You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Mouse (Murine) MAP2K1 antibody for Intracellular Staining

Recommended MAP2K1 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) Antibodies
  • ATMEK1
  • CFC3
  • F20B18.180
  • F20B18_180
  • MAP kinase/ ERK kinase 1
  • MAPKK1
  • MEK
  • mek-2
  • MEK1
  • Mek1
  • MEKK1
  • MITOGEN ACTIVATED PROTEIN KINASE KINASE
  • MITOGEN ACTIVATED PROTEIN KINASE KINASE 1
  • MKK1
  • NMAPKK
  • PRKMK1
  • Prkmk1
  • si:ch211-242m18.2
  • wu:fj56a12
  • wu:fj61b01
  • zgc:56557
Epitope
pSer298
43
37
34
32
32
31
30
27
25
22
20
15
15
13
13
13
11
9
7
7
6
6
6
6
5
4
4
4
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
605
322
280
60
38
24
15
14
13
6
4
4
4
2
2
Host
Mouse
493
122
2
2
1
Clonality
Monoclonal
Conjugate
This MAP2K1 antibody is conjugated to PE
14
14
14
10
9
8
8
8
6
6
6
6
6
6
6
1
1
Application
Intracellular Staining (ICS)
453
208
157
116
95
66
23
22
14
10
8
6
6
2
2
2
2
2
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Catalog No. ABIN1177090
Contact our Customer Service for availability and price in your country.
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00087119907 ABIN1177092 ICS Alexa Fluor 488 Mouse IgG1, kappa pSer298 Log in to see J114-64 3
0.00087119907 ABIN1177091 ICS Alexa Fluor 647 Mouse IgG1, kappa pSer298 Log in to see J114-64 3
0.00087119907 ABIN1176908 ICS PE Mouse IgG2a Log in to see 25-MEK1 3
0.00087119907 ABIN1176909 ICS Alexa Fluor 647 Mouse IgG2a Log in to see 25-MEK1 3
0.00087119907 ABIN1177093 ICS WB Mouse IgG1, kappa pSer218 Log in to see O24 5
0.00087119907 ABIN1177094 ICS PE Mouse IgG1, kappa pSer218 Log in to see O24 5
0.00087119907 ABIN1177095 ICS Alexa Fluor 647 Mouse IgG1, kappa pSer218 Log in to see O24 5
0.00087119907 ABIN1177096 ICS Alexa Fluor 488 Mouse IgG1, kappa pSer218 Log in to see O24 5

Top referenced anti-MAP2K1 antibody for Intracellular Staining

Similar anti-MAP2K1 Antibodies

Application / Reactivity Human Mouse (Murine)
Western Blotting (WB) 441 Antibodies 232 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Luminex Assay (LMNX) 10 Antibodies
Intracellular Staining (ICS) 9 Antibodies 9 Antibodies
Immunoprecipitation (IP) 14 Antibodies 10 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 96 Antibodies 49 Antibodies
Immunohistochemistry (IHC) 209 Antibodies 118 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 3 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 66 Antibodies 66 Antibodies
Immunofluorescence (IF) 117 Antibodies 53 Antibodies
Immunodiffusion (ID) 6 Antibodies

General

Antigen Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) Antibodies
Epitope pSer298
(43), (37), (34), (32), (32), (31), (30), (27), (25), (22), (20), (15), (15), (13), (13), (13), (11), (9), (7), (7), (6), (6), (6), (6), (5), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(605), (322), (280), (60), (38), (24), (15), (14), (13), (6), (4), (4), (4), (2), (2)
Host Mouse
(493), (122), (2), (2), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This MAP2K1 antibody is conjugated to PE
(14), (14), (14), (10), (9), (8), (8), (8), (6), (6), (6), (6), (6), (6), (6), (1), (1)
Application Intracellular Staining (ICS)
(453), (208), (157), (116), (95), (66), (23), (22), (14), (10), (8), (6), (6), (2), (2), (2), (2), (2)
Pubmed 3 references available
Supplier Log in to see

Product Details anti-MAP2K1 Antibody

Target Details MAP2K1 Application Details Handling References for anti-MAP2K1 Antibody (ABIN1177090) Images
Brand BD Phosflow™
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Phosphorylated Human MEK1 Peptide
Clone J114-64
Isotype IgG1, kappa

Target Details MAP2K1

Product Details anti-MAP2K1 Antibody Application Details Handling References for anti-MAP2K1 Antibody (ABIN1177090) Images back to top
Antigen
Alternative Name MEK1 (MAP2K1 Antibody Abstract)
Background MEK (Map/Erk Kinase) 1 and 2 are serine/threonine kinases, also known as MAP kinase kinases (MAP2K1 and 2, MAPKK1 and 2, or MKK1 and 2). They activate the MAP (Mitogen-Activated Protein) kinases, also known as ERKs (Extracellular signal Regulated Kinases), which are critical kinases in multiple signal transduction pathways that regulate cell growth and differentiation. Activation of MEK 1 and 2 is dependent upon phosphorylation of serines 218 and/or 222 by activated MAP kinase kinase kinases (MAP3Ks), such as the Raf isoforms. Hormones, growth and differentiating factors, or tumor promoters induce Raf activation via activation of Ras proteins. Alternatively, cellular adhesion can lead to phosphorylation of MEK1 at serine 298 (S298), mediated by p21-activated kinase (PAK). The S298-phosphorylated MEK1 has an enhanced capacity to interact with Raf, resulting in MEK1 activation. The J114-64 monoclonal antibody recognizes the phosphorylated S298 of MEK1.
Research Area Tyrosine Kinases
Pathways MAPK Signaling, RTK Signaling, TCR Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-MAP2K1 Antibody Target Details MAP2K1 Handling References for anti-MAP2K1 Antibody (ABIN1177090) Images back to top
Application Notes Either BD Cytofix™ buffer or BD™ Phosflow Fix Buffer I may be used for cell fixation.
Sample Volume 20 μL
Restrictions For Research Use only

Handling

Product Details anti-MAP2K1 Antibody Target Details MAP2K1 Application Details References for anti-MAP2K1 Antibody (ABIN1177090) Images back to top
Format Liquid
Buffer Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References for anti-MAP2K1 Antibody (ABIN1177090)

Product Details anti-MAP2K1 Antibody Target Details MAP2K1 Application Details Handling Images back to top
Product cited in:

Slack-Davis, Eblen, Zecevic, Boerner, Tarcsafalvi, Diaz, Marshall, Weber, Parsons, Catling: "PAK1 phosphorylation of MEK1 regulates fibronectin-stimulated MAPK activation." in: The Journal of cell biology, Vol. 162, Issue 2, pp. 281-91, 2003

Eblen, Slack, Weber, Catling: "Rac-PAK signaling stimulates extracellular signal-regulated kinase (ERK) activation by regulating formation of MEK1-ERK complexes." in: Molecular and cellular biology, Vol. 22, Issue 17, pp. 6023-33, 2002

Kolch: "Meaningful relationships: the regulation of the Ras/Raf/MEK/ERK pathway by protein interactions." in: The Biochemical journal, Vol. 351 Pt 2, Issue 2, pp. 289-305, 2001