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anti-Human MAP3K1 antibody for Enzyme Immunoassay

Recommended MAP3K1 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase Kinase Kinase 1 (MAP3K1) Antibodies
  • ARAKIN
  • ATMEKK1
  • MAP3K1
  • MAPK/ERK kinase kinase 1
  • MAPKKK1
  • MAPKKK8
  • MEKK
  • Mekk
  • Mekk1
  • MEKK1
  • MEKK 1
  • SRXY6
  • T15F16.5
  • T15F16_5
Epitope
pThr1383
45
19
12
10
6
6
3
3
2
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Human
114
49
26
6
6
4
1
1
Host
Rabbit
107
31
Clonality
Polyclonal
Conjugate
This MAP3K1 antibody is un-conjugated
7
7
7
7
6
6
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA)
99
60
38
30
25
18
13
3
2
2
1
1
1
Supplier
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Catalog No. ABIN358289
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008858215 ABIN360286 EIA WB Rabbit Ig C-Term Log in to see Polyclonal 2

Top referenced anti-MAP3K1 antibody for Enzyme Immunoassay

Similar anti-MAP3K1 Antibodies

Application / Reactivity Human
Dot Blot (DB) 18 Antibodies
ELISA 45 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Flow Cytometry (FACS) 25 Antibodies
Immunocytochemistry (ICC) 3 Antibodies
Immunofluorescence (IF) 13 Antibodies
Immunohistochemistry (IHC) 19 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 37 Antibodies
Immunoprecipitation (IP) 2 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Western Blotting (WB) 81 Antibodies

General

Antigen Mitogen-Activated Protein Kinase Kinase Kinase 1 (MAP3K1) Antibodies
Epitope pThr1383
(45), (19), (12), (10), (6), (6), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(114), (49), (26), (6), (6), (4), (1), (1)
Host Rabbit
(107), (31)
Clonality
Polyclonal
Conjugate This MAP3K1 antibody is un-conjugated
(7), (7), (7), (7), (6), (6), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA)
(99), (60), (38), (30), (25), (18), (13), (3), (2), (2), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-MAP3K1 Antibody

Target Details MAP3K1 Application Details Handling References for anti-MAP3K1 Antibody (ABIN358289) Images
Specificity This antibody detects MAP3K1 pThr1383. Predicted to cross react with Mouse (100 % Antigen Homology).
Purification Protein A Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with peptides. The antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T1383 of human MAP3K1.
Isotype Ig

Target Details MAP3K1

Product Details anti-MAP3K1 Antibody Application Details Handling References for anti-MAP3K1 Antibody (ABIN358289) Images back to top
Antigen
Alternative Name MAP3K1 (MAP3K1 Antibody Abstract)
Background Component of a protein kinase signal transduction cascade. Activates the ERK and JNK kinase pathways by phosphorylation of MAP2K1 and MAP2K4. Activates CHUK and IKBKB, the central protein kinases of the NF-kappa-B pathway.Synonyms: MAPK/ERK kinase kinase 1, MAPKKK1, MEKK, MEKK1, Mitogen-activated protein kinase kinase kinase 1
Molecular Weight 164340 Da
Gene ID 4214, 9606
UniProt Q13233
Research Area Kinases/Phosphatases
Pathways MAPK Signaling, Interferon-gamma Pathway, Caspase Cascade in Apoptosis, TLR Signaling, Fc-epsilon Receptor Signaling Pathway

Application Details

Product Details anti-MAP3K1 Antibody Target Details MAP3K1 Handling References for anti-MAP3K1 Antibody (ABIN358289) Images back to top
Application Notes ELISA: 1/1,000. Dot Blot: 1/100-1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-MAP3K1 Antibody Target Details MAP3K1 Application Details References for anti-MAP3K1 Antibody (ABIN358289) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-MAP3K1 Antibody (ABIN358289)

Product Details anti-MAP3K1 Antibody Target Details MAP3K1 Application Details Handling Images back to top
Background publications

Mokhtari, Myers, Welsh: "MAPK kinase kinase-1 is essential for cytokine-induced c-Jun NH2-terminal kinase and nuclear factor-kappaB activation in human pancreatic islet cells." in: Diabetes, Vol. 57, Issue 7, pp. 1896-904, 2008

Dassé, Bridoux, Baranek, Lambert, Salesse, Sowa, Martiny, Trentesaux, Petitfrère: "Tissue inhibitor of metalloproteinase-1 promotes hematopoietic differentiation via caspase-3 upstream the MEKK1/MEK6/p38alpha pathway." in: Leukemia, Vol. 21, Issue 4, pp. 595-603, 2007

Images

Product Details anti-MAP3K1 Antibody Target Details MAP3K1 Application Details Handling References for anti-MAP3K1 Antibody (ABIN358289) back to top
Supplier Images
 image for anti-MAP3K1 antibody (Mitogen-Activated Protein Kinase Kinase Kinase 1) (pThr1383) (ABIN358289) anti-Mitogen-Activated Protein Kinase Kinase Kinase 1 (MAP3K1) (pThr1383) antibody