|Application / Reactivity||Mouse (Murine)||Human|
|ELISA||23 Antibodies||121 Antibodies|
|Enzyme Immunoassay (EIA)||3 Antibodies||11 Antibodies|
|Flow Cytometry (FACS)||1 Antibodies||7 Antibodies|
|Immunocytochemistry (ICC)||9 Antibodies||20 Antibodies|
|Immunofluorescence (IF)||12 Antibodies||38 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||45 Antibodies||56 Antibodies|
|Immunohistochemistry (IHC)||43 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||1 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||25 Antibodies|
|Western Blotting (WB)||79 Antibodies|
|Immunochromatography (IC)||2 Antibodies|
|Antigen||Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) Antibodies|
|Reactivity||Human, Mouse (Murine) Alternatives|
|Conjugate||This MAP3K5 antibody is un-conjugated Alternatives|
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
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Product Details anti-MAP3K5 AntibodyTarget Details MAP3K5 Application Details Handling Images
|Cross-Reactivity (Details)||Cross reactivity determined by Western Blot only.|
|Immunogen||A synthetic peptide corresponding to residues in N-terminus of human MEKK5/ASK1 was used as an immunogen.|
Target Details MAP3K5Product Details anti-MAP3K5 Antibody Application Details Handling Images back to top
|Alternative Name||MEKK5 / ASK1 (MAP3K5 Antibody Abstract)|
Apoptosis signal-regulating kinase-1 (ASK1, MEKK5, MAPKKK5) is a MAP3k typeprotein kinase belonging to mitogen-activated protein kinase kinase kinase (MAPKKK)family. As a regulator of JNK and p38 MAPK signaling pathways, ASK1/MEKK5phosphorylates MMK4 to activate c-Jun N-terminal kinase (JNK) and phosphorylatesMEK3 and MKK6 to activate p38. ASK1/MEKK5 is activated by oxidative stress, TNF viaTRAF2, Fas via Daxx, calcium overload, and endoplasmic reticulum stress (1).ASK1/MEKK5 is inactivated by Trx, Grx, 14-3-3, and protein serine/threoninephosphatase 5 (2). While elevated level of ASK1/MEKK5 can induce apoptosis, acatalytically inactive ASK1/MEKK5 acts as an inhibitor in TNF-alpha induced apoptosis.Over-expression of ASK1/MEKK5 has also been implicated in cell differentiation, suchas in keratinocyte differentiation through p38 pathway (3). ASK1/MEKK5 has beenimplicated as a therapeutic target for neurodegenerative disorder sand cardiacdysfunction (4).
Gene Symbol: MAP3K5
|Gene ID||4217, 293015|
Application DetailsProduct Details anti-MAP3K5 Antibody Target Details MAP3K5 Handling Images back to top
|Application Notes||Recommended dilutions: Flow Cytometry 1:40, Immunocytochemistry/Immunofluorescence 1:100-250, Immunohistochemistry 1:100-250, Immunohistochemistry-Paraffin 1:100-250, Western Blot 1:1000-10000|
Protocol specific for ASK1 Antibody Immunohistochemistry Protocol for Paraffin-embedded Tissues
1. Solutions and reagents1.
. Ethanol, anhydrous denatured, histological grade (100 %, 95 %, 70 %)1.
. Washing buffer:TBST washing buffer: 1XTBS/0.1 % Tween-20To prepare stock solution of 10X TBS: add 24.2 g Trizma base and 80 g sodium chloride to 1L of dH2O. Adjust pH to 7.6.Working solution. 1XTBST/0.1 % Tween-20: add 100ml 10XTBS to 900 mL dH2O. Add 1 mL Tween-20 and mix well.1.
. Distilled water (dH2O)1.
. Antigen Retrieval Solution:0.01M Sodium Citrate
|Restrictions||For Research Use only|
HandlingProduct Details anti-MAP3K5 Antibody Target Details MAP3K5 Application Details Images back to top
|Buffer||PBS 49 % , Glycerol 50 % , BSA 0.05 % , Sodium Azide|
|Precaution of Use||WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.|
|Handling Advice||Avoid freeze-thaw cycles|
|Storage Comment||-20 °C.|
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