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anti-Human MAP2K4 antibody for Enzyme Immunoassay

Recommended MAP2K4 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase Kinase 4 (MAP2K4) Antibodies
  • 2
  • 142758_at
  • ARABIDOPSIS THALIANA MITOGEN-ACTIVATED PROTEIN KINASE KINASE 4
  • ATMEK4
  • ATMKK4
  • CG9738
  • CT27508
  • CT38386
  • D-MKK4
  • DmelCG12798
  • Dmel\\CG9738
  • dMkk4
  • DMKK4
  • dMKK4
  • F19C24.26
  • F19C24_26
  • hep
  • jnkk
  • JNKK
  • JNKK1
  • jnkk1
  • Jnkk2
  • JNKK2
  • map2k4
  • MAP KINASE KINASE 4
  • MAPKK4
  • mapkk4
  • Mapkk7
  • MEK4
  • mek4
  • Mek7
  • mitogen-activated protein kinase kinase 4
  • MKK4
  • mkk4
  • MKK4A-l
  • Mkk7
  • Mpk4
  • PRKMK4
  • prkmk4
  • SAPKK-1
  • SAPKK1
  • Sek1
  • sek1
  • SEK1
  • SEK1/MKK4
  • serk1
  • SERK1
  • Serk1
  • SKK1
  • xmek2
  • zgc:136500
Epitope
Ser257
55
41
28
24
21
19
15
13
11
5
5
4
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
292
137
107
9
8
4
4
2
Host
Rabbit
271
34
Clonality
Polyclonal
Conjugate
This MAP2K4 antibody is un-conjugated
12
12
11
8
8
8
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
197
140
112
58
32
17
14
13
8
6
3
3
1
Supplier
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Catalog No. ABIN360303
$ 401.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008833851 ABIN1108124 EIA IHC (p) Mouse IgM Log in to see 2D10D8 3
0.0008833851 ABIN271811 EIA WB Rabbit Log in to see Polyclonal
0.0008833851 ABIN1108125 EIA IHC (p) WB Mouse IgG1 Log in to see 5H4

Top referenced anti-MAP2K4 antibody for Enzyme Immunoassay

Similar anti-MAP2K4 Antibodies

Application / Reactivity Human
Dot Blot (DB) 14 Antibodies
ELISA 108 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies
Flow Cytometry (FACS) 8 Antibodies
Immunochromatography (IC) 3 Antibodies
Immunocytochemistry (ICC) 6 Antibodies
Immunofluorescence (IF) 58 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13 Antibodies
Immunohistochemistry (IHC) 141 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 34 Antibodies
Immunoprecipitation (IP) 17 Antibodies
Western Blotting (WB) 191 Antibodies

General

Antigen Mitogen-Activated Protein Kinase Kinase 4 (MAP2K4) Antibodies
Epitope Ser257
(55), (41), (28), (24), (21), (19), (15), (13), (11), (5), (5), (4), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(292), (137), (107), (9), (8), (4), (4), (2)
Host Rabbit
(271), (34)
Clonality
Polyclonal
Conjugate This MAP2K4 antibody is un-conjugated
(12), (12), (11), (8), (8), (8), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(197), (140), (112), (58), (32), (17), (14), (13), (8), (6), (3), (3), (1)
Pubmed 3 references available
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Product Details anti-MAP2K4 Antibody

Target Details MAP2K4 Application Details Handling References for anti-MAP2K4 Antibody (ABIN360303) Images
Specificity This antibody reacts to MEKK4 (MAP2K4).
Purification Protein A column, followed by peptide affinity purificatio
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding S257of human MAP2K4.
Isotype Ig

Target Details MAP2K4

Product Details anti-MAP2K4 Antibody Application Details Handling References for anti-MAP2K4 Antibody (ABIN360303) Images back to top
Antigen
Alternative Name MAP2K4 (MAP2K4 Antibody Abstract)
Background MAP2K4 is a dual specificity protein kinase that belongs to the Ser/Thr protein kinase family. This kinase is a direct activator of MAP kinases in response to various environmental stresses or mitogenic stimuli. It has been shown to activate MAPK8/JNK1, MAPK9/JNK2, and MAPK14/p38, but not MAPK1/ERK2 or MAPK3/ERK3. MAP2K4 is phosphorylated, and thus activated by MAP3K1/MEKK. The knockout studies in mice suggested the roles of this kinase in mediating survival signal in T cell development, as well as in the organogenesis of liver.Synonyms: Dual specificity mitogen-activated protein kinase kinase 4, JNK-activating kinase 1, MAP Kinase Kinase 4, MAPKK4, MEK-4, MKK4, PRKMK4, SAPK/ERK kinase 1, SERK1, c-Jun N-terminal kinase kinase 1
Gene ID 6416, 9606
UniProt P45985
Pathways MAPK Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway

Application Details

Product Details anti-MAP2K4 Antibody Target Details MAP2K4 Handling References for anti-MAP2K4 Antibody (ABIN360303) Images back to top
Application Notes ELISA: 1/1,000. Western blotting: 1/50 - 1/100. Immunohistochemistry: 1/10 - 1/50.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-MAP2K4 Antibody Target Details MAP2K4 Application Details References for anti-MAP2K4 Antibody (ABIN360303) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-MAP2K4 Antibody (ABIN360303)

Product Details anti-MAP2K4 Antibody Target Details MAP2K4 Application Details Handling Images back to top
Background publications

Robinson, Shalhav, Otto, Kawai, Gorospe, Rinker-Schaeffer: "Mitogen-activated protein kinase kinase 4/c-Jun NH2-terminal kinase kinase 1 protein expression is subject to translational regulation in prostate cancer cell lines." in: Molecular cancer research : MCR, Vol. 6, Issue 3, pp. 501-8, 2008

Zhang, Zhang, Zhang, Lin, Li, Pober, Min: "RIP1-mediated AIP1 phosphorylation at a 14-3-3-binding site is critical for tumor necrosis factor-induced ASK1-JNK/p38 activation." in: The Journal of biological chemistry, Vol. 282, Issue 20, pp. 14788-96, 2007

Salmeron, Ahmad, Carlile, Pappin, Narsimhan, Ley: "Activation of MEK-1 and SEK-1 by Tpl-2 proto-oncoprotein, a novel MAP kinase kinase kinase." in: The EMBO journal, Vol. 15, Issue 4, pp. 817-26, 1996

Images

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Supplier Images
 image for anti-MAP2K4 antibody (Mitogen-Activated Protein Kinase Kinase 4) (Ser257) (ABIN360303) anti-Mitogen-Activated Protein Kinase Kinase 4 (MAP2K4) (Ser257) antibody
 image for anti-MAP2K4 antibody (Mitogen-Activated Protein Kinase Kinase 4) (Ser257) (ABIN360303) anti-Mitogen-Activated Protein Kinase Kinase 4 (MAP2K4) (Ser257) antibody (Image 2)