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|Application / Reactivity||Rat (Rattus)||Mouse (Murine)|
|BioImaging (BI)||2 Antibodies||2 Antibodies|
|ELISA||45 Antibodies||44 Antibodies|
|Enzyme Immunoassay (EIA)||2 Antibodies||3 Antibodies|
|Immunocytochemistry (ICC)||7 Antibodies||5 Antibodies|
|Immunofluorescence (IF)||50 Antibodies||53 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||55 Antibodies||66 Antibodies|
|Immunohistochemistry (IHC)||110 Antibodies||117 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||43 Antibodies|
|Immunoprecipitation (IP)||8 Antibodies|
|Intracellular Staining (ICS)||3 Antibodies|
|Western Blotting (WB)||205 Antibodies|
|Antigen||Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This MAP2K1 antibody is un-conjugated Alternatives|
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-MAP2K1 AntibodyTarget Details MAP2K1 Application Details Handling Images
|Specificity||This antibody detects endogenous levels of Cdc25A protein only when phosphorylated at Thr292.|
Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
|Purity||> 95 % by SDS-PAGE|
Target Details MAP2K1Product Details anti-MAP2K1 Antibody Application Details Handling Images back to top
|Alternative Name||MAP2K1 (MAP2K1 Antibody Abstract)|
|Background||MEK1 (Mitogen activated protein kinase kinase 1) catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. MEK1 activates ERK1 and ERK2 MAP kinases. Mitogen activated protein kinase kinase 2 (MEK2 or MAPKK2) is a member of a family of tyrosine/threonine protein kinases that activate the ERK1 and 2 and MAPK enzymes by phosphorylating both residues within the threonine/glutamate/tyrosine (TEY) motif in the activation loop. MEK1 and 2 are also activated by dual phosphorylation, which occurs on serine 218 and 222, in the activation loop of the MEK. Threonine 292 of MEK1 is phosphorylated by ERK 2, which serves as a negative feedback loop by suppressing activation of MEK1.Synonyms: Dual specificity mitogen-activated protein kinase kinase 1, ERK activator kinase 1, MAP kinase kinase 1, MAPK/ERK kinase 1, MAPKK 1, MEK1, PRKMK1|
|Molecular Weight||approx. 43 kDa|
|Research Area||Kinases/Phosphatases, Phospho-specific antibodies|
|Pathways||MAPK Signaling, RTK Signaling, TCR Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway|
Application DetailsProduct Details anti-MAP2K1 Antibody Target Details MAP2K1 Handling Images back to top
Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200. Immunofluorescence: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-MAP2K1 Antibody Target Details MAP2K1 Application Details Images back to top
|Buffer||Phosphate buffered saline (PBS), pH 7.2., 15 mM sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
ImagesProduct Details anti-MAP2K1 Antibody Target Details MAP2K1 Application Details Handling back to top