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|Antigen||Mitogen-Activated Protein Kinase Kinase Kinase 1 (MAP3K1) Antibodies|
|Conjugate||This MAP3K1 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Western Blotting (WB)
|2 references available|
|Supplier||Log in to see|
Product Details anti-MAP3K1 AntibodyTarget Details MAP3K1 Application Details Handling References for anti-MAP3K1 antibody (ABIN360286) Images
|Specificity||This antibody reacts to MEKK1.|
|Purification||Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS|
|Immunogen||This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human MEKK1.|
Target Details MAP3K1Product Details anti-MAP3K1 Antibody Application Details Handling References for anti-MAP3K1 antibody (ABIN360286) Images back to top
|Alternative Name||MAP3K1 (MAP3K1 Antibody Abstract)|
|Background||Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MEKK1 can phosphorylate and activate MAPKK 1 and MAPKK 2 (MEK1/MEK2) which leads to phosphorylation of MAP kinases. It is also a highly efficient activator of the JNK cascade. The protein contains a putative 1 RING-type zinc finger and 1 SWIM-type zinc finger.Synonyms: MAPK/ERK kinase kinase 1, MAPKKK1, MEKK, MEKK1, Mitogen-activated protein kinase kinase kinase 1|
|Gene ID||4214, 9606|
|Pathways||MAPK Signaling, Interferon-gamma Pathway, Caspase Cascade in Apoptosis, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Regulation of Actin Filament Polymerization, Toll-Like Receptors Cascades|
Application DetailsProduct Details anti-MAP3K1 Antibody Target Details MAP3K1 Handling References for anti-MAP3K1 antibody (ABIN360286) Images back to top
ELISA: 1/1,000. Western blotting: 1/100 - 1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-MAP3K1 Antibody Target Details MAP3K1 Application Details References for anti-MAP3K1 antibody (ABIN360286) Images back to top
|Buffer||PBS with 0.09 % (W/V) sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.|
References for anti-MAP3K1 antibody (ABIN360286)Product Details anti-MAP3K1 Antibody Target Details MAP3K1 Application Details Handling Images back to top
Xia, Wu, Su, Murray, Karin: "JNKK1 organizes a MAP kinase module through specific and sequential interactions with upstream and downstream components mediated by its amino-terminal extension." in: Genes & development, Vol. 12, Issue 21, pp. 3369-81, 1998
Vinik, Kay, Fiedorek: "Mapping of the MEK kinase gene (Mekk) to mouse chromosome 13 and human chromosome 5." in: Mammalian genome : official journal of the International Mammalian Genome Society, Vol. 6, Issue 11, pp. 782-3, 1996
ImagesProduct Details anti-MAP3K1 Antibody Target Details MAP3K1 Application Details Handling References for anti-MAP3K1 antibody (ABIN360286) back to top