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anti-Mouse (Murine) MAP2K2 antibody for Enzyme Immunoassay

Recommended MAP2K2 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2) Antibodies
  • AA589381
  • ATMKK2
  • CFC4
  • F27B13.50
  • F27B13_50
  • MAP KINASE KINASE 1
  • MAP kinase kinase 2
  • MAPKK2
  • MEK2
  • MK1
  • MK2
  • MKK2
  • PRKMK2
  • Prkmk2
Epitope
Middle Region
34
23
15
15
15
14
14
10
10
8
8
5
5
4
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
241
109
93
9
5
3
2
2
Host
Rabbit
194
44
9
1
Clonality
Polyclonal
Conjugate
This MAP2K2 antibody is un-conjugated
10
10
9
7
7
7
2
2
2
2
2
2
2
2
2
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
198
85
81
44
31
27
23
15
15
11
11
3
2
1
1
Supplier
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Catalog No. ABIN360373
$ 401.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008870071 ABIN126838 EIA WB Mouse IgG1 Log in to see 8-00E-008
0.0008870071 ABIN1108139 EIA IF WB Mouse IgG1 Log in to see 7F5 1
0.0008870071 ABIN318069 EIA IHC (p) IP WB Rabbit pThr394 Log in to see Polyclonal

Top referenced anti-MAP2K2 antibody for Enzyme Immunoassay

Similar anti-MAP2K2 Antibodies

Application / Reactivity Mouse (Murine) Human
ELISA 19 Antibodies 77 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies 4 Antibodies
Flow Cytometry (FACS) 5 Antibodies 15 Antibodies
Immunocytochemistry (ICC) 6 Antibodies
Immunofluorescence (IF) 20 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 11 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 23 Antibodies
Immunohistochemistry (IHC) 41 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 2 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 20 Antibodies
Immunoprecipitation (IP) 14 Antibodies
Western Blotting (WB) 85 Antibodies
Dot Blot (DB) 15 Antibodies

General

Antigen Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2) Antibodies
Epitope Middle Region
(34), (23), (15), (15), (15), (14), (14), (10), (10), (8), (8), (5), (5), (4), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(241), (109), (93), (9), (5), (3), (2), (2)
Host Rabbit
(194), (44), (9), (1)
Clonality
Polyclonal
Conjugate This MAP2K2 antibody is un-conjugated
(10), (10), (9), (7), (7), (7), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(198), (85), (81), (44), (31), (27), (23), (15), (15), (11), (11), (3), (2), (1), (1)
Pubmed 2 references available
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Product Details anti-MAP2K2 Antibody

Target Details MAP2K2 Application Details Handling References for anti-MAP2K2 Antibody (ABIN360373) Images
Specificity This antibody reacts to MEK2 (MAP2K2).
Purification Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the center region of human MAP2K2.
Isotype Ig

Target Details MAP2K2

Product Details anti-MAP2K2 Antibody Application Details Handling References for anti-MAP2K2 Antibody (ABIN360373) Images back to top
Antigen
Alternative Name MAPKK 2 (MAP2K2 Antibody Abstract)
Background MAP2K2 is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is known to play a critical role in mitogen growth factor signal transduction. It phosphorylates and thus activates MAPK1/ERK2 and MAPK2/ERK3. The activation of this kinase itself is dependent on the Ser/Thr phosphorylation by MAP kinase kinase kinases. The inhibition or degradation of this kinase is found to be involved in the pathogenesis of Yersinia and anthrax.Synonyms: Dual specificity mitogen-activated protein kinase kinase 2, ERK activator kinase 2, MAP kinase kinase 2, MAPK/ERK kinase 2, MEK2, MKK2
Gene ID 5605, 9606
UniProt P36507
Research Area Kinases/Phosphatases
Pathways MAPK Signaling, RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-MAP2K2 Antibody Target Details MAP2K2 Handling References for anti-MAP2K2 Antibody (ABIN360373) Images back to top
Application Notes ELISA: 1/1,000. Western blotting: 1/100 - 1/500. Immunohistochemistry: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-MAP2K2 Antibody Target Details MAP2K2 Application Details References for anti-MAP2K2 Antibody (ABIN360373) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-MAP2K2 Antibody (ABIN360373)

Product Details anti-MAP2K2 Antibody Target Details MAP2K2 Application Details Handling Images back to top
Background publications

Burroughs, Oh, Barrett, DiAugustine et al.: "Phosphatidylinositol 3-kinase and mek1/2 are necessary for insulin-like growth factor-I-induced vascular endothelial growth factor synthesis in prostate epithelial cells: a role for hypoxia-inducible ..." in: Molecular cancer research : MCR, Vol. 1, Issue 4, pp. 312-22, 2003

Tran, Maurer, Nagamine: "Stabilization of urokinase and urokinase receptor mRNAs by HuR is linked to its cytoplasmic accumulation induced by activated mitogen-activated protein kinase-activated protein kinase 2." in: Molecular and cellular biology, Vol. 23, Issue 20, pp. 7177-88, 2003

Images

Product Details anti-MAP2K2 Antibody Target Details MAP2K2 Application Details Handling References for anti-MAP2K2 Antibody (ABIN360373) back to top
Supplier Images
 image for anti-MAP2K2 antibody (Mitogen-Activated Protein Kinase Kinase 2) (Middle Region) (ABIN360373) anti-Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2) (Middle Region) antibody
 image for anti-MAP2K2 antibody (Mitogen-Activated Protein Kinase Kinase 2) (Middle Region) (ABIN360373) anti-Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2) (Middle Region) antibody (Image 2)