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|Application / Reactivity||Human||Cow (Bovine)||Dog (Canine)||Mouse (Murine)||Rat (Rattus)||Xenopus laevis||Chicken||Non Human Primates|
|Immunofluorescence (fixed cells) (IF/ICC)||5 Antibodies||1 Antibodies||1 Antibodies||3 Antibodies||2 Antibodies|
|Simple Western (SimWes)||1 Antibodies||1 Antibodies|
|Western Blotting (WB)||458 Antibodies||310 Antibodies||278 Antibodies||24 Antibodies||103 Antibodies||39 Antibodies||18 Antibodies||2 Antibodies|
|Proximity Ligation Assay (PLA)||1 Antibodies||1 Antibodies|
|Antigen||Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) Antibodies|
|Reactivity||Cow (Bovine), Dog (Canine), Chicken, Human, Mouse (Murine), Non Human Primates, Rat (Rattus), Xenopus laevis Alternatives|
|Conjugate||This MAP2K1 antibody is un-conjugated Alternatives|
Western Blotting (WB)
|1 reference available|
|Supplier||Log in to see|
Product Details anti-MAP2K1 AntibodyTarget Details MAP2K1 Application Details Handling References for anti-MAP2K1 Antibody (ABIN372666) Images
|Specificity||This antibody is specific for the ~45k MEK 1 protein phosphorylated at Thr386. The immunolabeling is completely eliminated by treatment with lambda-phosphatase.|
Species reactivity (expected):Mouse, Human, Chicken, Bovine, Canine, non-Human Primates and Xenopus.
Species reactivity (tested):Rat.
|Purification||Sequential Chromatography on phospho- and dephosphopeptide affinity columns.|
|Immunogen||Phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr386 of human MEK 1.|
Target Details MAP2K1Product Details anti-MAP2K1 Antibody Application Details Handling References for anti-MAP2K1 Antibody (ABIN372666) Images back to top
|Alternative Name||MAP2K1 (MAP2K1 Antibody Abstract)|
|Background||MEK1 (MAP Kinase Kinase, also known as MKK) is an integral component of the MAP kinase cascade that regulates cell growth and differentiation (Ahn, 1993, Chong et al., 2003). This pathway also plays a key role in synaptic plasticity in the brain (Adams and Sweatt, 2002). Activated MEK 1 acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on MAP kinase (Kyriakis et al., 1991, Seger et al., 1991, Crews et al., 1992). Conversely, there also appears to be a feedback phosphorylation of MEK 1 by MAP kinase. The sites on MEK 1 that are phosphorylated by MAP kinase are Thr292 and Thr386 (Mansour et al., 1994).Synonyms: Dual specificity mitogen-activated protein kinase kinase 1, ERK activator kinase 1, MAP kinase kinase 1, MAPK/ERK kinase 1, MAPKK 1, MEK1, PRKMK1|
|Research Area||Kinases/Phosphatases, Phospho-specific antibodies|
|Pathways||MAPK Signaling, RTK Signaling, TCR Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Autophagy, Signaling of Hepatocyte Growth Factor Receptor|
Application DetailsProduct Details anti-MAP2K1 Antibody Target Details MAP2K1 Handling References for anti-MAP2K1 Antibody (ABIN372666) Images back to top
Western blot: 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-MAP2K1 Antibody Target Details MAP2K1 Application Details References for anti-MAP2K1 Antibody (ABIN372666) Images back to top
|Buffer||10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage Comment||Store the antibody undiluted (in aliquots) at-20 °C.|
References for anti-MAP2K1 Antibody (ABIN372666)Product Details anti-MAP2K1 Antibody Target Details MAP2K1 Application Details Handling Images back to top
Adams, Sweatt: "Molecular psychology: roles for the ERK MAP kinase cascade in memory." in: Annual review of pharmacology and toxicology, Vol. 42, pp. 135-63, 2002
ImagesProduct Details anti-MAP2K1 Antibody Target Details MAP2K1 Application Details Handling References for anti-MAP2K1 Antibody (ABIN372666) back to top