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anti-Rat (Rattus) MAP2K1 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended MAP2K1 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) Antibodies
  • ATMEK1
  • CFC3
  • F20B18.180
  • F20B18_180
  • MAP kinase/ ERK kinase 1
  • MAPKK1
  • MEK
  • mek-2
  • MEK1
  • Mek1
  • MEKK1
  • MITOGEN ACTIVATED PROTEIN KINASE KINASE
  • MITOGEN ACTIVATED PROTEIN KINASE KINASE 1
  • MKK1
  • NMAPKK
  • PRKMK1
  • Prkmk1
  • si:ch211-242m18.2
  • wu:fj56a12
  • wu:fj61b01
  • zgc:56557
Epitope
pSer222, pSer226
43
38
34
32
32
32
30
27
25
22
21
15
15
13
13
13
11
9
7
7
6
6
6
6
5
4
4
4
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
606
323
280
60
38
24
15
14
13
6
4
4
4
2
2
Host
Rabbit
494
123
2
2
1
Clonality
Polyclonal
Conjugate
This MAP2K1 antibody is un-conjugated
14
14
14
11
9
8
8
8
6
6
6
6
6
6
6
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
454
208
157
116
94
66
23
22
14
10
9
6
6
2
2
2
2
2
1
Supplier
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Catalog No. ABIN498721
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.34288716 ABIN498700 IF IHC (p) WB Rabbit pThr292 Log in to see Polyclonal
0.34288716 ABIN498855 IHC (p) Rabbit pSer222 Log in to see Polyclonal
0.34288716 ABIN498720 IHC (p) WB Rabbit pSer218, pSer222 Log in to see Polyclonal
0.34288716 ABIN498854 IHC (p) Rabbit pSer218 Log in to see Polyclonal
0.34288716 ABIN498600 IHC (p) WB Rabbit Log in to see Polyclonal
0.34288716 ABIN498654 IHC (p) WB Rabbit Log in to see Polyclonal
0.34288716 ABIN498943 IHC (p) Rabbit Log in to see Polyclonal
0.34288716 ABIN498942 IHC (p) Rabbit Log in to see Polyclonal
0.34288716 ABIN615073 IF IHC (p) WB Rabbit N-Term Log in to see Polyclonal
0.0008982679 ABIN686482 IF (p) IHC (p) WB Rabbit IgG AA 2-35 Log in to see Polyclonal
0.0008982679 ABIN196944 IHC (p) WB Rabbit pSer218, pSer221 Log in to see Polyclonal 2
0.0008982679 ABIN196888 IF IHC (p) WB Rabbit pSer221, pSer222 Log in to see Polyclonal 5
0.0008982679 ABIN272034 IHC (p) WB Rabbit Log in to see Polyclonal
0.0008982679 ABIN3044391 ICC IHC (p) WB Rabbit IgG AA 353-367, C-Term Log in to see Polyclonal
0.0008982679 ABIN317881 IHC (p) WB Rabbit Log in to see Polyclonal
0.0008982679 ABIN197430 IF IHC (p) WB Rabbit Ser217 Log in to see Polyclonal
0.0008982679 ABIN801708 IF (p) IHC (p) WB Rabbit IgG pThr386 Log in to see Polyclonal
0.0008982679 ABIN447144 IHC (p) IHC Rabbit Ser217 Log in to see Polyclonal
0.0008982679 ABIN686484 IHC (p) WB Biotin Rabbit IgG AA 2-35 Log in to see Polyclonal
0.0008982679 ABIN686491 IHC (p) WB HRP Rabbit IgG AA 2-35 Log in to see Polyclonal

Top referenced anti-MAP2K1 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Similar anti-MAP2K1 Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus)
Western Blotting (WB) 442 Antibodies 232 Antibodies 206 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Luminex Assay (LMNX) 10 Antibodies
Intracellular Staining (ICS) 9 Antibodies 9 Antibodies 3 Antibodies
Immunoprecipitation (IP) 14 Antibodies 10 Antibodies 8 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 96 Antibodies 49 Antibodies 44 Antibodies
Immunohistochemistry (IHC) 209 Antibodies 118 Antibodies 111 Antibodies

General

Antigen Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) Antibodies
Epitope pSer222, pSer226
(43), (38), (34), (32), (32), (32), (30), (27), (25), (22), (21), (15), (15), (13), (13), (13), (11), (9), (7), (7), (6), (6), (6), (6), (5), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(606), (323), (280), (60), (38), (24), (15), (14), (13), (6), (4), (4), (4), (2), (2)
Host Rabbit
(494), (123), (2), (2), (1)
Clonality
Polyclonal
Conjugate This MAP2K1 antibody is un-conjugated
(14), (14), (14), (11), (9), (8), (8), (8), (6), (6), (6), (6), (6), (6), (6), (1), (1)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(454), (208), (157), (116), (94), (66), (23), (22), (14), (10), (9), (6), (6), (2), (2), (2), (2), (2), (1)
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Product Details anti-MAP2K1 Antibody

Target Details MAP2K1 Application Details Handling Images
Specificity This antibody detects endogenous levels of MEK-1/2 protein only when phosphorylated at Ser222/226.
Cross-Reactivity (Details) Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
Also reacts to: MAP2K2 pSer226, pSer222
Purification Affinity chromatography
Purity > 95 % by SDS-PAGE

Target Details MAP2K1

Product Details anti-MAP2K1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name MAP2K1 (MAP2K1 Antibody Abstract)
Background A family of protein kinases located upstream of the MAP kinases and responsible for their activation has been identified. The prototype member of this family, designated MAP kinase kinase, or MEK-1, specifically phosphorylates the MAP kinase regulatory threonine and tyrosine residues present in the Thr-Glu-Tyr motif of ERK). A second MEK family member, MEK-2, resembles MEK-1 in its substrate specificity. MEK-3 (or MKK-3) functions to activate p38 MAP kinase, and MEK-4 (also called SEK1 or MKK-4) activates both p38 and JNK MAP kinases. MEK-5 appears to specifically phosphorylate ERK5, whereas MEK-6 phosphorylates p38 and p38beta. MEK-7 (or MKK-7) phosphorylates and activates the JNK signal transduction pathway. Phosphorylation on Ser/Thr by MAP kinase kinase kinases (RAF or MEKK1) positively regulates the kinase activity.Synonyms: Dual specificity mitogen-activated protein kinase kinase 1, ERK activator kinase 1, MAP kinase kinase 1, MAPK/ERK kinase 1, MAPKK 1, MEK1, PRKMK1
Molecular Weight approx. 43 kDa
Gene ID 5604
NCBI Accession NP_002746
UniProt Q02750
Research Area Kinases/Phosphatases, Phospho-specific antibodies
Pathways MAPK Signaling, RTK Signaling, TCR Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-MAP2K1 Antibody Target Details MAP2K1 Handling Images back to top
Application Notes Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-MAP2K1 Antibody Target Details MAP2K1 Application Details Images back to top
Concentration 1,0 mg/mL
Buffer Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Images

Product Details anti-MAP2K1 Antibody Target Details MAP2K1 Application Details Handling back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-MAP2K1 antibody (Mitogen-Activated Protein Kinase Kinase 1) (pSer222) (ABIN498721) Immunohistochemistry (IHC) analyzes of p-MEK-1/2 antibody in paraffin-embedded...
Western Blotting (WB) image for anti-MAP2K1 antibody (Mitogen-Activated Protein Kinase Kinase 1) (pSer222) (ABIN498721) Western blot (WB) analyzes of p-MEK-1/2 antibody in extracts from Jurkat EGF c...