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anti-Human MAP3K3 antibody for Immunofluorescence

Recommended MAP3K3 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase Kinase Kinase 3 (MAP3K3) Antibodies
  • AW548911
  • MAPKKK3
  • MEKK3
  • Mekk3
  • mKIAA4031
Epitope
AA 27-135
17
15
10
10
5
4
3
2
2
1
1
1
1
1
1
Reactivity
Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
39
34
7
2
2
2
1
1
1
1
1
1
1
Host
Mouse
51
8
Clonality
Monoclonal
Conjugate
This MAP3K3 antibody is un-conjugated
2
2
2
2
2
2
Application
Immunofluorescence (IF), Western Blotting (WB)
55
23
8
7
4
1
1
1
Supplier
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Catalog No. ABIN968365
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.3161046 ABIN968366 IF WB Mouse IgG1 AA 27-135 Log in to see 40-MEKK3 2
0.3161046 ABIN2706529 IC IF IHC WB Rabbit Center Log in to see Polyclonal
0.0010850558 ABIN2892177 IF IHC WB Rabbit Asn96 Log in to see Polyclonal
0.0010850558 ABIN3185519 ELISA IF IHC WB Rabbit IgG Internal Region Log in to see Polyclonal
0.0010850558 ABIN408256 ICC IF IHC (p) IHC WB Rabbit IgG Asp96 Log in to see Polyclonal

Top referenced anti-MAP3K3 antibody for Immunofluorescence

Similar anti-MAP3K3 Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus) Dog (Canine)
Western Blotting (WB) 33 Antibodies 38 Antibodies 7 Antibodies 3 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 3 Antibodies 8 Antibodies 2 Antibodies
Immunohistochemistry (IHC) 6 Antibodies 7 Antibodies 3 Antibodies 1 Antibodies
Immunofluorescence (IF) 6 Antibodies 5 Antibodies 3 Antibodies 2 Antibodies
Immunocytochemistry (ICC) 2 Antibodies 1 Antibodies
Immunochromatography (IC) 1 Antibodies

General

Antigen Mitogen-Activated Protein Kinase Kinase Kinase 3 (MAP3K3) Antibodies
Epitope AA 27-135
(17), (15), (10), (10), (5), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
(39), (34), (7), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Host Mouse
(51), (8)
Clonality (Clone)
Monoclonal   ( )
Conjugate This MAP3K3 antibody is un-conjugated
(2), (2), (2), (2), (2), (2)
Application Immunofluorescence (IF), Western Blotting (WB)
(55), (23), (8), (7), (4), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-MAP3K3 Antibody

Target Details MAP3K3 Application Details Handling References for anti-MAP3K3 Antibody (ABIN968365) Images
Cross-Reactivity Mouse (Murine), Rat (Rattus), Dog (Canine)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Please refer to us for technical protocols.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Human MEKK3 aa. 27-135
Clone 40-MEKK3
Isotype IgG1

Target Details MAP3K3

Product Details anti-MAP3K3 Antibody Application Details Handling References for anti-MAP3K3 Antibody (ABIN968365) Images back to top
Antigen
Alternative Name MEKK3 (MAP3K3 Antibody Abstract)
Background The Ras signaling pathway links signals from growth factor receptors to activation of the MEK/ERK kinases. This cascade of phosphorylation leads to cell growth and differentiation. External stimuli such as endotoxins, UV irradiation, heat, and hyperosmolarity, induce an array of cellular responses that culminate with gene expression. These processes are controlled by the MKK3/p38MAP kinase cascade. A third pathway responsive to environmental stress, cell death, or apoptosis is regulated by SEK/SAPKs. When overexpresssed, MEKK3 is a kinase that is capable of activating both the ERK and SAPK cascades. MEKK3 is 626 amino acids long and is ubiquitously expressed. It is positioned upstream of SEK and MEK in the signaling pathways and directly phosphorylates these enzymes. Overexpression of MEKK3 results in the activation of NFkappaB. Thus, MEKK3 represents an emerging family of kinases that is capable of inducing more than one signaling pathway. This indicates that specificity in the signaling cascades is probably achieved by effector kinases such as the ERKs and SAPKs.
Molecular Weight 71 kDa
Research Area Immunology, Innate Immunity, Kinases/Phosphatases
Pathways MAPK Signaling

Application Details

Product Details anti-MAP3K3 Antibody Target Details MAP3K3 Handling References for anti-MAP3K3 Antibody (ABIN968365) Images back to top
Comment

Related Products: ABIN967389, ABIN968535

Restrictions For Research Use only

Handling

Product Details anti-MAP3K3 Antibody Target Details MAP3K3 Application Details References for anti-MAP3K3 Antibody (ABIN968365) Images back to top
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20°C.

References for anti-MAP3K3 Antibody (ABIN968365)

Product Details anti-MAP3K3 Antibody Target Details MAP3K3 Application Details Handling Images back to top
Product cited in:

Yang, Lai, Lai, Hsu, Kuo, Yu, Lin, Yang, Kuo, Wu, Chung: "Phenethyl isothiocyanate inhibits migration and invasion of human gastric cancer AGS cells through suppressing MAPK and NF-kappaB signal pathways." in: Anticancer research, Vol. 30, Issue 6, pp. 2135-43, 2010

Ellinger-Ziegelbauer, Brown, Kelly, Siebenlist et al.: "Direct activation of the stress-activated protein kinase (SAPK) and extracellular signal-regulated protein kinase (ERK) pathways by an inducible mitogen-activated protein Kinase/ERK kinase kinase 3 ..." in: The Journal of biological chemistry, Vol. 272, Issue 5, pp. 2668-74, 1997

Images

Product Details anti-MAP3K3 Antibody Target Details MAP3K3 Application Details Handling References for anti-MAP3K3 Antibody (ABIN968365) back to top
Supplier Images
Western Blotting (WB) image for anti-MAP3K3 antibody (Mitogen-Activated Protein Kinase Kinase Kinase 3) (AA 27-135) (ABIN968365) Western blot analysis of MEKK3 on a HeLa cell lysate (Human cervical epitheloid carci...
Immunofluorescence (IF) image for anti-MAP3K3 antibody (Mitogen-Activated Protein Kinase Kinase Kinase 3) (AA 27-135) (ABIN968365) Immunofluorescence staining of RSV-3T3 cells.