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anti-Dog (Canine) MAP2K2 antibody for Western Blotting

Recommended MAP2K2 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2) Antibodies
  • Prkmk2
  • CFC4
  • MAPKK2
  • MEK2
  • MKK2
  • PRKMK2
  • AA589381
  • MK2
  • ATMKK2
  • F27B13.50
  • F27B13_50
  • MAP KINASE KINASE 1
  • MAP kinase kinase 2
  • MK1
  • mitogen activated protein kinase kinase 2
  • mitogen-activated protein kinase kinase 2
  • Map2k2
  • MAP2K2
  • MKK2
Epitope
N-Term
34
22
15
15
15
14
14
11
10
8
8
5
5
4
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
246
112
93
8
5
3
2
1
Host
Rabbit
197
45
10
1
Clonality
Polyclonal
Conjugate
This MAP2K2 antibody is un-conjugated
10
10
9
7
7
7
2
2
2
2
2
2
2
2
2
Application
Immunohistochemistry (IHC), Western Blotting (WB)
202
86
83
45
33
28
23
16
15
12
11
4
2
1
1
Options
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Catalog No. ABIN2783572
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
2.959052 ABIN2783573 IHC WB Rabbit C-Term Log in to see Polyclonal 1
2.959052 ABIN967845 IHC (f) IF IP WB Mouse IgG2a AA 1-110 Log in to see 96-MEK2 5
2.959052 ABIN967846 IHC (f) IF IP WB Mouse IgG2a AA 1-110 Log in to see 96-MEK2 5
2.959052 ABIN126838 EIA WB Mouse IgG1 Log in to see 8-00E-008
2.959052 ABIN4333504 IF IHC WB Mouse IgG2a Log in to see 1A-2
1 ABIN2670889 IF IHC WB Mouse IgG2a Log in to see OTI1A2
1 ABIN2670924 IF WB Mouse IgG2a Log in to see OTI5G8
1 ABIN2670957 IF WB Mouse IgG1 Log in to see OTI5B4

Top referenced anti-MAP2K2 antibody for Western Blotting

Similar anti-MAP2K2 Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus) Dog (Canine) Cow (Bovine)
Western Blotting (WB) 199 Antibodies 87 Antibodies 72 Antibodies 9 Antibodies 2 Antibodies
Immunoprecipitation (IP) 28 Antibodies 14 Antibodies 14 Antibodies 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 32 Antibodies 20 Antibodies 14 Antibodies
Immunohistochemistry (IHC) 87 Antibodies 42 Antibodies 39 Antibodies 2 Antibodies

General

Antigen Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2) Antibodies
Epitope N-Term
(34), (22), (15), (15), (15), (14), (14), (11), (10), (8), (8), (5), (5), (4), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
(246), (112), (93), (8), (5), (3), (2), (1)
Host Rabbit
(197), (45), (10), (1)
Clonality
Polyclonal
Conjugate This MAP2K2 antibody is un-conjugated
(10), (10), (9), (7), (7), (7), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Immunohistochemistry (IHC), Western Blotting (WB)
(202), (86), (83), (45), (33), (28), (23), (16), (15), (12), (11), (4), (2), (1), (1)
Pubmed 1 reference available
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Product Details anti-MAP2K2 Antibody

Target Details MAP2K2 Application Details Handling References for anti-MAP2K2 Antibody (ABIN2783572) Images
Blocking Peptide Blocking peptide for this product available: ABIN979766
Sequence LARRKPVLPA LTINPTIAEG PSPTSEGASE ANLVDLQKKL EELELDEQQK
Predicted Reactivity Mouse : 100 %, Rat : 100 %, Bovine : 90 %, Guinea pig : 90 %
Characteristics This is a rabbit polyclonal antibody against MAP2K2. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human MAP2K2

Target Details MAP2K2

Product Details anti-MAP2K2 Antibody Application Details Handling References for anti-MAP2K2 Antibody (ABIN2783572) Images back to top
Antigen
Alternative Name MAP2K2 (MAP2K2 Antibody Abstract)
Background MAP2K2 is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is known to play a critical role in mitogen growth factor signal transduction. It phosphorylates and thus activates MAPK1/ERK2 and MAPK2/ERK3. The activation of this kinase itself is dependent on the Ser/Thr phosphorylation by MAP kinase kinase kinases. Mutations in MAP2K2 gene cause cardiofaciocutaneous syndrome (CFC syndrome), a disease characterized by heart defects, mental retardation, and distinctive facial features similar to those found in Noonan syndrome. The inhibition or degradation of this kinase is also found to be involved in the pathogenesis of Yersinia and anthrax. A pseudogene, which is located on chromosome 7, has been identified for this gene.The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is known to play a critical role in mitogen growth factor signal transduction. It phosphorylates and thus activates MAPK1/ERK2 and MAPK2/ERK3. The activation of this kinase itself is dependent on the Ser/Thr phosphorylation by MAP kinase kinase kinases. Mutations in this gene cause cardiofaciocutaneous syndrome (CFC syndrome), a disease characterized by heart defects, mental retardation, and distinctive facial features similar to those found in Noonan syndrome. The inhibition or degradation of this kinase is also found to be involved in the pathogenesis of Yersinia and anthrax. A pseudogene, which is located on chromosome 7, has been identified for this gene.
Molecular Weight 44 kDa
Gene ID 5605
NCBI Accession NM_030662, NP_109587
UniProt P36507
Pathways MAPK Signaling, RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-MAP2K2 Antibody Target Details MAP2K2 Handling References for anti-MAP2K2 Antibody (ABIN2783572) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 400 AA

Restrictions For Research Use only

Handling

Product Details anti-MAP2K2 Antibody Target Details MAP2K2 Application Details References for anti-MAP2K2 Antibody (ABIN2783572) Images back to top
Format Liquid
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment For short term use, store at 2-8 °C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-MAP2K2 Antibody (ABIN2783572)

Product Details anti-MAP2K2 Antibody Target Details MAP2K2 Application Details Handling Images back to top
Background publications

Adjei, Cohen, Franklin, Morris, Wilson, Molina, Hanson, Gore, Chow, Leong, Maloney, Gordon, Simmons, Marlow, Litwiler, Brown, Poch, Kane, Haney, Eckhardt: "Phase I pharmacokinetic and pharmacodynamic study of the oral, small-molecule mitogen-activated protein kinase kinase 1/2 inhibitor AZD6244 (ARRY-142886) in patients with advanced cancers." in: Journal of clinical oncology : official journal of the American Society of Clinical Oncology, Vol. 26, Issue 13, pp. 2139-46, 2008

Images

Product Details anti-MAP2K2 Antibody Target Details MAP2K2 Application Details Handling References for anti-MAP2K2 Antibody (ABIN2783572) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-MAP2K2 antibody (Mitogen-Activated Protein Kinase Kinase 2) (N-Term) (ABIN2783572) anti-Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2) (N-Term) antibody
Immunohistochemistry (IHC) image for anti-MAP2K2 antibody (Mitogen-Activated Protein Kinase Kinase 2) (N-Term) (ABIN2783572) anti-Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2) (N-Term) antibody (Image 2)
Western Blotting (WB) image for anti-MAP2K2 antibody (Mitogen-Activated Protein Kinase Kinase 2) (N-Term) (ABIN2783572) WB Suggested Anti-MAP2K2
Antibody Titration: 1 µg/mL
Positive Control: ...