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anti-Mouse (Murine) MAP3K5 antibody for Flow Cytometry

Recommended MAP3K5 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) Antibodies
  • 7420452D20Rik
  • ASK
  • ASK1
  • MAP3K5
  • MAPKKK5
  • MEKK5
  • Mekk5
  • mitogen-activated protein kinase kinase kinase 5
  • MUD21.11
  • MUD21_11
Epitope
N-Term
53
48
43
23
22
21
17
15
13
13
13
9
8
6
5
4
4
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
375
140
78
4
1
Host
Rabbit
360
23
Clonality
Monoclonal
Conjugate
This MAP3K5 antibody is un-conjugated
13
13
12
7
7
7
5
5
5
5
5
5
5
5
5
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
288
157
136
59
55
40
23
11
8
7
6
2
1
1
1
Supplier
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Available images

Catalog No. ABIN189046
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Similar anti-MAP3K5 Antibodies

Application / Reactivity Mouse (Murine) Human
ELISA 24 Antibodies 132 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies 11 Antibodies
Flow Cytometry (FACS) 1 Antibodies 8 Antibodies
Immunocytochemistry (ICC) 11 Antibodies 23 Antibodies
Immunofluorescence (IF) 14 Antibodies 41 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 44 Antibodies 55 Antibodies
Immunohistochemistry (IHC) 46 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 28 Antibodies
Western Blotting (WB) 81 Antibodies
Immunochromatography (IC) 2 Antibodies

General

Antigen Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) Antibodies
Epitope N-Term
(53), (48), (43), (23), (22), (21), (17), (15), (13), (13), (13), (9), (8), (6), (5), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(375), (140), (78), (4), (1)
Host Rabbit
(360), (23)
Clonality (Clone)
Monoclonal   ( )
Conjugate This MAP3K5 antibody is un-conjugated
(13), (13), (12), (7), (7), (7), (5), (5), (5), (5), (5), (5), (5), (5), (5)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
(288), (157), (136), (59), (55), (40), (23), (11), (8), (7), (6), (2), (1), (1), (1)
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Product Details anti-MAP3K5 Antibody

Target Details MAP3K5 Application Details Handling Images
Cross-Reactivity (Details) Cross reactivity determined by Western Blot only.
Purification IgG purified
Immunogen A synthetic peptide corresponding to residues in N-terminus of human MEKK5/ASK1 was used as an immunogen.
Clone EP553Y
Isotype IgG

Target Details MAP3K5

Product Details anti-MAP3K5 Antibody Application Details Handling Images back to top
Antigen
Alternative Name MEKK5 / ASK1 (MAP3K5 Antibody Abstract)
Background Apoptosis signal-regulating kinase-1 (ASK1, MEKK5, MAPKKK5) is a MAP3k typeprotein kinase belonging to mitogen-activated protein kinase kinase kinase (MAPKKK)family. As a regulator of JNK and p38 MAPK signaling pathways, ASK1/MEKK5phosphorylates MMK4 to activate c-Jun N-terminal kinase (JNK) and phosphorylatesMEK3 and MKK6 to activate p38. ASK1/MEKK5 is activated by oxidative stress, TNF viaTRAF2, Fas via Daxx, calcium overload, and endoplasmic reticulum stress (1).ASK1/MEKK5 is inactivated by Trx, Grx, 14-3-3, and protein serine/threoninephosphatase 5 (2). While elevated level of ASK1/MEKK5 can induce apoptosis, acatalytically inactive ASK1/MEKK5 acts as an inhibitor in TNF-alpha induced apoptosis.Over-expression of ASK1/MEKK5 has also been implicated in cell differentiation, suchas in keratinocyte differentiation through p38 pathway (3). ASK1/MEKK5 has beenimplicated as a therapeutic target for neurodegenerative disorder sand cardiacdysfunction (4).
Gene Symbol: MAP3K5
Gene ID 4217, 293015
Pathways MAPK Signaling

Application Details

Product Details anti-MAP3K5 Antibody Target Details MAP3K5 Handling Images back to top
Application Notes Recommended dilutions: Flow Cytometry 1:40, Immunocytochemistry/Immunofluorescence 1:100-250, Immunohistochemistry 1:100-250, Immunohistochemistry-Paraffin 1:100-250, Western Blot 1:1000-10000
Protocol Protocol specific for ASK1 Antibody Immunohistochemistry Protocol for Paraffin-embedded Tissues
1. Solutions and reagents1.
. Xylene1.
. Ethanol, anhydrous denatured, histological grade (100 %, 95 %, 70 %)1.
. Washing buffer:TBST washing buffer: 1XTBS/0.1 % Tween-20To prepare stock solution of 10X TBS: add 24.2 g Trizma base and 80 g sodium chloride to 1L of dH2O. Adjust pH to 7.6.Working solution. 1XTBST/0.1 % Tween-20: add 100ml 10XTBS to 900 mL dH2O. Add 1 mL Tween-20 and mix well.1.
. Distilled water (dH2O)1.
. Antigen Retrieval Solution:0.01M Sodium Citrate
Restrictions For Research Use only

Handling

Product Details anti-MAP3K5 Antibody Target Details MAP3K5 Application Details Images back to top
Format Liquid
Buffer PBS 49 % , Glycerol 50 % , BSA 0.05 % , Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment -20 °C.

Images

Product Details anti-MAP3K5 Antibody Target Details MAP3K5 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-MAP3K5 antibody (Mitogen-Activated Protein Kinase Kinase Kinase 5) (N-Term) (ABIN189046) Western blot: MEKK1 Antibody (ABIN189046) - western blot on analysis on HeLa cell lys...
Immunohistochemistry (IHC) image for anti-MAP3K5 antibody (Mitogen-Activated Protein Kinase Kinase Kinase 5) (N-Term) (ABIN189046) Immunohistochemistry-Paraffin: MEKK1 Antibody (NB110-55148) - Immunohistochemical ana...
Immunofluorescence (IF) image for anti-MAP3K5 antibody (Mitogen-Activated Protein Kinase Kinase Kinase 5) (N-Term) (ABIN189046) Immunofluorescent staining of HeLa cells using anti-MEKK5 RabMAb