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|Antigen||Mitogen-Activated Protein Kinase Kinase Kinase 1 (MAP3K1) Antibodies|
|Epitope||AA 1418-1432, C-Term Alternatives|
|Conjugate||This MAP3K1 antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-MAP3K1 AntibodyTarget Details MAP3K1 Application Details Handling Images
|Purpose||Rabbit IgG polyclonal antibody for Mitogen-activated protein kinase kinase kinase 1(MAP3K1) detection. Tested with WB, IHC-P in Human,Mouse,Rat.|
Predicted Cross Reactivity: mouse, rat
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.
Rabbit IgG polyclonal antibody for Mitogen-activated protein kinase kinase kinase 1(MAP3K1) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase
Protein Name: Mitogen-activated protein kinase kinase kinase 1
|Purification||Immunogen affinity purified.|
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human MEKK1(1418-1432aa PEVLRGQQYGRSCDV), identical to the related rat and mouse sequences.|
Target Details MAP3K1Product Details anti-MAP3K1 Antibody Application Details Handling Images back to top
|Alternative Name||MAP3K1 (MAP3K1 Antibody Abstract)|
MAP3K1(Mitogen-activated protein kinase kinase kinase 1), also known as MEKK1, MAPKKK1, MEK KINASE or MAP/ERK KINASE KINASE 1, is an enzyme that in humans is encoded by the MAP3K1 gene. Vinik et al.(1995) identified DNA sequence and size polymorphisms in intronic and 3-prime untranslated regions of the mouse Map3k1 gene and the human MAP3K1 homolog. Using these allele-specific polymorphisms, they mapped the Map3k1 gene in an intersubspecific backcross to mouse chromosome 13. They mapped the human MAP3K1 gene to chromosome 5 by somatic cell hybrid analysis. By assaying transfected COS-1 cells, Xia et al.(1998) showed that human MEKK1 activated JNK1(MAPK8) robustly and p38-alpha(MAPK14) less efficiently, but it had only a marginal effect on ERK2(MAPK1). MEKK1 directly and specifically interacted with JNKK1(MAP2K4) and activated JNKK1 in cells and in vitro. Phosphorylation of JNKK1 by MEKK1 disrupted their interaction. MEKK1 and JNK1 competed for binding to JNKK1. Xia et al.(1998) concluded that JNKK1 is the preferred MEKK1 substrate.
Synonyms: M3K1_HUMAN antibody|MAP3K1 antibody|MAPK/ERK kinase kinase 1 antibody|MAPKKK1 antibody|MEK kinase 1 antibody|MEKK 1 antibodyMekk antibody|MEKK1 antibody|Mitogen activated protein kinase kinase kinase 1 antibody|Mitogen activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase antibody|Mitogen-activated protein kinase kinase kinase 1 antibodySRXY6 antibody
|Pathways||MAPK Signaling, Interferon-gamma Pathway, Caspase Cascade in Apoptosis, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Regulation of Actin Filament Polymerization, Toll-Like Receptors Cascades|
Application DetailsProduct Details anti-MAP3K1 Antibody Target Details MAP3K1 Handling Images back to top
WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat, The detection limit for MAP3K1 is approximately 0.5 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
|Restrictions||For Research Use only|
HandlingProduct Details anti-MAP3K1 Antibody Target Details MAP3K1 Application Details Images back to top
|Reconstitution||Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.|
|Buffer||Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.|
|Preservative||Thimerosal (Merthiolate), Sodium azide|
|Precaution of Use||This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
|Expiry Date||12 months|
ImagesProduct Details anti-MAP3K1 Antibody Target Details MAP3K1 Application Details Handling back to top