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Mouse (Murine) MAP2K3 ELISA Kit

Recommended MAP2K3 Product (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase Kinase 3 (MAP2K3) ELISA Kits
  • Mkk3
  • MAPKK3
  • MEK3
  • MKK3
  • PRKMK3
  • SAPKK-2
  • SAPKK2
  • AW212142
  • Prkmk3
  • mMKK3b
  • map2k3
  • zMKK3
  • MAP kinase activator XMEK3
  • mitogen activated protein kinase kinase 3
  • mitogen-activated protein kinase kinase 3
  • mitogen-activated protein kinase kinase 6
  • map2k3
  • Map2k3
  • MAP2K3
  • map2k6
Epitope
pSer189
Reactivity
Human, Mouse (Murine), Rat (Rattus)
Alternatives
Kits with alternative reactivity to:
9
9
9
Method Type
Sandwich ELISA
Application
ELISA
Options
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Catalog No. ABIN2748360
$ 578.60
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
1 ABIN2748359 Sandwich ELISA Cell Lysate, Tissue Lysate Log in to see
1 ABIN1380776 Cell ELISA Log in to see
1 ABIN1380642 Cell ELISA Log in to see
1 ABIN1381319 Cell ELISA Log in to see
1 ABIN4881290 Cell ELISA Cell Culture Cells Log in to see
1 ABIN2113739 Cell ELISA Log in to see
1 ABIN2113738 Cell ELISA Log in to see
1 ABIN2113737 Cell ELISA Log in to see
1 ABIN2684908 Cell ELISA Cell Samples, Suspension Cell Culture Log in to see

Similar MAP2K3 ELISA Kits

Application / Reactivity Human Mouse (Murine) Rat (Rattus)
ELISA 10 ELISA Kits 10 ELISA Kits 10 ELISA Kits

General

Antigen Mitogen-Activated Protein Kinase Kinase 3 (MAP2K3) ELISA Kits
Epitope pSer189
Reactivity Human, Mouse (Murine), Rat (Rattus)
Kits with alternative reactivity to:
(9), (9), (9)
Method Type Sandwich ELISA
Application ELISA
Supplier Log in to see

Product Details MAP2K3 ELISA Kit

Target details Application Details Handling Images
Purpose Human/Mouse/Rat Phospho-MKK3 (S189) ELISA Kit. This assay semi-quantitatively measures phophorylated MKK3 (Ser189) in lysate samples.
Sample Type Cell Lysate, Tissue Lysate
Analytical Method Semi-Quantitative
Specificity The antibody pair provided in this kit recognizes human, mouse, and rat PEL phosphorylated at site Serine-189.
Characteristics
  • Rapidly measure phosphorylated protein in lysates
  • Screen numerous different cell lysates without performing a Western Blot analysis
  • Minimal hands-on time, convenient, and non-radioactive material
Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Anti-Phospho Antibody
  • HRP-Conjugated Secondary Antibody
  • Assay Diluent
  • TMB One-Step Substrate
  • Stop Solution
  • Lysis Buffer
  • Positive Control Sample
Material not included
  • Distilled or deionized water
  • 100 mL and 1 liter graduated cylinders
  • Tubes to prepare sample dilutions
  • Protease and Phosphatase inhibitors
  • Precision pipettes to deliver 2 μL to 1 mL volumes
  • Adjustable 1-25 mL pipettes for reagent preparation
  • Benchtop rocker or shaker
  • Microplate reader capable of measuring absorbance at 450 nm

Target details

Product Details MAP2K3 ELISA Kit Application Details Handling Images back to top
Antigen
Alternative Name MKK3 (MAP2K3 ELISA Kit Abstract)
Background Mitogen-activated protein kinase kinase 3(MKK3) phosphorylated at Serine-189
Gene ID 5606
UniProt P46734
Research Area Kinases/Phosphatases
Pathways MAPK Signaling, TLR Signaling, Activation of Innate immune Response, Toll-Like Receptors Cascades, Autophagy, Signaling Events mediated by VEGFR1 and VEGFR2

Application Details

Product Details MAP2K3 ELISA Kit Target details Handling Images back to top
Plate Pre-coated,Strips (12 x 8)
Protocol
  1. Prepare all reagents and samples as instructed in the manual.
  2. Add 100 μL of sample or positive control to each well.
  3. Incubate 2.5 h at RT or O/N at 4 °C.
  4. Add 100 μL of prepared primary antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
  7. Incubate 1 h at RT.
  8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 μL of Stop Solution to each well.
  11. Read at 450 nm immediately.
Assay Procedure Prepare all reagents and samples as instructed in the manual.
Add 100 μL of sample or positive control to each well.
Incubate 2.5 h at RT or O/N at 4 °C.
Add 100 μL of prepared primary antibody to each well.
Incubate 1 h at RT.
Add 100 μL of prepared 1X HRP-Streptavidin to each well.
Incubate 1 h at RT.
Add 100 μL of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 μL of Stop Solution to each well.
Read at 450 nm immediately.
Restrictions For Research Use only

Handling

Product Details MAP2K3 ELISA Kit Target details Application Details Images back to top
Storage -20 °C
Storage Comment Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
Expiry Date 6 months