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anti-Human MAP3K5 antibody for Flow Cytometry

Recommended MAP3K5 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) Antibodies
  • 7420452D20Rik
  • ASK
  • ASK1
  • MAP3K5
  • MAPKKK5
  • MEKK5
  • Mekk5
  • mitogen-activated protein kinase kinase kinase 5
  • MUD21.11
  • MUD21_11
Reactivity
Human
374
140
78
4
1
Host
Mouse
360
22
Clonality
Monoclonal
Conjugate
This MAP3K5 antibody is un-conjugated
13
13
12
7
7
7
5
5
5
5
5
5
5
5
5
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
287
156
135
59
55
40
22
11
8
6
6
2
1
1
1
Supplier
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Catalog No. ABIN969273
$ 430.10
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.30226976 ABIN1844371 ICC FACS IF IHC ELISA WB Mouse IgG1 Log in to see
0.0010482287 ABIN189046 ICC FACS IF IHC (p) IHC WB Rabbit IgG N-Term Log in to see EP553Y
0.0010482287 ABIN1496745 ICC FACS IF IHC ELISA WB Mouse IgG1 Log in to see Immu546
0.0010482287 ABIN966515 FACS IF IHC ELISA WB Mouse IgG1 Log in to see 2E4 3
0.0010482287 ABIN865435 FACS IF IHC ELISA WB Mouse IgG1 Log in to see 2E4
0.0010482287 ABIN2307001 FACS IHC ELISA WB Mouse IgG1 Log in to see 13B500
0.0010482287 ABIN4281875 ELISA FACS ICC IF IHC WB Mouse IgG1 Log in to see 2-00E04

Top referenced anti-MAP3K5 antibody for Flow Cytometry

Similar anti-MAP3K5 Antibodies

Application / Reactivity Human
ELISA 132 Antibodies
Enzyme Immunoassay (EIA) 11 Antibodies
Flow Cytometry (FACS) 8 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 23 Antibodies
Immunofluorescence (IF) 41 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 55 Antibodies
Immunohistochemistry (IHC) 158 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 5 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 59 Antibodies
Immunoprecipitation (IP) 8 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Radioimmunoassay (RIA) 1 Antibodies
Western Blotting (WB) 284 Antibodies

General

Antigen Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) Antibodies
Reactivity Human
(374), (140), (78), (4), (1)
Host Mouse
(360), (22)
Clonality (Clone)
Monoclonal   ( )
Conjugate This MAP3K5 antibody is un-conjugated
(13), (13), (12), (7), (7), (7), (5), (5), (5), (5), (5), (5), (5), (5), (5)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(287), (156), (135), (59), (55), (40), (22), (11), (8), (6), (6), (2), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-MAP3K5 Antibody

Target Details MAP3K5 Application Details Handling References for anti-MAP3K5 Antibody (ABIN969273) Images
Purification Ascites
Immunogen Purified recombinant fragment of human MAP3K5 expressed in E. coli.
Clone 2-00E-04
Isotype IgG1

Target Details MAP3K5

Product Details anti-MAP3K5 Antibody Application Details Handling References for anti-MAP3K5 Antibody (ABIN969273) Images back to top
Antigen
Alternative Name MAP3K5 (MAP3K5 Antibody Abstract)
Background Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells, MAPKKK5 does not activate MAPK/ERK.
Molecular Weight 55 kDa
Gene ID 4217
HGNC 4217
Pathways MAPK Signaling

Application Details

Product Details anti-MAP3K5 Antibody Target Details MAP3K5 Handling References for anti-MAP3K5 Antibody (ABIN969273) Images back to top
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000, IHC: 1/200 - 1/1000, ICC: 1/200 - 1/1000, FCM: 1/200 - 1/400
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only

Handling

Product Details anti-MAP3K5 Antibody Target Details MAP3K5 Application Details References for anti-MAP3K5 Antibody (ABIN969273) Images back to top
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.

References for anti-MAP3K5 Antibody (ABIN969273)

Product Details anti-MAP3K5 Antibody Target Details MAP3K5 Application Details Handling Images back to top
Product cited in:

Widenmaier, Ao, Kim, Warnock, McIntosh et al.: "Suppression of p38 MAPK and JNK via Akt-mediated inhibition of apoptosis signal-regulating kinase 1 constitutes a core component of the beta-cell pro-survival effects of glucose-dependent ..." in: The Journal of biological chemistry, Vol. 284, Issue 44, pp. 30372-82, 2009

Images

Product Details anti-MAP3K5 Antibody Target Details MAP3K5 Application Details Handling References for anti-MAP3K5 Antibody (ABIN969273) back to top
Supplier Images
ELISA image for anti-MAP3K5 antibody (Mitogen-Activated Protein Kinase Kinase Kinase 5) (ABIN969273) Figure 1: Red: Control Antigen (100 ng), Purple: Antigen (10ng), Green: Antigen (50 n...
Flow Cytometry (FACS) image for anti-MAP3K5 antibody (Mitogen-Activated Protein Kinase Kinase Kinase 5) (ABIN969273) Figure 2: Flow cytometric analysis of Hela cells using MAP3K5 mouse mAb (green) and n...
Immunocytochemistry (ICC) image for anti-MAP3K5 antibody (Mitogen-Activated Protein Kinase Kinase Kinase 5) (ABIN969273) Figure 3: Immunofluorescence analysis of Hela cells using MAP3K5 mouse mAb (green) . ...
Immunohistochemistry (IHC) image for anti-MAP3K5 antibody (Mitogen-Activated Protein Kinase Kinase Kinase 5) (ABIN969273) Figure 4: Immunohistochemical analysis of paraffin-embedded breast cancer tissues (le...
Western Blotting (WB) image for anti-MAP3K5 antibody (Mitogen-Activated Protein Kinase Kinase Kinase 5) (ABIN969273) Figure 5: Western blot analysis using MAP3K5 mAb against human MAP3K5 (AA: 922-1108) ...