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|Application / Reactivity||Human||Mouse (Murine)||Rat (Rattus)|
|Immunofluorescence (fixed cells) (IF/ICC)||6 Antibodies||4 Antibodies||4 Antibodies|
|Western Blotting (WB)||154 Antibodies||75 Antibodies||103 Antibodies|
|Proximity Ligation Assay (PLA)||2 Antibodies|
|Immunoprecipitation (IP)||17 Antibodies||5 Antibodies||5 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||33 Antibodies||22 Antibodies||29 Antibodies|
|Immunohistochemistry (IHC)||75 Antibodies||45 Antibodies||38 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||27 Antibodies||27 Antibodies||27 Antibodies|
|Antigen||Mitogen-Activated Protein Kinase Kinase 6 (MAP2K6) Antibodies|
|Epitope||AA 7-23, N-Term Alternatives|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This MAP2K6 antibody is un-conjugated Alternatives|
Western Blotting (WB)
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Product Details anti-MAP2K6 AntibodyTarget Details MAP2K6 Application Details Handling Images
|Cross-Reactivity (Details)||No cross reactivity with other proteins.|
Rabbit IgG polyclonal antibody for Dual specificity mitogen-activated protein kinase kinase 6(MAP2K6) detection. Tested with WB in Human,Mouse,Rat.
Gene Name: mitogen-activated protein kinase kinase 6
Protein Name: Dual specificity mitogen-activated protein kinase kinase 6
|Purification||Immunogen affinity purified.|
|Immunogen||A synthetic peptide corresponding to a sequence at the N-terminus of human MEK6(7-23aa KKRNPGLKIPKEAFEQP), identical to the related mouse sequence and different from the related rat sequence by one amino acid.|
Target Details MAP2K6Product Details anti-MAP2K6 Antibody Application Details Handling Images back to top
|Alternative Name||MAP2K6 (MAP2K6 Antibody Abstract)|
|Background||MAP2K6(Mitogen-activated protein kinase kinase 6), also known as MAP kinase kinase 6(MAPKK 6) or MAPK/ERK kinase 6 is an enzyme that in humans is encoded by the MAP2K6 gene, on chromosome 17. MAPKK 6 is a member of the dual specificity protein kinase family, which functions as a mitogen-activated protein(MAP) kinase kinase. Han et al.(1996) described experiments addressing the function of MKK6 in intracellular signaling pathways leading toward activation of p38 MAPK. Mukherjee et al.(2006) found that the Yersinia YopJ virulence factor acted as an acetyltransferase, using coenzyme A to modify critical ser and thr residues in the MAP2K6 activation loop, thereby blocking phosphorylation and kinase activation. They proposed that ser and/or thr acetylation by a bacterial effector may be a mechanism that occurs in other signaling pathways.|
|Pathways||MAPK Signaling, TLR Signaling, Activation of Innate immune Response|
Application DetailsProduct Details anti-MAP2K6 Antibody Target Details MAP2K6 Handling Images back to top
WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat, The detection limit for MAP2K6 is approximately 0.5 ng/lane under reducing conditions.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested. Optimal dilutions should be determined by end users.
Antibody can be supported by chemiluminescence kit ABIN921124 in WB.
|Restrictions||For Research Use only|
HandlingProduct Details anti-MAP2K6 Antibody Target Details MAP2K6 Application Details Images back to top
|Reconstitution||Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.|
|Buffer||Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.|
|Preservative||Thimerosal (Merthiolate), Sodium azide|
|Precaution of Use||This product contains Thimerosal (Merthiolate),Sodium azide a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||-20 °C,4 °C|
At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
|Expiry Date||12 months|
ImagesProduct Details anti-MAP2K6 Antibody Target Details MAP2K6 Application Details Handling back to top