anti-Human MAP3K5 antibody for Enzyme Immunoassay

Recommended MAP3K5 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) Antibodies
  • 7420452D20Rik
  • ASK
  • ASK1
  • MAPKKK5
  • Mekk5
  • mitogen-activated protein kinase kinase kinase 5
  • MUD21.11
  • MUD21_11
  • MEKK5
  • MAP3K5
  • mitogen-activated protein kinase kinase kinase 5
  • Map3k5
  • MAPKKK5
  • MAP3K5
Reactivity
Human, Mouse (Murine)
382
169
96
6
1
1
1
1
1
1
Host
Rabbit
362
26
Clonality
Polyclonal
Conjugate
This MAP3K5 antibody is un-conjugated
12
12
11
6
6
6
5
5
5
5
5
5
5
5
5
5
5
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
287
146
127
80
65
41
20
10
10
9
4
3
2
1
1
Supplier
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Catalog No. ABIN265316
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN318809 EIA WB Rabbit IgG AA 76-87, pSer83 Log in to see Polyclonal 1
1 ABIN5554566 EIA IF WB Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN1108200 EIA IF IHC (p) WB Mouse IgG1 Log in to see 2-00E-004 1
1 ABIN5554567 EIA IF WB Rabbit IgG Log in to see Polyclonal
1 ABIN5554568 EIA WB Rabbit IgG N-Term Log in to see Polyclonal
1 ABIN615252 EIA IF IHC (p) WB Rabbit C-Term Log in to see Polyclonal
1 ABIN317961 EIA IHC (p) WB Rabbit pSer83 Log in to see Polyclonal
1 ABIN615253 EIA IHC (p) WB Rabbit N-Term Log in to see Polyclonal
1 ABIN5541032 EIA IHC (p) WB Rabbit AA 43-90 Log in to see Polyclonal

Top referenced anti-MAP3K5 antibody for Enzyme Immunoassay

Similar anti-MAP3K5 Antibodies

Application / Reactivity Mouse (Murine) Human
ELISA 32 Antibodies 123 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies 10 Antibodies
Immunocytochemistry (ICC) 9 Antibodies 19 Antibodies
Immunofluorescence (IF) 12 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 2 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 52 Antibodies
Immunohistochemistry (IHC) 54 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 36 Antibodies
Immunoprecipitation (IP) 1 Antibodies
Western Blotting (WB) 102 Antibodies
Flow Cytometry (FACS) 10 Antibodies
Immunochromatography (IC) 2 Antibodies

General

Antigen Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) Antibodies
Reactivity Human, Mouse (Murine)
(382), (169), (96), (6), (1), (1), (1), (1), (1), (1)
Host Rabbit
(362), (26)
Clonality
Polyclonal
Conjugate This MAP3K5 antibody is un-conjugated
(12), (12), (11), (6), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(287), (146), (127), (80), (65), (41), (20), (10), (10), (9), (4), (3), (2), (1), (1)
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Product Details anti-MAP3K5 Antibody

Target Details MAP3K5 Application Details Handling Images
Specificity This antibody detects endogenous levels of MEKK5 / ASK1 protein(region surrounding Glu960).
Cross-Reactivity (Details) Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
Purification Affinity chromatography using epitope-specific immunogen.

Target Details MAP3K5

Product Details anti-MAP3K5 Antibody Application Details Handling Images back to top
Antigen
Alternative Name MEKK5 / ASK1 (MAP3K5 Antibody Abstract)
Background Mitogen-activated protein (MAP) kinase cascades are activated by various extracellular stimuli including growth factors. The MEK kinases (also called MAP kinase kinase kinases) phosphorylate and activate the MAP kinases, including ERK, JNK and p38. The MEK kinases characterized to date include Raf-1, Raf-B, MOS, MEK kinase-1, MEK kinase-2, MEK kinase-3, MEK kinase-4 and ASK 1 (also designated MEK kinase-5). MEK kinase-1 has been shown to phosphorylate MEK-1 via a Raf-independent pathway. Evidence suggests that MEK-3 is preferentially activated by MEK kinase-3 and that MEK-4 is activated by both MEK kinase-2 and MEK kinase-3. MEK kinase-4 has been shown to specifically activate the JNK pathway. ASK1 activates both MEK-4 and MEK-3/MEK-6 pathways.Synonyms: Apoptosis signal-regulating kinase 1, MAP3K5, MAPK/ERK kinase kinase 5, MAPKKK5, Mitogen-activated protein kinase kinase kinase 5
Molecular Weight approx. 155 kDa
Gene ID 4217
NCBI Accession NP_005914
UniProt Q99683
Pathways MAPK Signaling, Positive Regulation of Endopeptidase Activity

Application Details

Product Details anti-MAP3K5 Antibody Target Details MAP3K5 Handling Images back to top
Application Notes ELISA: 1/20000approx. 1/40000. Western Blot: 1/500approx. 1/1000. Immunohistochemistry: 1/50approx. 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-MAP3K5 Antibody Target Details MAP3K5 Application Details Images back to top
Concentration 1.0 mg/mL
Buffer Phosphate buffered saline (PBS), pH 7.2, 0.05 % Sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Images

Product Details anti-MAP3K5 Antibody Target Details MAP3K5 Application Details Handling back to top
Supplier Images
 image for anti-MAP3K5 antibody (Mitogen-Activated Protein Kinase Kinase Kinase 5) (ABIN265316) anti-Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) antibody