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|Application / Reactivity||Human||Mouse (Murine)||Rat (Rattus)|
|Western Blotting (WB)||442 Antibodies||232 Antibodies||206 Antibodies|
|Proximity Ligation Assay (PLA)||2 Antibodies|
|Luminex Assay (LMNX)||10 Antibodies|
|Intracellular Staining (ICS)||9 Antibodies||9 Antibodies||3 Antibodies|
|Immunoprecipitation (IP)||14 Antibodies||10 Antibodies||8 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||96 Antibodies||49 Antibodies||44 Antibodies|
|Immunohistochemistry (IHC)||209 Antibodies||118 Antibodies||111 Antibodies|
|Antigen||Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) Antibodies|
|Epitope||pSer222, pSer226 Alternatives|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This MAP2K1 antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-MAP2K1 AntibodyTarget Details MAP2K1 Application Details Handling Images
|Specificity||This antibody detects endogenous levels of MEK-1/2 protein only when phosphorylated at Ser222/226.|
Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
Also reacts to: MAP2K2 pSer226, pSer222
|Purity||> 95 % by SDS-PAGE|
Target Details MAP2K1Product Details anti-MAP2K1 Antibody Application Details Handling Images back to top
|Alternative Name||MAP2K1 (MAP2K1 Antibody Abstract)|
|Background||A family of protein kinases located upstream of the MAP kinases and responsible for their activation has been identified. The prototype member of this family, designated MAP kinase kinase, or MEK-1, specifically phosphorylates the MAP kinase regulatory threonine and tyrosine residues present in the Thr-Glu-Tyr motif of ERK). A second MEK family member, MEK-2, resembles MEK-1 in its substrate specificity. MEK-3 (or MKK-3) functions to activate p38 MAP kinase, and MEK-4 (also called SEK1 or MKK-4) activates both p38 and JNK MAP kinases. MEK-5 appears to specifically phosphorylate ERK5, whereas MEK-6 phosphorylates p38 and p38beta. MEK-7 (or MKK-7) phosphorylates and activates the JNK signal transduction pathway. Phosphorylation on Ser/Thr by MAP kinase kinase kinases (RAF or MEKK1) positively regulates the kinase activity.Synonyms: Dual specificity mitogen-activated protein kinase kinase 1, ERK activator kinase 1, MAP kinase kinase 1, MAPK/ERK kinase 1, MAPKK 1, MEK1, PRKMK1|
|Molecular Weight||approx. 43 kDa|
|Research Area||Kinases/Phosphatases, Phospho-specific antibodies|
|Pathways||MAPK Signaling, RTK Signaling, TCR Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway|
Application DetailsProduct Details anti-MAP2K1 Antibody Target Details MAP2K1 Handling Images back to top
Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-MAP2K1 Antibody Target Details MAP2K1 Application Details Images back to top
|Buffer||Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
ImagesProduct Details anti-MAP2K1 Antibody Target Details MAP2K1 Application Details Handling back to top