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anti-Zebrafish (Danio rerio) MAP2K5 antibody for Western Blotting

Recommended MAP2K5 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase Kinase 5 (MAP2K5) Antibodies
  • Mek5
  • HsT17454
  • MAPKK5
  • MEK5
  • PRKMK5
  • AI324775
  • AI428457
  • Mapkk5
  • Prkmk5
  • fb73f02
  • wu:fb73f02
  • zgc:172137
  • ARABIDOPSIS THALIANA MITOGEN-ACTIVATED PROTEIN KINASE KINASE 5
  • ATMAP2K_ALPHA
  • ATMEK5
  • ATMKK5
  • MAP KINASE KINASE 5
  • MAP kinase kinase 5
  • MAP2K_A
  • mitogen activated protein kinase kinase 5
  • mitogen-activated protein kinase kinase 5
  • Map2k5
  • MAP2K5
  • map2k5
  • MKK5
Epitope
pSer311, pThr315
32
29
15
15
15
15
14
11
10
8
6
6
6
5
4
3
3
2
2
2
2
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Chicken, Human, Mouse (Murine), Non Human Primates, Rat (Rattus), Xenopus laevis, Zebrafish (Danio rerio)
166
87
81
10
6
6
5
4
3
3
3
2
2
2
2
1
1
1
Host
Rabbit
160
16
Clonality
Polyclonal
Conjugate
This MAP2K5 antibody is un-conjugated
9
9
9
5
5
5
5
5
5
5
5
5
5
4
4
4
4
Application
Western Blotting (WB)
108
64
47
28
24
22
4
3
2
1
1
1
Supplier
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Catalog No. ABIN372667
$ 412.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN318850 WB Rabbit IgG pSer311, pThr315 Log in to see Polyclonal 1
1 ABIN2959817 WB Rabbit IgG pSer311, pSer315, pThr315 Log in to see Polyclonal

Top referenced anti-MAP2K5 antibody for Western Blotting

Similar anti-MAP2K5 Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus) Dog (Canine) Chicken Zebrafish (Danio rerio) Cow (Bovine) Xenopus laevis Non Human Primates
Immunofluorescence (fixed cells) (IF/ICC) 3 Antibodies 2 Antibodies 2 Antibodies
Western Blotting (WB) 11 Antibodies 99 Antibodies 51 Antibodies 46 Antibodies 6 Antibodies 3 Antibodies 7 Antibodies 7 Antibodies 1 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Immunoprecipitation (IP) 1 Antibodies 4 Antibodies 1 Antibodies 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 24 Antibodies 11 Antibodies

General

Antigen Mitogen-Activated Protein Kinase Kinase 5 (MAP2K5) Antibodies
Epitope pSer311, pThr315
(32), (29), (15), (15), (15), (15), (14), (11), (10), (8), (6), (6), (6), (5), (4), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Chicken, Human, Mouse (Murine), Non Human Primates, Rat (Rattus), Xenopus laevis, Zebrafish (Danio rerio)
(166), (87), (81), (10), (6), (6), (5), (4), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1)
Host Rabbit
(160), (16)
Clonality
Polyclonal
Conjugate This MAP2K5 antibody is un-conjugated
(9), (9), (9), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (4), (4)
Application Western Blotting (WB)
(108), (64), (47), (28), (24), (22), (4), (3), (2), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-MAP2K5 Antibody

Target Details MAP2K5 Application Details Handling References for anti-MAP2K5 Antibody (ABIN372667) Images
Specificity This antibody is specific for the ~49k MEK5 protein phosphorylated at Ser311, Thr315. Immunolabeling of the MEK5 band is blocked by preadsorption with the phospho-peptide used as antigen but not be the corresponding dephospho-peptide.
Cross-Reactivity (Details) Species reactivity (expected):Mouse, Human, Chicken, Bovine, Canine, non-Human Primates, Xenopus, and Zebrafish.
Species reactivity (tested):Rat.
Purification Sequential Chromatography on phospho- and dephosphopeptide affinity columns.
Immunogen Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser311, Thr315 of MEK5.
Isotype IgG

Target Details MAP2K5

Product Details anti-MAP2K5 Antibody Application Details Handling References for anti-MAP2K5 Antibody (ABIN372667) Images back to top
Antigen
Alternative Name MAP2K5 (MAP2K5 Antibody Abstract)
Background MEK5 (also known as MKK5) is a dual specificity serine/threonine protein kinase belonging to the MAP kinase kinase family. MEK5 has been shown to specifically activate ERK5 (Zhou et al., 1995) whereas MEK5 itself is regulated by MEKK3 (Chao et al., 1999). An important link between MEK5 and metastatic prostrate cancer has been demonstrated (Mehta et.al., 2003). Dual phosphorylation of Ser311 and Thr315 have been implicated in cell proliferation (Cameron et al., 2004).Synonyms: Dual specificity mitogen-activated protein kinase kinase 5, MAP kinase kinase 5, MAPKK 5, MEK5, MKK5
Gene ID 29568
NCBI Accession NP_001029159
UniProt Q62862
Research Area Kinases/Phosphatases
Pathways MAPK Signaling, Neurotrophin Signaling Pathway

Application Details

Product Details anti-MAP2K5 Antibody Target Details MAP2K5 Handling References for anti-MAP2K5 Antibody (ABIN372667) Images back to top
Application Notes Western blot: 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-MAP2K5 Antibody Target Details MAP2K5 Application Details References for anti-MAP2K5 Antibody (ABIN372667) Images back to top
Format Liquid
Buffer 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody undiluted (in aliquots) at-20 °C.

References for anti-MAP2K5 Antibody (ABIN372667)

Product Details anti-MAP2K5 Antibody Target Details MAP2K5 Application Details Handling Images back to top
Background publications

Zhou, Bao, Dixon: "Components of a new human protein kinase signal transduction pathway." in: The Journal of biological chemistry, Vol. 270, Issue 21, pp. 12665-9, 1995

Images

Product Details anti-MAP2K5 Antibody Target Details MAP2K5 Application Details Handling References for anti-MAP2K5 Antibody (ABIN372667) back to top
Supplier Images
 image for anti-MAP2K5 antibody (Mitogen-Activated Protein Kinase Kinase 5) (pSer311) (ABIN372667) anti-Mitogen-Activated Protein Kinase Kinase 5 (MAP2K5) (pSer311), (pThr315) antibody