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anti-Dog (Canine) MAP2K7 antibody for Western Blotting

Recommended MAP2K7 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase Kinase 7 (MAP2K7) Antibodies
  • 2
  • 5930412N11Rik
  • JNKK
  • JNKK 2
  • Jnkk2
  • MAP2K7
  • map2k7-A
  • MAPKK 7
  • MAPKK7
  • Mapkk7
  • MEK 7
  • Mek7
  • MKK7
  • Mkk7
  • Prkmk7
  • PRKMK7
  • SAPKK-4
  • SAPKK4
  • sek2
  • si:ch211-254d18.2
Reactivity
Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
125
60
50
1
1
Host
Mouse
112
21
Clonality
Monoclonal
Conjugate
This MAP2K7 antibody is un-conjugated
5
5
3
2
2
2
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Western Blotting (WB)
113
69
45
15
14
11
9
6
5
2
1
1
1
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Catalog No. ABIN126842
$ 533.50
Plus shipping costs $45.00

Similar anti-MAP2K7 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 21 Antibodies 25 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 1 Antibodies
Flow Cytometry (FACS) 1 Antibodies 1 Antibodies
Immunocytochemistry (ICC) 4 Antibodies 5 Antibodies
Immunofluorescence (IF) 4 Antibodies 4 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies 11 Antibodies
Immunohistochemistry (IHC) 18 Antibodies 20 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 5 Antibodies 8 Antibodies
Immunoprecipitation (IP) 4 Antibodies
Western Blotting (WB) 42 Antibodies

General

Antigen Mitogen-Activated Protein Kinase Kinase 7 (MAP2K7) Antibodies
Reactivity Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
(125), (60), (50), (1), (1)
Host Mouse
(112), (21)
Clonality (Clone)
Monoclonal   ( )
Conjugate This MAP2K7 antibody is un-conjugated
(5), (5), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Western Blotting (WB)
(113), (69), (45), (15), (14), (11), (9), (6), (5), (2), (1), (1), (1)
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Product Details anti-MAP2K7 Antibody

Target Details MAP2K7 Application Details Handling Images
Specificity This antibody specifically recognizes the N-terminus of MKK7.
Characteristics Synonyms: MAPK/ERK kinase 7, JNK-activating kinase 2, c-Jun N-terminal kinase kinase 2, MAPKK 7,NKK2, MEK7, MKK7, MAP kinase kinase 7, Dual specificity mitogen-activated protein kinasekinase 7
Purification Size exclusion chromatography
Components incl. positive Control
Immunogen Synthetic peptide conjugated to KLH
Clone 10F7
Isotype IgG1

Target Details MAP2K7

Product Details anti-MAP2K7 Antibody Application Details Handling Images back to top
Antigen
Alternative Name MAP2K7 (MAP2K7 Antibody Abstract)
Background The stress response of mammalian cells results in activation of kinases of the SAPK1 and SAPK2 families. The SAP kinases are specifically activated by dual phosphorylation of a conserved T-X-Y motif by MAP kinase kinases (MKK). The 3 isoforms of the SAPK1 family (SAPK1α/jnk2, SAPK1β/jnk3, SAPK1γ/jnk1) are synergistically activated by phosphorylation of the threonine residue by MKK7 and the tyrosine residue by MKK4.Synonyms: Dual specificity mitogen-activated protein kinase kinase 7, JNK-activating kinase 2, MAP kinase kinase 7, MAPK/ERK kinase 7, MAPKK 7, MEK7, MKK7, NKK2, c-Jun N-terminal kinase kinase 2
Molecular Weight 45 kDa
Gene ID 5609
UniProt O14733
Research Area Kinases/Phosphatases
Pathways MAPK Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway

Application Details

Product Details anti-MAP2K7 Antibody Target Details MAP2K7 Handling Images back to top
Application Notes Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. Positive Control: Cell lysate from untreated SW480 cells. ELISA: 0.1 μg/mL. Immunocytochemistry. Immunohistochemistry on frozen sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-MAP2K7 Antibody Target Details MAP2K7 Application Details Images back to top
Reconstitution Restore with 1 mL H2O (15 min, RT).
Buffer 1 mL2 x PBS containing 0.09 % Sodium Azide, PEG and Sucrose
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C/-80 °C
Storage Comment Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch.
Expiry Date 12 months

Images

Product Details anti-MAP2K7 Antibody Target Details MAP2K7 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-MAP2K7 antibody (Mitogen-Activated Protein Kinase Kinase 7) (ABIN126842) anti-Mitogen-Activated Protein Kinase Kinase 7 (MAP2K7) antibody