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anti-Human MAP3K1 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended MAP3K1 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase Kinase Kinase 1 (MAP3K1) Antibodies
  • MAP3K1
  • Mekk1
  • MAPKKK1
  • MEKK1
  • Mekk
  • ARAKIN
  • ATMEKK1
  • MAPK/ERK kinase kinase 1
  • MAPKKK8
  • T15F16.5
  • T15F16_5
  • MEKK
  • MEKK 1
  • SRXY6
  • mitogen-activated protein kinase kinase kinase 1
  • similar to MEK kinase 1
  • mitogen activated protein kinase kinase kinase 1
  • mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase
  • MAP3K1
  • map3k1
  • Map3k1
  • MEKK1
Epitope
AA 1418-1432, C-Term
37
18
11
9
7
3
3
3
3
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
109
53
31
10
9
7
4
4
3
3
3
3
2
1
1
Host
Rabbit
100
31
Clonality
Polyclonal
Conjugate
This MAP3K1 antibody is un-conjugated
7
7
7
7
6
6
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
94
60
37
28
25
17
13
3
2
2
2
2
2
1
Options
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Catalog No. ABIN3044007
$ 200.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN392403 IHC (p) WB Rabbit Ig AA 1145-1176, C-Term Log in to see Polyclonal
1 ABIN3025243 FACS IF IHC (p) WB Mouse IgG2a, kappa AA 1211-1310 Log in to see 2F6
1 ABIN2625096 IHC (p) WB Rabbit IgG AA 1418-1432 Log in to see Polyclonal
1 ABIN2884017 ELISA IHC (p) WB Rabbit IgG pThr1402 Log in to see Polyclonal
1 ABIN4333513 FACS IF IHC (p) Mouse IgG2a kappa Log in to see 2F6
1 ABIN4333534 FACS IHC (p) WB FITC Mouse IgG2a kappa Log in to see 2F6
1 ABIN4333518 FACS IHC (p) WB DyLight 405 Mouse IgG2a kappa Log in to see 2F6
1 ABIN4333523 FACS IHC (p) WB DyLight 680 Mouse IgG2a kappa Log in to see 2F6
1 ABIN4333521 FACS IHC (p) WB DyLight 550 Mouse IgG2a kappa Log in to see 2F6
1 ABIN4333529 FACS IHC (p) WB PE Mouse IgG2a kappa Log in to see 2F6
1 ABIN4333512 FACS IF IHC (p) Mouse IgG2a kappa Log in to see 2F6
1 ABIN4333517 FACS IHC (p) WB DyLight 350 Mouse IgG2a kappa Log in to see 2F6
1 ABIN4333522 FACS IHC (p) WB DyLight 650 Mouse IgG2a kappa Log in to see 2F6
1 ABIN4333524 FACS IHC (p) WB DyLight 755 Mouse IgG2a kappa Log in to see 2F6
1 ABIN4333525 FACS IHC (p) WB Alexa Fluor 405 Mouse IgG2a kappa Log in to see 2F6
1 ABIN4333526 FACS IHC (p) WB Alexa Fluor 488 Mouse IgG2a kappa Log in to see 2F6
1 ABIN4333528 FACS IHC (p) WB Alexa Fluor 700 Mouse IgG2a kappa Log in to see 2F6
1 ABIN4333530 FACS IHC (p) WB Allophycocyanin Mouse IgG2a kappa Log in to see 2F6
1 ABIN4333527 FACS IHC (p) WB Alexa Fluor 647 Mouse IgG2a kappa Log in to see 2F6
1 ABIN4333531 FACS IHC (p) WB PerCP Mouse IgG2a kappa Log in to see 2F6

Similar anti-MAP3K1 Antibodies

Application / Reactivity Human
Cytometry by Time of Flight (CyTOF) 2 Antibodies
Dot Blot (DB) 17 Antibodies
ELISA 45 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Flow Cytometry (FACS) 25 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 2 Antibodies
Immunofluorescence (IF) 13 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 2 Antibodies
Immunohistochemistry (IHC) 16 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 36 Antibodies
Immunoprecipitation (IP) 3 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Western Blotting (WB) 77 Antibodies

General

Antigen Mitogen-Activated Protein Kinase Kinase Kinase 1 (MAP3K1) Antibodies
Epitope AA 1418-1432, C-Term
(37), (18), (11), (9), (7), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(109), (53), (31), (10), (9), (7), (4), (4), (3), (3), (3), (3), (2), (1), (1)
Host Rabbit
(100), (31)
Clonality
Polyclonal
Conjugate This MAP3K1 antibody is un-conjugated
(7), (7), (7), (7), (6), (6), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(94), (60), (37), (28), (25), (17), (13), (3), (2), (2), (2), (2), (2), (1)
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Product Details anti-MAP3K1 Antibody

Target Details MAP3K1 Application Details Handling Images
Purpose Rabbit IgG polyclonal antibody for Mitogen-activated protein kinase kinase kinase 1(MAP3K1) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Sequence PEVLRGQQYG RSCDV
Cross-Reactivity (Details) Predicted Cross Reactivity: mouse, rat
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.
Characteristics Rabbit IgG polyclonal antibody for Mitogen-activated protein kinase kinase kinase 1(MAP3K1) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase
Protein Name: Mitogen-activated protein kinase kinase kinase 1
Purification Immunogen affinity purified.
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human MEKK1(1418-1432aa PEVLRGQQYGRSCDV), identical to the related rat and mouse sequences.
Isotype IgG

Target Details MAP3K1

Product Details anti-MAP3K1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name MAP3K1 (MAP3K1 Antibody Abstract)
Background MAP3K1(Mitogen-activated protein kinase kinase kinase 1), also known as MEKK1, MAPKKK1, MEK KINASE or MAP/ERK KINASE KINASE 1, is an enzyme that in humans is encoded by the MAP3K1 gene. Vinik et al.(1995) identified DNA sequence and size polymorphisms in intronic and 3-prime untranslated regions of the mouse Map3k1 gene and the human MAP3K1 homolog. Using these allele-specific polymorphisms, they mapped the Map3k1 gene in an intersubspecific backcross to mouse chromosome 13. They mapped the human MAP3K1 gene to chromosome 5 by somatic cell hybrid analysis. By assaying transfected COS-1 cells, Xia et al.(1998) showed that human MEKK1 activated JNK1(MAPK8) robustly and p38-alpha(MAPK14) less efficiently, but it had only a marginal effect on ERK2(MAPK1). MEKK1 directly and specifically interacted with JNKK1(MAP2K4) and activated JNKK1 in cells and in vitro. Phosphorylation of JNKK1 by MEKK1 disrupted their interaction. MEKK1 and JNK1 competed for binding to JNKK1. Xia et al.(1998) concluded that JNKK1 is the preferred MEKK1 substrate.

Synonyms: M3K1_HUMAN antibody|MAP3K1 antibody|MAPK/ERK kinase kinase 1 antibody|MAPKKK1 antibody|MEK kinase 1 antibody|MEKK 1 antibodyMekk antibody|MEKK1 antibody|Mitogen activated protein kinase kinase kinase 1 antibody|Mitogen activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase antibody|Mitogen-activated protein kinase kinase kinase 1 antibodySRXY6 antibody
UniProt Q13233
Research Area Kinases/Phosphatases
Pathways MAPK Signaling, Interferon-gamma Pathway, Caspase Cascade in Apoptosis, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Regulation of Actin Filament Polymerization, Toll-Like Receptors Cascades

Application Details

Product Details anti-MAP3K1 Antibody Target Details MAP3K1 Handling Images back to top
Application Notes WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat, The detection limit for MAP3K1 is approximately 0.5 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
Comment

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions For Research Use only

Handling

Product Details anti-MAP3K1 Antibody Target Details MAP3K1 Application Details Images back to top
Format Lyophilized
Reconstitution Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
Concentration 500 μg/mL
Buffer Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
Preservative Thimerosal (Merthiolate), Sodium azide
Precaution of Use This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
Expiry Date 12 months

Images

Product Details anti-MAP3K1 Antibody Target Details MAP3K1 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-MAP3K1 antibody (Mitogen-Activated Protein Kinase Kinase Kinase 1) (AA 1418-1432) (ABIN3044007) Anti-MEKK1 antibody, Western blotting All lanes: Anti MEKK1 at 0.5ug/ml WB: MCF-7 Wh...
Immunohistochemistry (IHC) image for anti-MAP3K1 antibody (Mitogen-Activated Protein Kinase Kinase Kinase 1) (AA 1418-1432) (ABIN3044007) Anti-MEKK1 antibody, IHC(P) IHC(P): Human Intestinal Cancer Tissue