You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Human MAP3K5 antibody for Radioimmunoassay

Recommended MAP3K5 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) Antibodies
  • 7420452D20Rik
  • ASK
  • ASK1
  • MAPKKK5
  • Mekk5
  • mitogen-activated protein kinase kinase kinase 5
  • MUD21.11
  • MUD21_11
  • MEKK5
  • MAP3K5
  • mitogen-activated protein kinase kinase kinase 5
  • Map3k5
  • MAPKKK5
  • MAP3K5
Epitope
AA 76-87, pSer83
56
45
36
19
19
18
15
15
15
14
12
10
6
6
5
5
4
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
369
169
96
6
1
1
1
1
1
1
Host
Rabbit
350
25
Clonality
Polyclonal
Conjugate
This MAP3K5 antibody is un-conjugated
12
12
11
6
6
6
5
5
5
5
5
5
5
5
5
5
5
Application
Immunoprecipitation (IP), Immunohistochemistry (IHC), Radioimmunoassay (RIA), ELISA, Western Blotting (WB)
277
143
120
67
65
39
20
11
9
9
4
3
2
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN95283
$ 415.00
Plus shipping costs $45.00 and $21.00 dry ice

Similar anti-MAP3K5 Antibodies

Application / Reactivity Human
ELISA 117 Antibodies
Enzyme Immunoassay (EIA) 11 Antibodies
Flow Cytometry (FACS) 9 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 19 Antibodies
Immunofluorescence (IF) 39 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 2 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 65 Antibodies
Immunohistochemistry (IHC) 141 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 4 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 67 Antibodies
Immunoprecipitation (IP) 9 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Radioimmunoassay (RIA) 1 Antibodies
Western Blotting (WB) 272 Antibodies

General

Antigen Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) Antibodies
Epitope AA 76-87, pSer83
(56), (45), (36), (19), (19), (18), (15), (15), (15), (14), (12), (10), (6), (6), (5), (5), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(369), (169), (96), (6), (1), (1), (1), (1), (1), (1)
Host Rabbit
(350), (25)
Clonality
Polyclonal
Conjugate This MAP3K5 antibody is un-conjugated
(12), (12), (11), (6), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5)
Application Immunoprecipitation (IP), Immunohistochemistry (IHC), Radioimmunoassay (RIA), ELISA, Western Blotting (WB)
(277), (143), (120), (67), (65), (39), (20), (11), (9), (9), (4), (3), (2), (1)
Supplier Log in to see

Product Details anti-MAP3K5 Antibody

Target Details MAP3K5 Application Details Handling Images
Characteristics Concentration Definition: by UV absorbance at 280 nm
Immunogen This purified antibody was prepared from rabbit serum after repeated immunizations with a KLH conjugated peptide corresponding to amino acids 76-87 of human ASK-1 protein.
Isotype IgG

Target Details MAP3K5

Product Details anti-MAP3K5 Antibody Application Details Handling Images back to top
Antigen
Alternative Name ASK-1 (MAP3K5 Antibody Abstract)
Background ASK-1 (apoptosis signal-regulating kinase 1 - also referred to as MEK Kinase-5 or MAPKKK5) is a novel serine/threonine MAP kinase kinase kinase (MAPKKK) component of the mitogen -activated protein (MAP) cascade that is activated in response to extracellular stimuli by cytokines, growth factors and environmental stresses and other factors.   Overexpression of ASK-1 induces apoptotic cell death.  ASK-1 is expressed in a variety of human and mouse tissues.  The overall amino acid sequence identity between the mouse and human ASK1 is 91.9%.  ASK-1 interacts with CDKN1A (also known as p21, WAF1, CIP1).  Please refer to the reference list at the end of this document for further information.
Synonyms: Apoptosis signal regulating kinase 1 antibody, ASK 1 antibody, ASK1 antibody, MAP/ERK kinase kinase 5 antibody, MAP3K5 antibody, MAPK/ERK kinase kinase 5 antibody
Gene ID 4217
UniProt Q99683
Pathways MAPK Signaling, Positive Regulation of Endopeptidase Activity

Application Details

Product Details anti-MAP3K5 Antibody Target Details MAP3K5 Handling Images back to top
Application Notes This phospho specific polyclonal antibody reacts human pS83 ASK1 and shows minimal reactivity by western blot, ELISA and competitive ELISA with non-phosphorylated ASK1. Although not tested, this antibody is likely functional in RIA, immunohistochemistry and immunoprecipitation. For immunoblotting a 1:1,000 dilution is recommended.  A 155 kDa band corresponding to human ASK-1 is detected.  Whole cell lysates from SW1353 can be used as a positive control.  For ELISA a 1:5,000 to 1:10,000 dilution is recommended.   Researchers should determine optimal titers for other applications.
Restrictions For Research Use only

Handling

Product Details anti-MAP3K5 Antibody Target Details MAP3K5 Application Details Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C

Images

Product Details anti-MAP3K5 Antibody Target Details MAP3K5 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-MAP3K5 antibody (Mitogen-Activated Protein Kinase Kinase Kinase 5) (AA 76-87) (ABIN95283) Western blot of anti-pS83 ASK1 antibodies shows specificity for phosphorylated human ...
 image for anti-MAP3K5 antibody (Mitogen-Activated Protein Kinase Kinase Kinase 5) (AA 76-87) (ABIN95283) anti-Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) (AA 76-87), (pSer83) antibody (Image 2)