You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Human MAP2K3 antibody for Immunohistochemistry

Recommended MAP2K3 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase Kinase 3 (MAP2K3) Antibodies
  • Mkk3
  • MAPKK3
  • MEK3
  • MKK3
  • PRKMK3
  • SAPKK-2
  • SAPKK2
  • AW212142
  • Prkmk3
  • mMKK3b
  • map2k3
  • zMKK3
  • MAP kinase activator XMEK3
  • mitogen activated protein kinase kinase 3
  • mitogen-activated protein kinase kinase 3
  • mitogen-activated protein kinase kinase 6
  • map2k3
  • Map2k3
  • MAP2K3
  • map2k6
Epitope
pThr222
31
23
15
14
14
11
11
11
7
7
5
4
3
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
296
148
140
57
40
6
4
4
3
3
3
1
1
1
1
Host
Rabbit
193
103
Clonality
Polyclonal
Conjugate
This MAP2K3 antibody is un-conjugated
8
8
7
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
257
97
95
76
55
26
19
15
8
3
3
1
1
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN1532059
$ 302.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
9.145154 ABIN1532939 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
9.145154 ABIN1870347 IF IHC WB Rabbit IgG pSer189 Log in to see Polyclonal
9.145154 ABIN257414 ICC IF IHC (p) IHC WB Rabbit pSer189 Log in to see Polyclonal
9.145154 ABIN2471270 IHC ELISA WB Mouse IgG1 kappa Log in to see 1D10
9.145154 ABIN2428525 IHC ELISA Rabbit IgG Log in to see Polyclonal
9.145154 ABIN3020316 IF IHC WB Rabbit pSer189 Log in to see Polyclonal
9.145154 ABIN4334800 CyTOF ELISA FACS ICC IF IHC IHC (p) WB Mouse Log in to see 2E12D11
9.145154 ABIN1847502 IHC ELISA WB Rabbit Internal Region, pSer218 Log in to see Polyclonal
9.145154 ABIN1848130 IHC ELISA WB Rabbit Internal Region, Ser207 Log in to see Polyclonal
1 ABIN362698 IF IHC WB Rabbit IgG AA 187-191 Log in to see Polyclonal 2
1 ABIN362138 IF IHC WB Rabbit IgG pSer189 Log in to see Polyclonal 2
1 ABIN3182069 ELISA IHC WB Rabbit IgG pSer218 Log in to see Polyclonal
1 ABIN2568340 IHC WB Rabbit IgG pThr222 Log in to see Polyclonal
1 ABIN2737130 IHC WB Rabbit IgG Log in to see Polyclonal
1 ABIN4880849 ELISA FACS ICC IHC WB Mouse IgG1 AA 1-138 Log in to see 2E12D11
1 ABIN407737 IHC (p) IHC WB Rabbit IgG Log in to see Polyclonal
1 ABIN966578 IHC Rabbit pSer189 Log in to see 2
1 ABIN1584757 IHC ELISA WB Rabbit Middle Region Log in to see Polyclonal
1 ABIN1450377 IHC ELISA WB Rabbit IgG pSer189, Internal Region Log in to see Polyclonal
1 ABIN3182669 ELISA IHC WB Rabbit IgG pThr222 Log in to see Polyclonal

Top referenced anti-MAP2K3 antibody for Immunohistochemistry

  • Human Polyclonal MAP2K3 Primary Antibody for IHC - ABIN966579 (2 Publications): Wang, Chen, Liao, Deng, Zhou, Huang, Sun: Sequential activation of the MEK-extracellular signal-regulated kinase and MKK3/6-p38 mitogen-activated protein kinase pathways mediates oncogenic ras-induced premature senescence. in Molecular and cellular biology 2002 (PubMed)

  • Human MAP2K3 Primary Antibody for IHC - ABIN966578 (2 Publications): Raingeaud, Whitmarsh, Barrett, Dérijard, Davis: MKK3- and MKK6-regulated gene expression is mediated by the p38 mitogen-activated protein kinase signal transduction pathway. in Molecular and cellular biology 1996 (PubMed)

Similar anti-MAP2K3 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine) Human
ELISA 32 Antibodies 41 Antibodies
Immunocytochemistry (ICC) 5 Antibodies 1 Antibodies
Immunofluorescence (IF) 34 Antibodies 36 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 1 Antibodies 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 26 Antibodies 26 Antibodies
Immunohistochemistry (IHC) 52 Antibodies 43 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 33 Antibodies 32 Antibodies
Immunoprecipitation (IP) 4 Antibodies
Western Blotting (WB) 110 Antibodies 121 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Immunochromatography (IC) 1 Antibodies
Cytometry by Time of Flight (CyTOF) 1 Antibodies

General

Antigen Mitogen-Activated Protein Kinase Kinase 3 (MAP2K3) Antibodies
Epitope pThr222
(31), (23), (15), (14), (14), (11), (11), (11), (7), (7), (5), (4), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(296), (148), (140), (57), (40), (6), (4), (4), (3), (3), (3), (1), (1), (1), (1)
Host Rabbit
(193), (103)
Clonality
Polyclonal
Conjugate This MAP2K3 antibody is un-conjugated
(8), (8), (7), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(257), (97), (95), (76), (55), (26), (19), (15), (8), (3), (3), (1), (1), (1)
Pubmed 1 reference available
Supplier Log in to see

Product Details anti-MAP2K3 Antibody

Target Details MAP2K3 Application Details Handling References for anti-MAP2K3 Antibody (ABIN1532059) Images
Specificity MAP2K3 (Phospho-Thr222) Antibody detects endogenous levels of MAP2K3 only when phosphorylated at Thr222.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
Purity > 95 %
Immunogen The antiserum was produced against synthesized peptide derived from human MAP2K3 around the phosphorylation site of Thr222.
Isotype IgG

Target Details MAP2K3

Product Details anti-MAP2K3 Antibody Application Details Handling References for anti-MAP2K3 Antibody (ABIN1532059) Images back to top
Antigen
Alternative Name MAP2K3 (MAP2K3 Antibody Abstract)
Background Molecular weight of target protein: 39 kDa
Synonyms: dual specificity mitogen-activated protein kinase kinase 3, kinase MKK3, MAP kinase kinase 3, MAP2K3, MAPK/ERK kinase 3, MAPKK 3, MEK3, MP2K3, PRKMK3
Molecular Weight 39 kDa
Gene ID 5606
OMIM 602315
UniProt P46734
Research Area Kinases/Phosphatases
Pathways MAPK Signaling, TLR Signaling, Activation of Innate immune Response, Toll-Like Receptors Cascades

Application Details

Product Details anti-MAP2K3 Antibody Target Details MAP2K3 Handling References for anti-MAP2K3 Antibody (ABIN1532059) Images back to top
Application Notes WB: 1:500-1:1000
IHC: 1:50-1:100
ELISA: 1:1000
Optimal working dilution should be determined by the investigator.
Comment

Unigene-Number: Hs.514012 (NCBI Gene Symbol: MAP2K3)

Restrictions For Research Use only

Handling

Product Details anti-MAP2K3 Antibody Target Details MAP2K3 Application Details References for anti-MAP2K3 Antibody (ABIN1532059) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 52 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Stable at -20 °C for at least 1 year.
Expiry Date 12 months

References for anti-MAP2K3 Antibody (ABIN1532059)

Product Details anti-MAP2K3 Antibody Target Details MAP2K3 Application Details Handling Images back to top
Background publications

Dérijard, Raingeaud, Barrett, Wu, Han, Ulevitch, Davis: "Independent human MAP-kinase signal transduction pathways defined by MEK and MKK isoforms." in: Science (New York, N.Y.), Vol. 267, Issue 5198, pp. 682-5, 1995

Images

Product Details anti-MAP2K3 Antibody Target Details MAP2K3 Application Details Handling References for anti-MAP2K3 Antibody (ABIN1532059) back to top
Supplier Images
Western Blotting (WB) image for anti-MAP2K3 antibody (Mitogen-Activated Protein Kinase Kinase 3) (pThr222) (ABIN1532059) Western blot analysis of extracts from Jurkat cells treated with serum 20% 15', using...
Immunohistochemistry (IHC) image for anti-MAP2K3 antibody (Mitogen-Activated Protein Kinase Kinase 3) (pThr222) (ABIN1532059) Immunohistochemistry analysis of paraffin-embedded human brain, using MAP2K3 (Phospho...