC) 110 Antibodies 117 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 43 Antibodies
Immunoprecipitation (IP) 8 Antibodies
Intracellular Staining (ICS) 3 Antibodies
Simple Western (SimWes) 1 Antibodies
Western Blotting (WB) 206 Antibodies

General

Antigen Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) Antibodies
Epitope pThr292
(43), (38), (35), (32), (32), (31), (29), (27), (25), (22), (21), (15), (15), (13), (13), (13), (11), (9), (7), (7), (6), (6), (6), (6), (5), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(607), (323), (280), (60), (38), (24), (15), (14), (13), (6), (4), (4), (4), (2), (2)
Host Rabbit
(494), (124), (2), (2), (1)
Clonality
Polyclonal
Conjugate This MAP2K1 antibody is un-conjugated
(14), (14), (14), (11), (9), (8), (8), (8), (6), (6), (6), (6), (6), (6), (6), (1), (1)
Application Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(455), (208), (159), (115), (94), (66), (23), (22), (14), (10), (9), (6), (6), (2), (2), (2), (2), (2), (1)
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Product Details anti-MAP2K1 Antibody

Target Details MAP2K1 Application Details Handling Images
Specificity This antibody detects endogenous levels of Cdc25A protein only when phosphorylated at Thr292.
Cross-Reactivity (Details) Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
Purification Affinity chromatography
Purity > 95 % by SDS-PAGE

Target Details MAP2K1

Product Details anti-MAP2K1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name MAP2K1 (MAP2K1 Antibody Abstract)
Background MEK1 (Mitogen activated protein kinase kinase 1) catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. MEK1 activates ERK1 and ERK2 MAP kinases. Mitogen activated protein kinase kinase 2 (MEK2 or MAPKK2) is a member of a family of tyrosine/threonine protein kinases that activate the ERK1 and 2 and MAPK enzymes by phosphorylating both residues within the threonine/glutamate/tyrosine (TEY) motif in the activation loop. MEK1 and 2 are also activated by dual phosphorylation, which occurs on serine 218 and 222, in the activation loop of the MEK. Threonine 292 of MEK1 is phosphorylated by ERK 2, which serves as a negative feedback loop by suppressing activation of MEK1.Synonyms: Dual specificity mitogen-activated protein kinase kinase 1, ERK activator kinase 1, MAP kinase kinase 1, MAPK/ERK kinase 1, MAPKK 1, MEK1, PRKMK1
Molecular Weight approx. 43 kDa
Gene ID 5604
NCBI Accession NP_002746
UniProt Q02750
Research Area Kinases/Phosphatases, Phospho-specific antibodies
Pathways MAPK Signaling, RTK Signaling, TCR Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-MAP2K1 Antibody Target Details MAP2K1 Handling Images back to top
Application Notes Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200. Immunofluorescence: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-MAP2K1 Antibody Target Details MAP2K1 Application Details Images back to top
Concentration 1,0 mg/mL
Buffer Phosphate buffered saline (PBS), pH 7.2., 15 mM sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Images

Product Details anti-MAP2K1 Antibody Target Details MAP2K1 Application Details Handling back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-MAP2K1 antibody (Mitogen-Activated Protein Kinase Kinase 1) (pThr292) (ABIN498700) Immunohistochemistry (IHC) analyzes of p-MEK1 antibody in paraffin-embedded hu...
Western Blotting (WB) image for anti-MAP2K1 antibody (Mitogen-Activated Protein Kinase Kinase 1) (pThr292) (ABIN498700) Western blot (WB) analysis of p-MEK1 antibody in extracts from K562 cells.