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anti-Human MAP3K1 antibody for Enzyme Immunoassay

Recommended MAP3K1 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase Kinase Kinase 1 (MAP3K1) Antibodies
  • ARAKIN
  • ATMEKK1
  • MAP3K1
  • MAPK/ERK kinase kinase 1
  • MAPKKK1
  • MAPKKK8
  • MEKK
  • Mekk
  • Mekk1
  • MEKK1
  • MEKK 1
  • SRXY6
  • T15F16.5
  • T15F16_5
Epitope
C-Term
44
20
12
10
6
6
3
3
2
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Human
114
49
26
6
6
4
1
1
Host
Rabbit
107
31
Clonality
Polyclonal
Conjugate
This MAP3K1 antibody is un-conjugated
7
7
7
7
6
6
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
98
60
38
30
25
18
13
3
2
2
1
1
1
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Catalog No. ABIN360286
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008787446 ABIN358289 EIA Rabbit Ig pThr1383 Log in to see Polyclonal 2

Top referenced anti-MAP3K1 antibody for Enzyme Immunoassay

Similar anti-MAP3K1 Antibodies

Application / Reactivity Human
Dot Blot (DB) 18 Antibodies
ELISA 45 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Flow Cytometry (FACS) 25 Antibodies
Immunocytochemistry (ICC) 3 Antibodies
Immunofluorescence (IF) 13 Antibodies
Immunohistochemistry (IHC) 18 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 36 Antibodies
Immunoprecipitation (IP) 2 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Western Blotting (WB) 80 Antibodies

General

Antigen Mitogen-Activated Protein Kinase Kinase Kinase 1 (MAP3K1) Antibodies
Epitope C-Term
(44), (20), (12), (10), (6), (6), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(114), (49), (26), (6), (6), (4), (1), (1)
Host Rabbit
(107), (31)
Clonality
Polyclonal
Conjugate This MAP3K1 antibody is un-conjugated
(7), (7), (7), (7), (6), (6), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(98), (60), (38), (30), (25), (18), (13), (3), (2), (2), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-MAP3K1 Antibody

Target Details MAP3K1 Application Details Handling References for anti-MAP3K1 Antibody (ABIN360286) Images
Specificity This antibody reacts to MEKK1.
Purification Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human MEKK1.
Isotype Ig

Target Details MAP3K1

Product Details anti-MAP3K1 Antibody Application Details Handling References for anti-MAP3K1 Antibody (ABIN360286) Images back to top
Antigen
Alternative Name MAP3K1 (MAP3K1 Antibody Abstract)
Background Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MEKK1 can phosphorylate and activate MAPKK 1 and MAPKK 2 (MEK1/MEK2) which leads to phosphorylation of MAP kinases. It is also a highly efficient activator of the JNK cascade. The protein contains a putative 1 RING-type zinc finger and 1 SWIM-type zinc finger.Synonyms: MAPK/ERK kinase kinase 1, MAPKKK1, MEKK, MEKK1, Mitogen-activated protein kinase kinase kinase 1
Gene ID 4214, 9606
UniProt Q13233
Research Area Kinases/Phosphatases
Pathways MAPK Signaling, Interferon-gamma Pathway, Caspase Cascade in Apoptosis, TLR Signaling, Fc-epsilon Receptor Signaling Pathway

Application Details

Product Details anti-MAP3K1 Antibody Target Details MAP3K1 Handling References for anti-MAP3K1 Antibody (ABIN360286) Images back to top
Application Notes ELISA: 1/1,000. Western blotting: 1/100 - 1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-MAP3K1 Antibody Target Details MAP3K1 Application Details References for anti-MAP3K1 Antibody (ABIN360286) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-MAP3K1 Antibody (ABIN360286)

Product Details anti-MAP3K1 Antibody Target Details MAP3K1 Application Details Handling Images back to top
Background publications

Xia, Wu, Su, Murray, Karin: "JNKK1 organizes a MAP kinase module through specific and sequential interactions with upstream and downstream components mediated by its amino-terminal extension." in: Genes & development, Vol. 12, Issue 21, pp. 3369-81, 1998

Vinik, Kay, Fiedorek: "Mapping of the MEK kinase gene (Mekk) to mouse chromosome 13 and human chromosome 5." in: Mammalian genome : official journal of the International Mammalian Genome Society, Vol. 6, Issue 11, pp. 782-3, 1996

Images

Product Details anti-MAP3K1 Antibody Target Details MAP3K1 Application Details Handling References for anti-MAP3K1 Antibody (ABIN360286) back to top
Supplier Images
 image for anti-MAP3K1 antibody (Mitogen-Activated Protein Kinase Kinase Kinase 1) (C-Term) (ABIN360286) anti-Mitogen-Activated Protein Kinase Kinase Kinase 1 (MAP3K1) (C-Term) antibody