anti-Cow (Bovine) MAP2K2 antibody for Western Blotting

Recommended MAP2K2 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2) Antibodies
  • Prkmk2
  • CFC4
  • MAPKK2
  • MEK2
  • MKK2
  • PRKMK2
  • AA589381
  • MK2
  • ATMKK2
  • F27B13.50
  • F27B13_50
  • MAP KINASE KINASE 1
  • MAP kinase kinase 2
  • MK1
  • mitogen activated protein kinase kinase 2
  • mitogen-activated protein kinase kinase 2
  • Map2k2
  • MAP2K2
  • MKK2
Epitope
C-Term
55
35
20
17
17
14
14
13
12
5
4
4
4
4
3
3
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)
314
149
112
13
11
3
2
2
2
1
Host
Rabbit
211
96
11
1
Clonality
Polyclonal
Conjugate
This MAP2K2 antibody is un-conjugated
16
15
10
7
7
7
5
5
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
Application
Immunohistochemistry (IHC), Western Blotting (WB)
272
94
86
47
46
33
27
17
15
13
11
5
4
2
1
Options
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Catalog No. ABIN2783573
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2783572 IHC WB Rabbit N-Term Log in to see Polyclonal 1

General

Antigen Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2) Antibodies
Epitope C-Term
(55), (35), (20), (17), (17), (14), (14), (13), (12), (5), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)
(314), (149), (112), (13), (11), (3), (2), (2), (2), (1)
Host Rabbit
(211), (96), (11), (1)
Clonality
Polyclonal
Conjugate This MAP2K2 antibody is un-conjugated
(16), (15), (10), (7), (7), (7), (5), (5), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Immunohistochemistry (IHC), Western Blotting (WB)
(272), (94), (86), (47), (46), (33), (27), (17), (15), (13), (11), (5), (4), (2), (1)
Pubmed 1 reference available
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Product Details anti-MAP2K2 Antibody

Target Details MAP2K2 Application Details Handling References for anti-MAP2K2 antibody (ABIN2783573) Images
Blocking Peptide Blocking peptide for this product available: ABIN979767
Sequence IKNPAERADL KMLTNHTFIK RSEVEEVDFA GWLCKTLRLN QPGTPTRTAV
Predicted Reactivity Cow: 93%, Dog: 93%, Guinea Pig: 92%, Horse: 93%, Human: 100%, Mouse: 85%, Pig: 93%, Rat: 92%
Characteristics This is a rabbit polyclonal antibody against MAP2K2. It was validated on Western Blot and immunohistochemistry.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the C terminal region of human MAP2K2

Target Details MAP2K2

Product Details anti-MAP2K2 Antibody Application Details Handling References for anti-MAP2K2 antibody (ABIN2783573) Images back to top
Antigen
Alternative Name MAP2K2 (MAP2K2 Antibody Abstract)
Background MAP2K2 is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is known to play a critical role in mitogen growth factor signal transduction. It phosphorylates and thus activates MAPK1/ERK2 and MAPK2/ERK3. The activation of this kinase itself is dependent on the Ser/Thr phosphorylation by MAP kinase kinase kinases. Mutations in MAP2K2 gene cause cardiofaciocutaneous syndrome (CFC syndrome), a disease characterized by heart defects, mental retardation, and distinctive facial features similar to those found in Noonan syndrome. The inhibition or degradation of this kinase is also found to be involved in the pathogenesis of Yersinia and anthrax. A pseudogene, which is located on chromosome 7, has been identified for this gene.The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is known to play a critical role in mitogen growth factor signal transduction. It phosphorylates and thus activates MAPK1/ERK2 and MAPK2/ERK3. The activation of this kinase itself is dependent on the Ser/Thr phosphorylation by MAP kinase kinase kinases. Mutations in this gene cause cardiofaciocutaneous syndrome (CFC syndrome), a disease characterized by heart defects, mental retardation, and distinctive facial features similar to those found in Noonan syndrome. The inhibition or degradation of this kinase is also found to be involved in the pathogenesis of Yersinia and anthrax. A pseudogene, which is located on chromosome 7, has been identified for this gene. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: MAPKK2, MEK2, MKK2, PRKMK2
Protein Interaction Partner: CCNDBP1, RAF1, SUMO2, UBC, EGFR, IQGAP1, NFE2L2, YWHAB, MAPK1, PEX14, BRAF, ARAF, FAM65B, ZNF207, LIG4, MAPK8, WDR83, Ksr1, COPS5, MAP2K1, MEPCE, GRIN1, CNKSR1, LAMTOR3, GRIN2D, RGS12, CASP9, MAPK3, MAP2K2, DUSP3, Flna,
Protein Size: 400
Molecular Weight 44 kDa
Gene ID 5605
NCBI Accession NM_030662, NP_109587
UniProt P36507
Pathways MAPK Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Signaling of Hepatocyte Growth Factor Receptor

Application Details

Product Details anti-MAP2K2 Antibody Target Details MAP2K2 Handling References for anti-MAP2K2 antibody (ABIN2783573) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 400 AA

Restrictions For Research Use only

Handling

Product Details anti-MAP2K2 Antibody Target Details MAP2K2 Application Details References for anti-MAP2K2 antibody (ABIN2783573) Images back to top
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-MAP2K2 antibody (ABIN2783573)

Product Details anti-MAP2K2 Antibody Target Details MAP2K2 Application Details Handling Images back to top
Background publications

Adjei, Cohen, Franklin, Morris, Wilson, Molina, Hanson, Gore, Chow, Leong, Maloney, Gordon, Simmons, Marlow, Litwiler, Brown, Poch, Kane, Haney, Eckhardt: "Phase I pharmacokinetic and pharmacodynamic study of the oral, small-molecule mitogen-activated protein kinase kinase 1/2 inhibitor AZD6244 (ARRY-142886) in patients with advanced cancers." in: Journal of clinical oncology : official journal of the American Society of Clinical Oncology, Vol. 26, Issue 13, pp. 2139-46, 2008

Images

Product Details anti-MAP2K2 Antibody Target Details MAP2K2 Application Details Handling References for anti-MAP2K2 antibody (ABIN2783573) back to top
Supplier Images
Western Blotting (WB) image for anti-Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2) (C-Term) antibody (ABIN2783573) WB Suggested Anti-MAP2K2 Antibody Titration: 0.2-1 ug/ml
Positive Contro...