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|Application / Reactivity||Human|
|Enzyme Immunoassay (EIA)||11 Antibodies|
|Flow Cytometry (FACS)||7 Antibodies|
|Immunochromatography (IC)||2 Antibodies|
|Immunocytochemistry (ICC)||20 Antibodies|
|Immunofluorescence (IF)||38 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||56 Antibodies|
|Immunohistochemistry (IHC)||142 Antibodies|
|Immunohistochemistry (Acetone-fixed) (IHC (af))||1 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||5 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||50 Antibodies|
|Immunoprecipitation (IP)||6 Antibodies|
|Proximity Ligation Assay (PLA)||1 Antibodies|
|Radioimmunoassay (RIA)||1 Antibodies|
|Western Blotting (WB)||276 Antibodies|
|Antigen||Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) Antibodies|
|Conjugate||This MAP3K5 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
|3 references available|
|Supplier||Log in to see|
Product Details anti-MAP3K5 AntibodyTarget Details MAP3K5 Application Details Handling References for anti-MAP3K5 Antibody (ABIN499345) Images
|Specificity||ASK1 antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human ASK1 This sequence is different from that of mouse by last two amino acids.|
|Purification||Affinity chromatography purified via peptide column|
|Immunogen||Human ASK1 Peptide|
Target Details MAP3K5Product Details anti-MAP3K5 Antibody Application Details Handling References for anti-MAP3K5 Antibody (ABIN499345) Images back to top
|Alternative Name||MEKK5 / ASK1 (MAP3K5 Antibody Abstract)|
|Background||Mitogen-activated protein (MAP) kinase cascades are activated in response to various extracellular stimuli, including cytokines, growth factors and environmental stresses. A novel MAP kinase kinase kinase (MAPKKK) was recently identified and designated ASK1 (for apoptosis signal-regulating kinase 1) and MAPKKK5. ASK1 activated two different subgroups of MAPKK, MKK4 and MKK6, which in turn activated c-Jun N-terminal kinase (JNK) and p38 MAP kinase, respectively. ASK1/MAPKKK5 is activated by TNFR and Fas through the interaction with members of the TRAF family and Fas-associated protein Daxx. Overexpression of ASK1 induced apoptotic cell death, and a catalytically inactive form of ASK1 inhibited TNF-alpha-induced apoptosis. ASK1 is expressed in variety of human and mouse tissues.Synonyms: Apoptosis signal-regulating kinase 1, MAP3K5, MAPK/ERK kinase kinase 5, MAPKKK5, Mitogen-activated protein kinase kinase kinase 5|
Application DetailsProduct Details anti-MAP3K5 Antibody Target Details MAP3K5 Handling References for anti-MAP3K5 Antibody (ABIN499345) Images back to top
ELISA. Western Blot: ASK1 antibody can be used for detection of ASK1 at 1: 500 to 1: 1000 dilution. A 155 kDa band can be detected. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-MAP3K5 Antibody Target Details MAP3K5 Application Details References for anti-MAP3K5 Antibody (ABIN499345) Images back to top
|Buffer||PBS containing 0.02 % Sodium Azide as preservative|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
References for anti-MAP3K5 Antibody (ABIN499345)Product Details anti-MAP3K5 Antibody Target Details MAP3K5 Application Details Handling Images back to top
Tobiume, Inage, Takeda et al.: "Molecular cloning and characterization of the mouse apoptosis signal-regulating kinase 1." in: Biochemical and biophysical research communications, Vol. 239, Issue 3, pp. 905-10, 1997 (PubMed).
Ichijo, Nishida, Irie et al.: "Induction of apoptosis by ASK1, a mammalian MAPKKK that activates SAPK/JNK and p38 signaling pathways." in: Science (New York, N.Y.), Vol. 275, Issue 5296, pp. 90-4, 1997 (PubMed).
Wang, Diener, Jannuzzi et al.: "Molecular cloning and characterization of a novel protein kinase with a catalytic domain homologous to mitogen-activated protein kinase kinase kinase." in: The Journal of biological chemistry, Vol. 271, Issue 49, pp. 31607-11, 1997 (PubMed).
ImagesProduct Details anti-MAP3K5 Antibody Target Details MAP3K5 Application Details Handling References for anti-MAP3K5 Antibody (ABIN499345) back to top