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|Application / Reactivity||Human|
|Dot Blot (DB)||14 Antibodies|
|Enzyme Immunoassay (EIA)||4 Antibodies|
|Flow Cytometry (FACS)||8 Antibodies|
|Immunochromatography (IC)||3 Antibodies|
|Immunocytochemistry (ICC)||6 Antibodies|
|Immunofluorescence (IF)||58 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||17 Antibodies|
|Immunohistochemistry (IHC)||141 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||2 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||34 Antibodies|
|Immunoprecipitation (IP)||17 Antibodies|
|Western Blotting (WB)||191 Antibodies|
|Antigen||Mitogen-Activated Protein Kinase Kinase 4 (MAP2K4) Antibodies|
|Conjugate||This MAP2K4 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|3 references available|
|Supplier||Log in to see|
Product Details anti-MAP2K4 AntibodyTarget Details MAP2K4 Application Details Handling References for anti-MAP2K4 Antibody (ABIN360303) Images
|Specificity||This antibody reacts to MEKK4 (MAP2K4).|
|Purification||Protein A column, followed by peptide affinity purificatio|
|Immunogen||This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding S257of human MAP2K4.|
Target Details MAP2K4Product Details anti-MAP2K4 Antibody Application Details Handling References for anti-MAP2K4 Antibody (ABIN360303) Images back to top
|Alternative Name||MAP2K4 (MAP2K4 Antibody Abstract)|
|Background||MAP2K4 is a dual specificity protein kinase that belongs to the Ser/Thr protein kinase family. This kinase is a direct activator of MAP kinases in response to various environmental stresses or mitogenic stimuli. It has been shown to activate MAPK8/JNK1, MAPK9/JNK2, and MAPK14/p38, but not MAPK1/ERK2 or MAPK3/ERK3. MAP2K4 is phosphorylated, and thus activated by MAP3K1/MEKK. The knockout studies in mice suggested the roles of this kinase in mediating survival signal in T cell development, as well as in the organogenesis of liver.Synonyms: Dual specificity mitogen-activated protein kinase kinase 4, JNK-activating kinase 1, MAP Kinase Kinase 4, MAPKK4, MEK-4, MKK4, PRKMK4, SAPK/ERK kinase 1, SERK1, c-Jun N-terminal kinase kinase 1|
|Gene ID||6416, 9606|
|Pathways||MAPK Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway|
Application DetailsProduct Details anti-MAP2K4 Antibody Target Details MAP2K4 Handling References for anti-MAP2K4 Antibody (ABIN360303) Images back to top
ELISA: 1/1,000. Western blotting: 1/50 - 1/100. Immunohistochemistry: 1/10 - 1/50.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-MAP2K4 Antibody Target Details MAP2K4 Application Details References for anti-MAP2K4 Antibody (ABIN360303) Images back to top
|Buffer||PBS with 0.09 % (W/V) sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.|
References for anti-MAP2K4 Antibody (ABIN360303)Product Details anti-MAP2K4 Antibody Target Details MAP2K4 Application Details Handling Images back to top
Robinson, Shalhav, Otto et al.: "Mitogen-activated protein kinase kinase 4/c-Jun NH2-terminal kinase kinase 1 protein expression is subject to translational regulation in prostate cancer cell lines." in: Molecular cancer research : MCR, Vol. 6, Issue 3, pp. 501-8, 2008 (PubMed).
Zhang, Zhang, Zhang et al.: "RIP1-mediated AIP1 phosphorylation at a 14-3-3-binding site is critical for tumor necrosis factor-induced ASK1-JNK/p38 activation." in: The Journal of biological chemistry, Vol. 282, Issue 20, pp. 14788-96, 2007 (PubMed).
Salmeron, Ahmad, Carlile et al.: "Activation of MEK-1 and SEK-1 by Tpl-2 proto-oncoprotein, a novel MAP kinase kinase kinase." in: The EMBO journal, Vol. 15, Issue 4, pp. 817-26, 1996 (PubMed).
ImagesProduct Details anti-MAP2K4 Antibody Target Details MAP2K4 Application Details Handling References for anti-MAP2K4 Antibody (ABIN360303) back to top