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anti-Human MAP3K5 antibody for Immunofluorescence

Recommended MAP3K5 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) Antibodies
  • 7420452D20Rik
  • ASK
  • ASK1
  • MAP3K5
  • MAPKKK5
  • MEKK5
  • Mekk5
  • mitogen-activated protein kinase kinase kinase 5
  • MUD21.11
  • MUD21_11
Epitope
C-Term
52
46
43
23
22
19
17
15
13
13
13
9
8
6
5
4
4
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
364
137
77
4
1
Host
Rabbit
350
22
Clonality
Polyclonal
Conjugate
This MAP3K5 antibody is un-conjugated
13
13
12
7
7
7
5
5
5
5
5
5
5
5
5
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
277
147
132
55
50
36
20
10
6
6
6
2
1
1
1
Supplier
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Catalog No. ABIN499345
$ 401.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.3157438 ABIN499347 EIA IF WB Rabbit IgG Log in to see Polyclonal 3
0.3157438 ABIN1108200 EIA IF IHC (p) WB Mouse IgG1 Log in to see 2-00E-004 1
0.3157438 ABIN189046 ICC FACS IF IHC (p) IHC WB Rabbit IgG N-Term Log in to see EP553Y
0.3157438 ABIN615252 EIA IF IHC (p) WB Rabbit C-Term Log in to see Polyclonal
0.3157438 ABIN517859 IF ELISA WB Mouse IgG Mix lambda AA 1231-1374, partial Log in to see 2D11
0.3157438 ABIN2425157 IF IHC WB Rabbit IgG Log in to see Polyclonal
0.0010826217 ABIN393594 IF WB Mouse IgG1 lambda AA 1231-1374 Log in to see 2D11 5
0.0010826217 ABIN197326 IF IHC (p) WB Rabbit Ser966 Log in to see Polyclonal 4
0.0010826217 ABIN197310 IF IHC (p) WB Rabbit Ser83 Log in to see Polyclonal 3
0.0010826217 ABIN362730 IF IHC Rabbit IgG AA 964-968 Log in to see Polyclonal 5
0.0010826217 ABIN362714 IF IHC WB Rabbit IgG AA 81-85 Log in to see Polyclonal 4
0.0010826217 ABIN318975 IHC (fro) IF WB Rabbit IgG C-Term Log in to see Polyclonal
0.0010826217 ABIN1496745 ICC FACS IF IHC ELISA WB Mouse IgG1 Log in to see Immu546
0.0010826217 ABIN2162756 IF IHC Rabbit IgG Ser966 Log in to see Polyclonal
0.0010826217 ABIN966515 FACS IF IHC ELISA WB Mouse IgG1 Log in to see 2E4 3
0.0010826217 ABIN222944 IF WB Rabbit IgG Log in to see Polyclonal
0.0010826217 ABIN241667 IF WB Rabbit Log in to see Polyclonal
0.0010826217 ABIN1731994 ICC IF IP WB Rabbit IgG C-Term Log in to see Polyclonal
0.0010826217 ABIN865435 FACS IF IHC ELISA WB Mouse IgG1 Log in to see 2E4
0.0010826217 ABIN1842390 IF IHC Rabbit IgG pSer966 Log in to see Polyclonal

Top referenced anti-MAP3K5 antibody for Immunofluorescence

Similar anti-MAP3K5 Antibodies

Application / Reactivity Human
ELISA 128 Antibodies
Enzyme Immunoassay (EIA) 11 Antibodies
Flow Cytometry (FACS) 7 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 20 Antibodies
Immunofluorescence (IF) 38 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 55 Antibodies
Immunohistochemistry (IHC) 148 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 5 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 50 Antibodies
Immunoprecipitation (IP) 6 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Radioimmunoassay (RIA) 1 Antibodies
Western Blotting (WB) 274 Antibodies

General

Antigen Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) Antibodies
Epitope C-Term
(52), (46), (43), (23), (22), (19), (17), (15), (13), (13), (13), (9), (8), (6), (5), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(364), (137), (77), (4), (1)
Host Rabbit
(350), (22)
Clonality
Polyclonal
Conjugate This MAP3K5 antibody is un-conjugated
(13), (13), (12), (7), (7), (7), (5), (5), (5), (5), (5), (5), (5), (5), (5)
Application Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
(277), (147), (132), (55), (50), (36), (20), (10), (6), (6), (6), (2), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-MAP3K5 Antibody

Target Details MAP3K5 Application Details Handling References for anti-MAP3K5 Antibody (ABIN499345) Images
Specificity ASK1 antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human ASK1 This sequence is different from that of mouse by last two amino acids.
Purification Affinity chromatography purified via peptide column
Immunogen Human ASK1 Peptide
Isotype IgG

Target Details MAP3K5

Product Details anti-MAP3K5 Antibody Application Details Handling References for anti-MAP3K5 Antibody (ABIN499345) Images back to top
Antigen
Alternative Name MEKK5 / ASK1 (MAP3K5 Antibody Abstract)
Background Mitogen-activated protein (MAP) kinase cascades are activated in response to various extracellular stimuli, including cytokines, growth factors and environmental stresses. A novel MAP kinase kinase kinase (MAPKKK) was recently identified and designated ASK1 (for apoptosis signal-regulating kinase 1) and MAPKKK5. ASK1 activated two different subgroups of MAPKK, MKK4 and MKK6, which in turn activated c-Jun N-terminal kinase (JNK) and p38 MAP kinase, respectively. ASK1/MAPKKK5 is activated by TNFR and Fas through the interaction with members of the TRAF family and Fas-associated protein Daxx. Overexpression of ASK1 induced apoptotic cell death, and a catalytically inactive form of ASK1 inhibited TNF-alpha-induced apoptosis. ASK1 is expressed in variety of human and mouse tissues.Synonyms: Apoptosis signal-regulating kinase 1, MAP3K5, MAPK/ERK kinase kinase 5, MAPKKK5, Mitogen-activated protein kinase kinase kinase 5
Gene ID 4217
UniProt Q99683
Pathways MAPK Signaling

Application Details

Product Details anti-MAP3K5 Antibody Target Details MAP3K5 Handling References for anti-MAP3K5 Antibody (ABIN499345) Images back to top
Application Notes ELISA. Western Blot: ASK1 antibody can be used for detection of ASK1 at 1: 500 to 1: 1000 dilution. A 155 kDa band can be detected. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-MAP3K5 Antibody Target Details MAP3K5 Application Details References for anti-MAP3K5 Antibody (ABIN499345) Images back to top
Buffer PBS containing 0.02 % Sodium Azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-MAP3K5 Antibody (ABIN499345)

Product Details anti-MAP3K5 Antibody Target Details MAP3K5 Application Details Handling Images back to top
Background publications

Ichijo, Nishida, Irie, ten Dijke, Saitoh, Moriguchi, Takagi, Matsumoto, Miyazono, Gotoh: "Induction of apoptosis by ASK1, a mammalian MAPKKK that activates SAPK/JNK and p38 signaling pathways." in: Science (New York, N.Y.), Vol. 275, Issue 5296, pp. 90-4, 1997

Wang, Diener, Jannuzzi, Trollinger, Tan, Lichenstein, Zukowski, Yao: "Molecular cloning and characterization of a novel protein kinase with a catalytic domain homologous to mitogen-activated protein kinase kinase kinase." in: The Journal of biological chemistry, Vol. 271, Issue 49, pp. 31607-11, 1997

Tobiume, Inage, Takeda, Enomoto, Miyazono, Ichijo: "Molecular cloning and characterization of the mouse apoptosis signal-regulating kinase 1." in: Biochemical and biophysical research communications, Vol. 239, Issue 3, pp. 905-10, 1997

Images

Product Details anti-MAP3K5 Antibody Target Details MAP3K5 Application Details Handling References for anti-MAP3K5 Antibody (ABIN499345) back to top
Supplier Images
Immunofluorescence (IF) image for anti-MAP3K5 antibody (Mitogen-Activated Protein Kinase Kinase Kinase 5) (C-Term) (ABIN499345) Immunocytochemistry of ASK1 in A431 cells with AP30085PU-N ASK1 antibody at 10 μg/ml.
Immunofluorescence (IF) image for anti-MAP3K5 antibody (Mitogen-Activated Protein Kinase Kinase Kinase 5) (C-Term) (ABIN499345) Immunofluorescence of ASK1 in A431 cells with AP30085PU-N ASK1 antibody at 20 μg/ml.
Western Blotting (WB) image for anti-MAP3K5 antibody (Mitogen-Activated Protein Kinase Kinase Kinase 5) (C-Term) (ABIN499345) Western blot analysis of ASK1 in SW1353 whole cell lysate with AP30085PU-N ASK1 antib...