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anti-Mouse (Murine) MAP3K12 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended MAP3K12 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase Kinase Kinase 12 (MAP3K12) Antibodies
  • Delta1
  • DKFZp459K2433
  • DLK
  • dlk
  • DLK-1
  • FA1
  • map3k12
  • map3k12a
  • mekk12
  • MEKK12
  • MGC151798
  • muk
  • MUK
  • pG2
  • PK
  • Pref-1
  • PREF1
  • zgc:77370
  • ZOG
  • ZPK
  • zpk
  • Zpk
  • ZPKP1
  • zpkp1
Epitope
C-Term
19
6
4
2
2
1
1
Reactivity
Human, Mouse (Murine)
47
13
8
Host
Rabbit
37
13
Clonality
Polyclonal
Conjugate
This MAP3K12 antibody is un-conjugated
5
3
3
3
3
3
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
44
29
27
3
2
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Catalog No. ABIN359408
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00089245656 ABIN391319 IHC (p) WB Rabbit Ig AA 828-859, C-Term Log in to see Polyclonal 7

Top referenced anti-MAP3K12 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Similar anti-MAP3K12 Antibodies

Application / Reactivity Mouse (Murine) Human
ELISA 4 Antibodies 29 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 1 Antibodies
Immunocytochemistry (ICC) 2 Antibodies
Immunohistochemistry (IHC) 5 Antibodies 25 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 2 Antibodies 4 Antibodies
Western Blotting (WB) 10 Antibodies 42 Antibodies

General

Antigen Mitogen-Activated Protein Kinase Kinase Kinase 12 (MAP3K12) Antibodies
Epitope C-Term
(19), (6), (4), (2), (2), (1), (1)
Reactivity Human, Mouse (Murine)
(47), (13), (8)
Host Rabbit
(37), (13)
Clonality
Polyclonal
Conjugate This MAP3K12 antibody is un-conjugated
(5), (3), (3), (3), (3), (3)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(44), (29), (27), (3), (2)
Pubmed 5 references available
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Product Details anti-MAP3K12 Antibody

Target Details MAP3K12 Application Details Handling References for anti-MAP3K12 Antibody (ABIN359408) Images
Specificity This antibody detects MAP3K12 at C-term.
Purification Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human ZPK.
Isotype Ig

Target Details MAP3K12

Product Details anti-MAP3K12 Antibody Application Details Handling References for anti-MAP3K12 Antibody (ABIN359408) Images back to top
Antigen
Alternative Name MAP3K12 (MAP3K12 Antibody Abstract)
Background ZPK is a member of serine/threonine protein kinase family. This kinase contains a leucine-zipper domain, and is predominately expressed in neuronal cells. The phosphorylation state of this kinase in synaptic terminals was shown to be regulated by membrane depolarization via calcineurin. This kinase forms heterodimers with leucine zipper containing transcription factors, such as cAMP responsive element binding protein (CREB) and MYC, and thus may play a regulatory role in PKA or retinoic acid induced neuronal differentiation.Synonyms: Dual leucine zipper bearing kinase, Leucine-zipper protein kinase, MAPK-upstream kinase, Mitogen-activated protein kinase kinase kinase 12, Mixed lineage kinase
Molecular Weight 93188 Da
Gene ID 7786, 9606
UniProt Q12852
Research Area Signaling
Pathways MAPK Signaling

Application Details

Product Details anti-MAP3K12 Antibody Target Details MAP3K12 Handling References for anti-MAP3K12 Antibody (ABIN359408) Images back to top
Application Notes ELISA 1: 1,000. Western blot 1: 100 - 1: 500. Immunohistochemistry 1: 50 - 1: 100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-MAP3K12 Antibody Target Details MAP3K12 Application Details References for anti-MAP3K12 Antibody (ABIN359408) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.

References for anti-MAP3K12 Antibody (ABIN359408)

Product Details anti-MAP3K12 Antibody Target Details MAP3K12 Application Details Handling Images back to top
Background publications

Itoh, Wang, Ito, Reddy, Itoh: "SP3 acts as a positive regulator on the core promoter of human ZPK gene." in: Biochemical and biophysical research communications, Vol. 313, Issue 3, pp. 612-8, 2003

Fukuyama, Yoshida, Yamashita, Deyama, Baba, Suzuki, Mohri, Ikezawa, Nakajima, Hirai, Ohno: "MAPK upstream kinase (MUK)-binding inhibitory protein, a negative regulator of MUK/dual leucine zipper-bearing kinase/leucine zipper protein kinase." in: The Journal of biological chemistry, Vol. 275, Issue 28, pp. 21247-54, 2000

Reddy, Basu, Bannerman, Ikegaki, Reddy, Pleasure: "ZPK inhibits PKA induced transcriptional activation by CREB and blocks retinoic acid induced neuronal differentiation." in: Oncogene, Vol. 18, Issue 31, pp. 4474-84, 1999

Mata, Merritt, Fan, Yu, Holzman: "Characterization of dual leucine zipper-bearing kinase, a mixed lineage kinase present in synaptic terminals whose phosphorylation state is regulated by membrane depolarization via calcineurin." in: The Journal of biological chemistry, Vol. 271, Issue 28, pp. 16888-96, 1996

Curran: "It is better to travel hopefully ellipsis" in: Chemistry & biology, Vol. 3, Issue 8, pp. 641-2, 1996

Images

Product Details anti-MAP3K12 Antibody Target Details MAP3K12 Application Details Handling References for anti-MAP3K12 Antibody (ABIN359408) back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-MAP3K12 antibody (Mitogen-Activated Protein Kinase Kinase Kinase 12) (C-Term) (ABIN359408) Formalin-fixed and paraffin-embedded human cancer tissue (breast carcinoma) reacted w...
Western Blotting (WB) image for anti-MAP3K12 antibody (Mitogen-Activated Protein Kinase Kinase Kinase 12) (C-Term) (ABIN359408) Western blot analysis of anti-ZPK C-term Pab in HL-60 cell lysate (lane A) and in mou...