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anti-Human MAP3K5 antibody for Radioimmunoassay

Recommended MAP3K5 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) Antibodies
  • 7420452D20Rik
  • ASK
  • ASK1
  • MAP3K5
  • MAPKKK5
  • MEKK5
  • Mekk5
  • mitogen-activated protein kinase kinase kinase 5
  • MUD21.11
  • MUD21_11
Epitope
AA 76-87, pSer83
52
48
43
23
22
21
17
15
13
13
13
9
7
6
5
4
4
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
374
140
78
4
1
Host
Rabbit
359
23
Clonality
Polyclonal
Conjugate
This MAP3K5 antibody is un-conjugated
13
13
12
7
7
7
5
5
5
5
5
5
5
5
5
Application
Immunoprecipitation (IP), Immunohistochemistry (IHC), Radioimmunoassay (RIA), ELISA, Western Blotting (WB)
287
156
135
59
55
40
23
11
7
7
6
2
1
1
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Catalog No. ABIN95283
$ 369.00
Plus shipping costs $45.00 and $21.00 dry ice

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Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 23 Antibodies
Immunofluorescence (IF) 41 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 55 Antibodies
Immunohistochemistry (IHC) 158 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 1 Antibodies
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Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 59 Antibodies
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Proximity Ligation Assay (PLA) 1 Antibodies
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Western Blotting (WB) 284 Antibodies

General

Antigen Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) Antibodies
Epitope AA 76-87, pSer83
(52), (48), (43), (23), (22), (21), (17), (15), (13), (13), (13), (9), (7), (6), (5), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(374), (140), (78), (4), (1)
Host Rabbit
(359), (23)
Clonality
Polyclonal
Conjugate This MAP3K5 antibody is un-conjugated
(13), (13), (12), (7), (7), (7), (5), (5), (5), (5), (5), (5), (5), (5), (5)
Application Immunoprecipitation (IP), Immunohistochemistry (IHC), Radioimmunoassay (RIA), ELISA, Western Blotting (WB)
(287), (156), (135), (59), (55), (40), (23), (11), (7), (7), (6), (2), (1), (1)
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Product Details anti-MAP3K5 Antibody

Target Details MAP3K5 Application Details Handling Images
Characteristics Concentration Definition: by UV absorbance at 280 nm
Immunogen This purified antibody was prepared from rabbit serum after repeated immunizations with a KLH conjugated peptide corresponding to amino acids 76-87 of human ASK-1 protein.
Isotype IgG

Target Details MAP3K5

Product Details anti-MAP3K5 Antibody Application Details Handling Images back to top
Antigen
Alternative Name ASK-1 (MAP3K5 Antibody Abstract)
Background ASK-1 (apoptosis signal-regulating kinase 1 - also referred to as MEK Kinase-5 or MAPKKK5) is a novel serine/threonine MAP kinase kinase kinase (MAPKKK) component of the mitogen -activated protein (MAP) cascade that is activated in response to extracellular stimuli by cytokines, growth factors and environmental stresses and other factors.   Overexpression of ASK-1 induces apoptotic cell death.  ASK-1 is expressed in a variety of human and mouse tissues.  The overall amino acid sequence identity between the mouse and human ASK1 is 91.9%.  ASK-1 interacts with CDKN1A (also known as p21, WAF1, CIP1).  Please refer to the reference list at the end of this document for further information.
Synonyms: Apoptosis signal regulating kinase 1 antibody, ASK 1 antibody, ASK1 antibody, MAP/ERK kinase kinase 5 antibody, MAP3K5 antibody, MAPK/ERK kinase kinase 5 antibody
Gene ID 4217
UniProt Q99683
Pathways MAPK Signaling

Application Details

Product Details anti-MAP3K5 Antibody Target Details MAP3K5 Handling Images back to top
Application Notes This phospho specific polyclonal antibody reacts human pS83 ASK1 and shows minimal reactivity by western blot, ELISA and competitive ELISA with non-phosphorylated ASK1. Although not tested, this antibody is likely functional in RIA, immunohistochemistry and immunoprecipitation. For immunoblotting a 1:1,000 dilution is recommended.  A 155 kDa band corresponding to human ASK-1 is detected.  Whole cell lysates from SW1353 can be used as a positive control.  For ELISA a 1:5,000 to 1:10,000 dilution is recommended.   Researchers should determine optimal titers for other applications.
Restrictions For Research Use only

Handling

Product Details anti-MAP3K5 Antibody Target Details MAP3K5 Application Details Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C

Images

Product Details anti-MAP3K5 Antibody Target Details MAP3K5 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-MAP3K5 antibody (Mitogen-Activated Protein Kinase Kinase Kinase 5) (AA 76-87) (ABIN95283) Western blot of anti-pS83 ASK1 antibodies shows specificity for phosphorylated human ...
 image for anti-MAP3K5 antibody (Mitogen-Activated Protein Kinase Kinase Kinase 5) (AA 76-87) (ABIN95283) anti-Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) (AA 76-87), (pSer83) antibody (Image 2)