You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Dog (Canine) MAP2K3 antibody for Western Blotting

Recommended MAP2K3 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase Kinase 3 (MAP2K3) Antibodies
  • Mkk3
  • MAPKK3
  • MEK3
  • MKK3
  • PRKMK3
  • SAPKK-2
  • SAPKK2
  • AW212142
  • Prkmk3
  • mMKK3b
  • map2k3
  • zMKK3
  • MAP kinase activator XMEK3
  • mitogen activated protein kinase kinase 3
  • mitogen-activated protein kinase kinase 3
  • mitogen-activated protein kinase kinase 6
  • map2k3
  • Map2k3
  • MAP2K3
  • map2k6
Epitope
N-Term
33
25
15
14
14
12
11
11
7
7
5
5
4
3
3
3
3
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rat (Rattus), Zebrafish (Danio rerio)
304
153
147
56
40
5
4
3
3
3
3
1
1
1
1
Host
Rabbit
201
103
Clonality
Polyclonal
Conjugate
This MAP2K3 antibody is un-conjugated
8
8
7
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
265
103
101
78
56
26
19
14
8
3
3
1
1
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN2777128
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.0977407 ABIN2777129 WB Rabbit C-Term Log in to see Polyclonal 1
3.0977407 ABIN126840 EIA WB Mouse IgG1 Log in to see 5F7
1 ABIN320697 WB Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN1499397 IF IHC (p) WB Mouse IgG2a Log in to see 4B3
1 ABIN1499410 IF WB Mouse IgG2a Log in to see 2D7
1 ABIN1499411 IF WB Mouse IgG2a Log in to see 3C3
1 ABIN1499398 IF IHC (p) WB Mouse IgG2a Log in to see 1B5
1 ABIN1499412 IF WB Mouse IgG2b Log in to see 3C9
1 ABIN1499413 IF WB Mouse IgG1 Log in to see 1D2
1 ABIN1499419 WB Mouse IgG2a Log in to see 3D10
1 ABIN1499404 IF IHC (p) WB Mouse IgG2b Log in to see 6F8
1 ABIN1499399 IF IHC (p) WB Mouse IgG2b Log in to see 6B12
1 ABIN1499400 IF IHC (p) WB Mouse IgG1 Log in to see 5F2
1 ABIN1499401 IF IHC (p) WB Mouse IgG2a Log in to see 5D3
1 ABIN1499405 IF IHC (p) WB Mouse IgG2a Log in to see 5D7
1 ABIN1499414 IF WB Mouse IgG2b Log in to see 2G8
1 ABIN1499415 IF WB Mouse IgG2b Log in to see 5C11
1 ABIN1499420 WB Mouse IgG2b Log in to see 5C4
1 ABIN1499418 IHC (p) WB Mouse IgG2b Log in to see 5G5
1 ABIN1499403 IF IHC (p) WB Mouse IgG2b Log in to see 5C7

Top referenced anti-MAP2K3 antibody for Western Blotting

Similar anti-MAP2K3 Antibodies

Application / Reactivity Human Rat (Rattus) Mouse (Murine) Zebrafish (Danio rerio) Dog (Canine) Cow (Bovine)
Immunofluorescence (fixed cells) (IF/ICC) 3 Antibodies 1 Antibodies 1 Antibodies 1 Antibodies
Cytometry by Time of Flight (CyTOF) 1 Antibodies
Western Blotting (WB) 265 Antibodies 57 Antibodies 126 Antibodies 117 Antibodies 6 Antibodies 4 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Immunoprecipitation (IP) 14 Antibodies 4 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 56 Antibodies 34 Antibodies 33 Antibodies 14 Antibodies
Immunohistochemistry (IHC) 103 Antibodies

General

Antigen Mitogen-Activated Protein Kinase Kinase 3 (MAP2K3) Antibodies
Epitope N-Term
(33), (25), (15), (14), (14), (12), (11), (11), (7), (7), (5), (5), (4), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rat (Rattus), Zebrafish (Danio rerio)
(304), (153), (147), (56), (40), (5), (4), (3), (3), (3), (3), (1), (1), (1), (1)
Host Rabbit
(201), (103)
Clonality
Polyclonal
Conjugate This MAP2K3 antibody is un-conjugated
(8), (8), (7), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Western Blotting (WB)
(265), (103), (101), (78), (56), (26), (19), (14), (8), (3), (3), (1), (1), (1)
Pubmed 1 reference available
Supplier Log in to see

Product Details anti-MAP2K3 Antibody

Target Details MAP2K3 Application Details Handling References for anti-MAP2K3 Antibody (ABIN2777128) Images
Blocking Peptide Blocking peptide for this product available: ABIN979768
Sequence SKPPAPNPTP PRNLDSRTFI TIGDRNFEVE ADDLVTISEL GRGAYGVVEK
Predicted Reactivity Human : 100 %, Rabbit : 100 %, Dog : 90 %, Guinea pig : 90 %, Horse : 90 %, Chicken : 90 %, Bovine : 80 %, Mouse : 80 %, Rat : 80 %
Characteristics This is a rabbit polyclonal antibody against MAP2K3. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human MAP2K3

Target Details MAP2K3

Product Details anti-MAP2K3 Antibody Application Details Handling References for anti-MAP2K3 Antibody (ABIN2777128) Images back to top
Antigen
Alternative Name MAP2K3 (MAP2K3 Antibody Abstract)
Background MAP2K3 is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis. The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis. Multiple alternatively spliced transcript variants that encode distinct isoforms have been reported for this gene.
Molecular Weight 36 kDa
Gene ID 5606
NCBI Accession NM_002756, NP_002747
Research Area Kinases/Phosphatases
Pathways MAPK Signaling, TLR Signaling, Activation of Innate immune Response

Application Details

Product Details anti-MAP2K3 Antibody Target Details MAP2K3 Handling References for anti-MAP2K3 Antibody (ABIN2777128) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 318 AA

Restrictions For Research Use only

Handling

Product Details anti-MAP2K3 Antibody Target Details MAP2K3 Application Details References for anti-MAP2K3 Antibody (ABIN2777128) Images back to top
Format Liquid
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment For short term use, store at 2-8 °C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-MAP2K3 Antibody (ABIN2777128)

Product Details anti-MAP2K3 Antibody Target Details MAP2K3 Application Details Handling Images back to top
Background publications

Prickett, Brautigan: "Cytokine activation of p38 mitogen-activated protein kinase and apoptosis is opposed by alpha-4 targeting of protein phosphatase 2A for site-specific dephosphorylation of MEK3." in: Molecular and cellular biology, Vol. 27, Issue 12, pp. 4217-27, 2007

Images

Product Details anti-MAP2K3 Antibody Target Details MAP2K3 Application Details Handling References for anti-MAP2K3 Antibody (ABIN2777128) back to top
Supplier Images
Western Blotting (WB) image for anti-MAP2K3 antibody (Mitogen-Activated Protein Kinase Kinase 3) (N-Term) (ABIN2777128) anti-Mitogen-Activated Protein Kinase Kinase 3 (MAP2K3) (N-Term) antibody
Immunohistochemistry (IHC) image for anti-MAP2K3 antibody (Mitogen-Activated Protein Kinase Kinase 3) (N-Term) (ABIN2777128) anti-Mitogen-Activated Protein Kinase Kinase 3 (MAP2K3) (N-Term) antibody (Image 2)