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anti-Human MAP3K5 antibody for Enzyme Immunoassay

Recommended MAP3K5 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) Antibodies
  • 7420452D20Rik
  • ASK
  • ASK1
  • MAP3K5
  • MAPKKK5
  • MEKK5
  • Mekk5
  • mitogen-activated protein kinase kinase kinase 5
  • MUD21.11
  • MUD21_11
Epitope
Ser1033
53
48
43
23
22
21
17
15
13
13
13
9
8
6
5
4
4
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
374
140
78
4
1
Host
Rabbit
359
23
Clonality
Polyclonal
Conjugate
This MAP3K5 antibody is un-conjugated
13
13
12
7
7
7
5
5
5
5
5
5
5
5
5
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
287
157
136
58
55
40
23
10
8
7
6
2
1
1
1
Supplier
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Catalog No. ABIN360294
$ 401.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.000880999 ABIN360295 EIA WB Rabbit Ig Ser83 Log in to see Polyclonal 3
0.000880999 ABIN499345 EIA IF WB Rabbit IgG C-Term Log in to see Polyclonal 3
0.000880999 ABIN499349 EIA WB Rabbit IgG N-Term Log in to see Polyclonal 2
0.000880999 ABIN499347 EIA IF WB Rabbit IgG Log in to see Polyclonal 3
0.000880999 ABIN265316 EIA IHC (p) WB Rabbit Log in to see Polyclonal
0.000880999 ABIN318809 EIA WB Rabbit IgG AA 76-87, pSer83 Log in to see Polyclonal 1
0.000880999 ABIN1108200 EIA IF IHC (p) WB Mouse IgG1 Log in to see 2-00E-004 1
0.000880999 ABIN615252 EIA IF IHC (p) WB Rabbit C-Term Log in to see Polyclonal
0.000880999 ABIN317961 EIA IHC (p) WB Rabbit pSer83 Log in to see Polyclonal
0.000880999 ABIN615253 EIA IHC (p) WB Rabbit N-Term Log in to see Polyclonal

Top referenced anti-MAP3K5 antibody for Enzyme Immunoassay

Similar anti-MAP3K5 Antibodies

Application / Reactivity Human
ELISA 132 Antibodies
Enzyme Immunoassay (EIA) 11 Antibodies
Flow Cytometry (FACS) 8 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 23 Antibodies
Immunofluorescence (IF) 41 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 55 Antibodies
Immunohistochemistry (IHC) 158 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 5 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 59 Antibodies
Immunoprecipitation (IP) 8 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Radioimmunoassay (RIA) 1 Antibodies
Western Blotting (WB) 284 Antibodies

General

Antigen Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) Antibodies
Epitope Ser1033
(53), (48), (43), (23), (22), (21), (17), (15), (13), (13), (13), (9), (8), (6), (5), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(374), (140), (78), (4), (1)
Host Rabbit
(359), (23)
Clonality
Polyclonal
Conjugate This MAP3K5 antibody is un-conjugated
(13), (13), (12), (7), (7), (7), (5), (5), (5), (5), (5), (5), (5), (5), (5)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(287), (157), (136), (58), (55), (40), (23), (10), (8), (7), (6), (2), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-MAP3K5 Antibody

Target Details MAP3K5 Application Details Handling References for anti-MAP3K5 Antibody (ABIN360294) Images
Specificity This antibody reacts to ASK1 (MEKK5/MAP3K5).
Purification Affinity chromatography on Protein A
Immunogen KLH conjugated synthetic peptide corresponding to amino acid residues surrounding S83 of human MAP3K5.
Isotype Ig

Target Details MAP3K5

Product Details anti-MAP3K5 Antibody Application Details Handling References for anti-MAP3K5 Antibody (ABIN360294) Images back to top
Antigen
Alternative Name MEKK5 / ASK1 (MAP3K5 Antibody Abstract)
Background Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells, MAPKKK5 does not activate MAPK/ERK.Synonyms: Apoptosis signal-regulating kinase 1, MAP3K5, MAPK/ERK kinase kinase 5, MAPKKK5, Mitogen-activated protein kinase kinase kinase 5
Gene ID 4217, 9606
UniProt Q99683
Pathways MAPK Signaling

Application Details

Product Details anti-MAP3K5 Antibody Target Details MAP3K5 Handling References for anti-MAP3K5 Antibody (ABIN360294) Images back to top
Application Notes ELISA: 1/1,000. Western blotting: 1/50 - 1/100. Immunohistochemistry: 1/10 - 1/50.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-MAP3K5 Antibody Target Details MAP3K5 Application Details References for anti-MAP3K5 Antibody (ABIN360294) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-MAP3K5 Antibody (ABIN360294)

Product Details anti-MAP3K5 Antibody Target Details MAP3K5 Application Details Handling Images back to top
Background publications

Min, Lin, Tang, Yu, Zhang, Wan, Luhn, Fu, Chen: "AIP1 recruits phosphatase PP2A to ASK1 in tumor necrosis factor-induced ASK1-JNK activation." in: Circulation research, Vol. 102, Issue 7, pp. 840-8, 2008

Li, Luo, Yu, Lin, Luo, Zhang, He, Kim, Kim, Tang, Min: "SENP1 mediates TNF-induced desumoylation and cytoplasmic translocation of HIPK1 to enhance ASK1-dependent apoptosis." in: Cell death and differentiation, Vol. 15, Issue 4, pp. 739-50, 2008

Noguchi, Ishii, Fukutomi, Naguro, Matsuzawa, Takeda, Ichijo: "Requirement of reactive oxygen species-dependent activation of ASK1-p38 MAPK pathway for extracellular ATP-induced apoptosis in macrophage." in: The Journal of biological chemistry, Vol. 283, Issue 12, pp. 7657-65, 2008

Images

Product Details anti-MAP3K5 Antibody Target Details MAP3K5 Application Details Handling References for anti-MAP3K5 Antibody (ABIN360294) back to top
Supplier Images
 image for anti-MAP3K5 antibody (Mitogen-Activated Protein Kinase Kinase Kinase 5) (Ser1033) (ABIN360294) anti-Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) (Ser1033) antibody
 image for anti-MAP3K5 antibody (Mitogen-Activated Protein Kinase Kinase Kinase 5) (Ser1033) (ABIN360294) anti-Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) (Ser1033) antibody (Image 2)