anti-Human MAP3K5 antibody for Enzyme Immunoassay

Recommended MAP3K5 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) Antibodies
  • 7420452D20Rik
  • ASK
  • ASK1
  • MAPKKK5
  • Mekk5
  • mitogen-activated protein kinase kinase kinase 5
  • MUD21.11
  • MUD21_11
  • MEKK5
  • MAP3K5
  • mitogen-activated protein kinase kinase kinase 5
  • Map3k5
  • MAPKKK5
  • MAP3K5
Epitope
Ser83
72
59
37
19
19
15
15
15
14
13
10
6
6
6
5
5
4
4
4
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Human
400
172
97
6
1
1
1
1
1
1
Host
Rabbit
379
27
Clonality
Polyclonal
Conjugate
This MAP3K5 antibody is un-conjugated
12
12
11
6
6
6
5
5
5
5
5
5
5
5
5
5
5
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
304
160
134
82
65
43
21
13
11
10
4
3
2
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

Submit your validation data for this product and get a full refund. I want to validate this product

Learn more

Catalog No. ABIN5610982
$ 357.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN5610983 EIA IHC (p) WB Rabbit Ig Fraction Ser1033 Log in to see Polyclonal
1 ABIN265316 EIA IHC (p) WB Rabbit Log in to see Polyclonal
1 ABIN318809 EIA WB Rabbit IgG AA 76-87, pSer83 Log in to see Polyclonal 1
1 ABIN1108200 EIA IF IHC (p) WB Mouse IgG1 Log in to see 2-00E-004 1
1 ABIN5554566 EIA IF WB Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN5554568 EIA WB Rabbit IgG N-Term Log in to see Polyclonal
1 ABIN5554567 EIA IF WB Rabbit IgG Log in to see Polyclonal
1 ABIN615252 EIA IF IHC (p) WB Rabbit C-Term Log in to see Polyclonal
1 ABIN615253 EIA IHC (p) WB Rabbit N-Term Log in to see Polyclonal
1 ABIN317961 EIA IHC (p) WB Rabbit pSer83 Log in to see Polyclonal
1 ABIN5541032 EIA IHC (p) WB Rabbit AA 43-90 Log in to see Polyclonal

General

Antigen Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) Antibodies
Epitope Ser83
(72), (59), (37), (19), (19), (15), (15), (15), (14), (13), (10), (6), (6), (6), (5), (5), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(400), (172), (97), (6), (1), (1), (1), (1), (1), (1)
Host Rabbit
(379), (27)
Clonality
Polyclonal
Conjugate This MAP3K5 antibody is un-conjugated
(12), (12), (11), (6), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(304), (160), (134), (82), (65), (43), (21), (13), (11), (10), (4), (3), (2), (1), (1)
Supplier Log in to see

Product Details anti-MAP3K5 Antibody

Target Details MAP3K5 Application Details Handling Images
Specificity This antibody reacts to ASK1 (MEKK5/MAP3K5) around Ser966.
Purification Protein A column, followed by peptide affinity purification
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding S966 of human MAP3K5.
Isotype Ig Fraction

Target Details MAP3K5

Product Details anti-MAP3K5 Antibody Application Details Handling Images back to top
Antigen
Alternative Name MEKK5 / ASK1 (MAP3K5 Antibody Abstract)
Background Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells, MAPKKK5 does not activate MAPK/ERK.Synonyms: Apoptosis signal-regulating kinase 1, MAP3K5, MAPK/ERK kinase kinase 5, MAPKKK5, Mitogen-activated protein kinase kinase kinase 5
Gene ID 4217, 9606
UniProt Q99683
Pathways MAPK Signaling, Positive Regulation of Endopeptidase Activity, Unfolded protein response

Application Details

Product Details anti-MAP3K5 Antibody Target Details MAP3K5 Handling Images back to top
Application Notes ELISA: 1/1,000. Western blotting: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-MAP3K5 Antibody Target Details MAP3K5 Application Details Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.