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anti-Rat (Rattus) MAP3K1 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended MAP3K1 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase Kinase Kinase 1 (MAP3K1) Antibodies
  • MAP3K1
  • Mekk1
  • MAPKKK1
  • MEKK1
  • Mekk
  • ARAKIN
  • ATMEKK1
  • MAPK/ERK kinase kinase 1
  • MAPKKK8
  • T15F16.5
  • T15F16_5
  • MEKK
  • MEKK 1
  • SRXY6
  • mitogen-activated protein kinase kinase kinase 1
  • similar to MEK kinase 1
  • mitogen activated protein kinase kinase kinase 1
  • mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase
  • MAP3K1
  • map3k1
  • Map3k1
  • MEKK1
Epitope
C-Term
44
20
12
10
6
6
3
3
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
113
48
25
6
6
4
1
1
Host
Rabbit
105
31
Clonality
Polyclonal
Conjugate
This MAP3K1 antibody is un-conjugated
7
7
7
7
6
6
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
96
60
35
31
25
18
13
3
3
2
2
2
1
Supplier
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Catalog No. ABIN3031846
$ 391.88
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1736716 IHC (p) WB Rabbit IgG Log in to see Polyclonal

Similar anti-MAP3K1 Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus)
Cytometry by Time of Flight (CyTOF) 2 Antibodies
Western Blotting (WB) 79 Antibodies 40 Antibodies 22 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Immunoprecipitation (IP) 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 34 Antibodies 4 Antibodies 2 Antibodies
Immunohistochemistry (IHC) 19 Antibodies 23 Antibodies 12 Antibodies
Immunofluorescence (IF) 13 Antibodies 5 Antibodies 4 Antibodies
Immunocytochemistry (ICC) 3 Antibodies 1 Antibodies
Immunochromatography (IC) 1 Antibodies 1 Antibodies

General

Antigen Mitogen-Activated Protein Kinase Kinase Kinase 1 (MAP3K1) Antibodies
Epitope C-Term
(44), (20), (12), (10), (6), (6), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(113), (48), (25), (6), (6), (4), (1), (1)
Host Rabbit
(105), (31)
Clonality
Polyclonal
Conjugate This MAP3K1 antibody is un-conjugated
(7), (7), (7), (7), (6), (6), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(96), (60), (35), (31), (25), (18), (13), (3), (3), (2), (2), (2), (1)
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Product Details anti-MAP3K1 Antibody

Target Details MAP3K1 Application Details Handling Images
Purification Antigen affinity
Immunogen An amino acid sequence from the C-terminus of human MEK kinase 1 (PEVLRGQQYGRSCDV) was used as the immunogen for this MAP3K1 antibody (100% homologous in human, mouse and rat).
Isotype IgG

Target Details MAP3K1

Product Details anti-MAP3K1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name MAP3K1 (MEKK1) (MAP3K1 Antibody Abstract)
Background Mitogen-activated protein kinase kinase kinase 1, also known as MEKK1, MAPKKK1, MEK Kinase or MAP/ERK Kinase Kinase 1, is an enzyme that in humans is encoded by the MAP3K1 gene. Vinik et al.(1995) identified DNA sequence and size polymorphisms in intronic and 3-prime untranslated regions of the mouse gene and the human homolog. Using these allele-specific polymorphisms, they mapped the mouse gene in an intersubspecific backcross to chromosome 13. They mapped the human gene to chromosome 5 by somatic cell hybrid analysis. By assaying transfected COS-1 cells, Xia et al.(1998) showed that human MAP3K1 / MEKK1 activated JNK1 (MAPK8) robustly and p38-alpha (MAPK14) less efficiently, but it had only a marginal effect on ERK2 (MAPK1). MAP3K1 / MEKK1 directly and specifically interacted with JNKK1 (MAP2K4) and activated JNKK1 in cells and in vitro. Phosphorylation of JNKK1 disrupted their interaction. MEKK1 and JNK1 competed for binding to JNKK1. Xia et al.(1998) concluded that JNKK1 is the preferred MEKK1 substrate.
UniProt Q13233
Pathways MAPK Signaling, Interferon-gamma Pathway, Caspase Cascade in Apoptosis, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response

Application Details

Product Details anti-MAP3K1 Antibody Target Details MAP3K1 Handling Images back to top
Application Notes The stated application concentrations are suggested starting amounts. Titration of the MAP3K1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL
Restrictions For Research Use only

Handling

Product Details anti-MAP3K1 Antibody Target Details MAP3K1 Application Details Images back to top
Buffer 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water
Storage -20 °C
Storage Comment After reconstitution, the MAP3K1 antibody can be stored for up to one month at 4 °C. For long-term, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Images

Product Details anti-MAP3K1 Antibody Target Details MAP3K1 Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-MAP3K1 antibody (Mitogen-Activated Protein Kinase Kinase Kinase 1) (C-Term) (ABIN3031846) IHC-P: MAP3K1 antibody testing of human intestine cancer
Western Blotting (WB) image for anti-MAP3K1 antibody (Mitogen-Activated Protein Kinase Kinase Kinase 1) (C-Term) (ABIN3031846) Western blot testing with MAP3K1 antibody and MCF-7 lysate. Predicted/observed size ...