You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Non Human Primates MAP2K1 antibody for Western Blotting

Recommended MAP2K1 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) Antibodies
  • Mek1
  • CFC3
  • MAPKK1
  • MEK1
  • MKK1
  • PRKMK1
  • MEKK1
  • Prkmk1
  • mek-2
  • si:ch211-242m18.2
  • wu:fj56a12
  • wu:fj61b01
  • zgc:56557
  • ATMEK1
  • F20B18.180
  • F20B18_180
  • MAP kinase/ ERK kinase 1
  • MITOGEN ACTIVATED PROTEIN KINASE KINASE
  • MITOGEN ACTIVATED PROTEIN KINASE KINASE 1
  • NMAPKK
  • MEK
  • mitogen activated protein kinase kinase 1
  • mitogen-activated protein kinase kinase 1
  • Map2k1
  • MAP2K1
  • map2k1
  • MEK1
Epitope
pThr386
36
34
33
32
32
30
26
25
24
23
19
15
15
15
15
14
12
11
9
8
6
5
5
5
4
4
4
4
3
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Chicken, Human, Mouse (Murine), Non Human Primates, Rat (Rattus), Xenopus laevis
605
417
366
107
80
44
30
25
21
21
10
8
5
5
4
4
2
2
1
1
1
Host
Rabbit
487
124
2
2
1
Clonality
Polyclonal
Conjugate
This MAP2K1 antibody is un-conjugated
14
14
14
11
9
8
8
8
6
6
6
6
6
6
6
6
6
2
2
Application
Western Blotting (WB)
468
195
150
123
120
78
25
22
16
10
9
7
6
6
2
2
2
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN372666
$ 412.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN372665 WB Rabbit IgG pThr292 Log in to see Polyclonal 1

Top referenced anti-MAP2K1 antibody for Western Blotting

Similar anti-MAP2K1 Antibodies

Application / Reactivity Human Cow (Bovine) Dog (Canine) Mouse (Murine) Rat (Rattus) Xenopus laevis Chicken Non Human Primates
Immunofluorescence (fixed cells) (IF/ICC) 5 Antibodies 1 Antibodies 1 Antibodies 3 Antibodies 2 Antibodies
Simple Western (SimWes) 1 Antibodies 1 Antibodies
Western Blotting (WB) 458 Antibodies 310 Antibodies 278 Antibodies 24 Antibodies 103 Antibodies 39 Antibodies 18 Antibodies 2 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies 1 Antibodies

General

Antigen Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) Antibodies
Epitope pThr386
(36), (34), (33), (32), (32), (30), (26), (25), (24), (23), (19), (15), (15), (15), (15), (14), (12), (11), (9), (8), (6), (5), (5), (5), (4), (4), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Chicken, Human, Mouse (Murine), Non Human Primates, Rat (Rattus), Xenopus laevis
(605), (417), (366), (107), (80), (44), (30), (25), (21), (21), (10), (8), (5), (5), (4), (4), (2), (2), (1), (1), (1)
Host Rabbit
(487), (124), (2), (2), (1)
Clonality
Polyclonal
Conjugate This MAP2K1 antibody is un-conjugated
(14), (14), (14), (11), (9), (8), (8), (8), (6), (6), (6), (6), (6), (6), (6), (6), (6), (2), (2)
Application Western Blotting (WB)
(468), (195), (150), (123), (120), (78), (25), (22), (16), (10), (9), (7), (6), (6), (2), (2), (2), (1), (1), (1)
Pubmed 1 reference available
Supplier Log in to see

Product Details anti-MAP2K1 Antibody

Target Details MAP2K1 Application Details Handling References for anti-MAP2K1 Antibody (ABIN372666) Images
Specificity This antibody is specific for the ~45k MEK 1 protein phosphorylated at Thr386. The immunolabeling is completely eliminated by treatment with lambda-phosphatase.
Cross-Reactivity (Details) Species reactivity (expected):Mouse, Human, Chicken, Bovine, Canine, non-Human Primates and Xenopus.
Species reactivity (tested):Rat.
Purification Sequential Chromatography on phospho- and dephosphopeptide affinity columns.
Immunogen Phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr386 of human MEK 1.
Isotype IgG

Target Details MAP2K1

Product Details anti-MAP2K1 Antibody Application Details Handling References for anti-MAP2K1 Antibody (ABIN372666) Images back to top
Antigen
Alternative Name MAP2K1 (MAP2K1 Antibody Abstract)
Background MEK1 (MAP Kinase Kinase, also known as MKK) is an integral component of the MAP kinase cascade that regulates cell growth and differentiation (Ahn, 1993, Chong et al., 2003). This pathway also plays a key role in synaptic plasticity in the brain (Adams and Sweatt, 2002). Activated MEK 1 acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on MAP kinase (Kyriakis et al., 1991, Seger et al., 1991, Crews et al., 1992). Conversely, there also appears to be a feedback phosphorylation of MEK 1 by MAP kinase. The sites on MEK 1 that are phosphorylated by MAP kinase are Thr292 and Thr386 (Mansour et al., 1994).Synonyms: Dual specificity mitogen-activated protein kinase kinase 1, ERK activator kinase 1, MAP kinase kinase 1, MAPK/ERK kinase 1, MAPKK 1, MEK1, PRKMK1
Gene ID 5604
NCBI Accession NP_002746
UniProt Q02750
Research Area Kinases/Phosphatases, Phospho-specific antibodies
Pathways MAPK Signaling, RTK Signaling, TCR Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Autophagy, Signaling of Hepatocyte Growth Factor Receptor

Application Details

Product Details anti-MAP2K1 Antibody Target Details MAP2K1 Handling References for anti-MAP2K1 Antibody (ABIN372666) Images back to top
Application Notes Western blot: 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-MAP2K1 Antibody Target Details MAP2K1 Application Details References for anti-MAP2K1 Antibody (ABIN372666) Images back to top
Format Liquid
Buffer 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody undiluted (in aliquots) at-20 °C.

References for anti-MAP2K1 Antibody (ABIN372666)

Product Details anti-MAP2K1 Antibody Target Details MAP2K1 Application Details Handling Images back to top
Background publications

Adams, Sweatt: "Molecular psychology: roles for the ERK MAP kinase cascade in memory." in: Annual review of pharmacology and toxicology, Vol. 42, pp. 135-63, 2002

Images

Product Details anti-MAP2K1 Antibody Target Details MAP2K1 Application Details Handling References for anti-MAP2K1 Antibody (ABIN372666) back to top
Supplier Images
 image for anti-MAP2K1 antibody (Mitogen-Activated Protein Kinase Kinase 1) (pThr386) (ABIN372666) anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (pThr386) antibody