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anti-Human MAP2K3 antibody for Enzyme Immunoassay

Recommended MAP2K3 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase Kinase 3 (MAP2K3) Antibodies
  • AW212142
  • map2k3
  • MAPKK3
  • MEK3
  • Mkk3
  • MKK3
  • mMKK3b
  • Prkmk3
  • PRKMK3
  • SAPKK-2
  • SAPKK2
  • zMKK3
Reactivity
Dog (Canine), Human, Mouse (Murine)
297
113
111
27
17
4
2
1
1
Host
Mouse
199
100
Clonality
Monoclonal
Conjugate
This MAP2K3 antibody is un-conjugated
8
8
7
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
253
101
88
79
47
22
20
13
7
2
1
1
1
Supplier
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Catalog No. ABIN126840
$ 533.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008867729 ABIN781846 EIA IHC (p) WB Mouse IgG1 Log in to see 1D10
0.0008867729 ABIN318070 EIA IHC (p) WB Rabbit pSer189 Log in to see Polyclonal

Similar anti-MAP2K3 Antibodies

Application / Reactivity Human Dog (Canine) Mouse (Murine)
Western Blotting (WB) 252 Antibodies 28 Antibodies 87 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Immunoprecipitation (IP) 13 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 47 Antibodies 16 Antibodies
Immunohistochemistry (IHC) 88 Antibodies 42 Antibodies 14 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies 22 Antibodies
Immunofluorescence (IF) 79 Antibodies 20 Antibodies 23 Antibodies
Immunocytochemistry (ICC) 7 Antibodies

General

Antigen Mitogen-Activated Protein Kinase Kinase 3 (MAP2K3) Antibodies
Reactivity Dog (Canine), Human, Mouse (Murine)
(297), (113), (111), (27), (17), (4), (2), (1), (1)
Host Mouse
(199), (100)
Clonality (Clone)
Monoclonal   ( )
Conjugate This MAP2K3 antibody is un-conjugated
(8), (8), (7), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(253), (101), (88), (79), (47), (22), (20), (13), (7), (2), (1), (1), (1)
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Product Details anti-MAP2K3 Antibody

Target Details MAP2K3 Application Details Handling Images
Specificity This antibody specifically recognizes the N-terminus of MKK3.
Characteristics Synonyms: MAPKK 3, MEK3, MKK3, MAP kinase kinase 3, Dual specificity mitogen-activated proteinkinase kinase 3
Purification Size exclusion chromatography
Components incl. positive Control
Immunogen Synthetic peptide conjugated to KLH
Clone 5F7
Isotype IgG1

Target Details MAP2K3

Product Details anti-MAP2K3 Antibody Application Details Handling Images back to top
Antigen
Alternative Name MAP2K3 (MAP2K3 Antibody Abstract)
Background MKK3 activates p38 MAP kinase by phosphorylating a Thr and Tyr residue in the activation loop. MKK3 does not phosphorylate and activate the other major MAP kinases, MAPK1/2 (erk1/2) or SAPK/JNK. Cellular stress and inflammatory cytokines activate MKK3 and lead to phosphorylation of Ser 189 and Thr 193.Synonyms: Dual specificity mitogen-activated protein kinase kinase 3, MAP kinase kinase 3, MAPKK 3, MEK3, MKK3
Gene ID 5606
UniProt P46734
Research Area Kinases/Phosphatases
Pathways MAPK Signaling, TLR Signaling

Application Details

Product Details anti-MAP2K3 Antibody Target Details MAP2K3 Handling Images back to top
Application Notes Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. Positive Control: Cell lysate from untreated A431 cells. ELISA: 0.05 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-MAP2K3 Antibody Target Details MAP2K3 Application Details Images back to top
Reconstitution Restore with 1 mL H2O (15 min, RT).
Buffer 1 mL2 x PBS / 0.09 % Na-azide / PEG and Sucrose
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C/-80 °C
Storage Comment Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch.
Expiry Date 12 months

Images

Product Details anti-MAP2K3 Antibody Target Details MAP2K3 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-MAP2K3 antibody (Mitogen-Activated Protein Kinase Kinase 3) (ABIN126840) anti-Mitogen-Activated Protein Kinase Kinase 3 (MAP2K3) antibody