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anti-Human MAP2K5 antibody for Immunofluorescence

Recommended MAP2K5 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase Kinase 5 (MAP2K5) Antibodies
  • AI324775
  • AI428457
  • ARABIDOPSIS THALIANA MITOGEN-ACTIVATED PROTEIN KINASE KINASE 5
  • ATMAP2K_ALPHA
  • ATMEK5
  • ATMKK5
  • fb73f02
  • HsT17454
  • MAP2K_A
  • MAP KINASE KINASE 5
  • MAP kinase kinase 5
  • MAPKK5
  • Mapkk5
  • Mek5
  • MEK5
  • PRKMK5
  • Prkmk5
  • wu:fb73f02
  • zgc:172137
Epitope
AA 13-188
33
32
17
17
15
14
14
14
14
6
5
5
4
4
3
2
2
2
2
1
1
1
1
1
1
Reactivity
Human
158
79
78
11
9
7
6
5
3
3
3
3
2
2
1
Host
Mouse
160
15
Clonality
Monoclonal
Conjugate
This MAP2K5 antibody is un-conjugated
9
9
9
9
5
5
5
5
5
5
5
5
4
4
4
Application
Immunofluorescence (IF), Western Blotting (WB)
110
59
47
29
23
21
4
2
2
1
1
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Catalog No. ABIN968272
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.2735693 ABIN968273 IF WB Mouse IgG1 AA 13-188 Log in to see 21-MEK5 2
0.2735693 ABIN519261 IF WB Mouse AA 1-448, full length Log in to see Polyclonal
0.2735693 ABIN562430 IF PLA ELISA WB Mouse IgG1 kappa AA 1-120, partial Log in to see 1E5
0.2735693 ABIN562431 IF ELISA WB Mouse IgG1 kappa AA 1-120, partial Log in to see 2C2
0.2735693 ABIN562429 IF ELISA WB Mouse IgG1 kappa AA 1-448, full length Log in to see M1-E6
0.2735693 ABIN562432 IF ELISA WB Mouse IgG1 kappa AA 1-120, partial Log in to see 1F6
0.2735693 ABIN562433 IF ELISA WB Mouse IgG1 kappa AA 1-120, partial Log in to see 1B4
0.2735693 ABIN562434 IF ELISA WB Mouse IgG1 kappa AA 1-120, partial Log in to see 3E10
0.2735693 ABIN3198202 IC IF WB Rabbit Log in to see Polyclonal
0.2735693 ABIN4333506 ICC IF IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal
0.0011096579 ABIN393416 IF WB Mouse IgG1 kappa Met11 Log in to see 3E10 5
0.0011096579 ABIN961575 IF ELISA WB Mouse IgG1, kappa Log in to see M1-E6
0.0011096579 ABIN1088849 IF ELISA WB Rabbit IgG Log in to see Polyclonal
0.0011096579 ABIN1088850 IF ELISA WB Rabbit IgG Log in to see Polyclonal
0.0011096579 ABIN2881938 IF Rabbit IgG Log in to see Polyclonal
0.0011096579 ABIN2563757 IF IHC WB Rabbit IgG Log in to see Polyclonal
0.0011096579 ABIN2306855 IF ELISA WB Mouse IgG1 kappa AA 1-120 Log in to see 3E10
0.0011096579 ABIN2306861 IF ELISA WB Mouse IgG1 kappa AA 1-449 Log in to see M1-E6
0.0011096579 ABIN2970174 ICC IF IHC WB Rabbit IgG Log in to see Polyclonal
0.0011096579 ABIN2146187 IF ELISA WB Rabbit IgG Log in to see Polyclonal

Top referenced anti-MAP2K5 antibody for Immunofluorescence

Similar anti-MAP2K5 Antibodies

Application / Reactivity Human
ELISA 46 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 2 Antibodies
Immunofluorescence (IF) 22 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 59 Antibodies
Immunohistochemistry (IHC) 29 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 23 Antibodies
Immunoprecipitation (IP) 4 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Western Blotting (WB) 95 Antibodies

General

Antigen Mitogen-Activated Protein Kinase Kinase 5 (MAP2K5) Antibodies
Epitope AA 13-188
(33), (32), (17), (17), (15), (14), (14), (14), (14), (6), (5), (5), (4), (4), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Human
(158), (79), (78), (11), (9), (7), (6), (5), (3), (3), (3), (3), (2), (2), (1)
Host Mouse
(160), (15)
Clonality (Clone)
Monoclonal   ( )
Conjugate This MAP2K5 antibody is un-conjugated
(9), (9), (9), (9), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (4)
Application Immunofluorescence (IF), Western Blotting (WB)
(110), (59), (47), (29), (23), (21), (4), (2), (2), (1), (1)
Pubmed 2 references available
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Product Details anti-MAP2K5 Antibody

Target Details MAP2K5 Application Details Handling References for anti-MAP2K5 Antibody (ABIN968272) Images
Cross-Reactivity Mouse (Murine), Rat (Rattus), Dog (Canine)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Human MEK5
Clone LPR-01
Isotype IgG1

Target Details MAP2K5

Product Details anti-MAP2K5 Antibody Application Details Handling References for anti-MAP2K5 Antibody (ABIN968272) Images back to top
Antigen
Alternative Name MEK5 (MAP2K5 Antibody Abstract)
Background The mitogen-activated protein (MAP) kinase cascade participates in the Ras signal transduction pathway. The MAP kinase cascade consists of MEK kinase (Raf-1 or B-Raf), MAP/ERK kinase (MEK1 or MEK2), and extracellular-regulated protein kinase (ERK1 or ERK2). Raf becomes activated following its interaction with Ras-GTP (activated Ras). Via phosphorylation reactions, Raf activates MEK which, in turn, activates ERK. MEK5, also known as MAP Kinase 5, is a 50 kDa protein that is 40% identical to MEK1 and MEK2. Alternative splicing results in two MEK5 isoforms: alpha (50 kDa) and beta (40 kDa). MEK5beta has been reported to be ubiquitously expressed while MEK5alpha is expressed primarily in the liver and brain. The N-terminal amino acid sequence of MEK5alpha resembles sequences found in actin cytoskeletal proteins, indicating that MEK5alpha may associate with the cytoskeleton. However, the role of MEK5 in the MAP kinase pathway and its substrate remain to be identified. This antibody is routinely tested by western blot analysis.
Synonyms: MAP Kinase-5
Molecular Weight 50 kDa
Research Area Cancer, Kinases/Phosphatases
Pathways MAPK Signaling, Neurotrophin Signaling Pathway

Application Details

Product Details anti-MAP2K5 Antibody Target Details MAP2K5 Handling References for anti-MAP2K5 Antibody (ABIN968272) Images back to top
Comment

Related Products: ABIN968537, ABIN967389

Restrictions For Research Use only

Handling

Product Details anti-MAP2K5 Antibody Target Details MAP2K5 Application Details References for anti-MAP2K5 Antibody (ABIN968272) Images back to top
Format Liquid
Concentration 250 µg/ml
Buffer Aqueous buffered solution containing BSA, glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C

References for anti-MAP2K5 Antibody (ABIN968272)

Product Details anti-MAP2K5 Antibody Target Details MAP2K5 Application Details Handling Images back to top
Product cited in:

English, Vanderbilt, Xu, Marcus, Cobb: "Isolation of MEK5 and differential expression of alternatively spliced forms." in: The Journal of biological chemistry, Vol. 270, Issue 48, pp. 28897-902, 1996

Zhou, Bao, Dixon: "Components of a new human protein kinase signal transduction pathway." in: The Journal of biological chemistry, Vol. 270, Issue 21, pp. 12665-9, 1995

Images

Product Details anti-MAP2K5 Antibody Target Details MAP2K5 Application Details Handling References for anti-MAP2K5 Antibody (ABIN968272) back to top
Supplier Images
Western Blotting (WB) image for anti-MAP2K5 antibody (Mitogen-Activated Protein Kinase Kinase 5) (AA 13-188) (ABIN968272) Western blot analysis of MEK5 on a Jurkat cell lysate (Human T-cell leukemia, ATCC TI...
Immunofluorescence (IF) image for anti-MAP2K5 antibody (Mitogen-Activated Protein Kinase Kinase 5) (AA 13-188) (ABIN968272) Immunofluorescence staining on human endothelial cells.