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|Antigen||Mitogen-Activated Protein Kinase Kinase Kinase 3 (MAP3K3) Antibodies|
|Epitope||AA 27-135 Alternatives|
|Reactivity||Dog (Canine), Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This MAP3K3 antibody is un-conjugated Alternatives|
Immunofluorescence (IF), Western Blotting (WB)
|2 references available|
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Product Details anti-MAP3K3 AntibodyTarget Details MAP3K3 Application Details Handling References for anti-MAP3K3 antibody (ABIN968365) Images
|Cross-Reactivity||Mouse (Murine), Rat (Rattus), Dog (Canine)|
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Please refer to us for technical protocols.
|Purification||The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.|
|Immunogen||Human MEKK3 aa. 27-135|
Target Details MAP3K3Product Details anti-MAP3K3 Antibody Application Details Handling References for anti-MAP3K3 antibody (ABIN968365) Images back to top
|Alternative Name||MEKK3 (MAP3K3 Antibody Abstract)|
|Background||The Ras signaling pathway links signals from growth factor receptors to activation of the MEK/ERK kinases. This cascade of phosphorylation leads to cell growth and differentiation. External stimuli such as endotoxins, UV irradiation, heat, and hyperosmolarity, induce an array of cellular responses that culminate with gene expression. These processes are controlled by the MKK3/p38MAP kinase cascade. A third pathway responsive to environmental stress, cell death, or apoptosis is regulated by SEK/SAPKs. When overexpresssed, MEKK3 is a kinase that is capable of activating both the ERK and SAPK cascades. MEKK3 is 626 amino acids long and is ubiquitously expressed. It is positioned upstream of SEK and MEK in the signaling pathways and directly phosphorylates these enzymes. Overexpression of MEKK3 results in the activation of NFkappaB. Thus, MEKK3 represents an emerging family of kinases that is capable of inducing more than one signaling pathway. This indicates that specificity in the signaling cascades is probably achieved by effector kinases such as the ERKs and SAPKs.|
|Molecular Weight||71 kDa|
|Research Area||Immunology, Innate Immunity, Kinases/Phosphatases|
Application DetailsProduct Details anti-MAP3K3 Antibody Target Details MAP3K3 Handling References for anti-MAP3K3 antibody (ABIN968365) Images back to top
Related Products: ABIN967389, ABIN968535
|Restrictions||For Research Use only|
HandlingProduct Details anti-MAP3K3 Antibody Target Details MAP3K3 Application Details References for anti-MAP3K3 antibody (ABIN968365) Images back to top
|Buffer||Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage Comment||Store undiluted at -20°C.|
References for anti-MAP3K3 antibody (ABIN968365)Product Details anti-MAP3K3 Antibody Target Details MAP3K3 Application Details Handling Images back to top
|Product cited in:||
Yang, Lai, Lai, Hsu, Kuo, Yu, Lin, Yang, Kuo, Wu, Chung: "Phenethyl isothiocyanate inhibits migration and invasion of human gastric cancer AGS cells through suppressing MAPK and NF-kappaB signal pathways." in: Anticancer research, Vol. 30, Issue 6, pp. 2135-43, 2010
Ellinger-Ziegelbauer, Brown, Kelly, Siebenlist et al.: "Direct activation of the stress-activated protein kinase (SAPK) and extracellular signal-regulated protein kinase (ERK) pathways by an inducible mitogen-activated protein Kinase/ERK kinase kinase 3 ..." in: The Journal of biological chemistry, Vol. 272, Issue 5, pp. 2668-74, 1997
ImagesProduct Details anti-MAP3K3 Antibody Target Details MAP3K3 Application Details Handling References for anti-MAP3K3 antibody (ABIN968365) back to top