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anti-Mouse (Murine) MAP3K5 antibody for Immunofluorescence

Recommended MAP3K5 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) Antibodies
  • 7420452D20Rik
  • ASK
  • ASK1
  • MAPKKK5
  • Mekk5
  • mitogen-activated protein kinase kinase kinase 5
  • MUD21.11
  • MUD21_11
  • MEKK5
  • MAP3K5
  • mitogen-activated protein kinase kinase kinase 5
  • Map3k5
  • MAPKKK5
  • MAP3K5
Reactivity
Human, Mouse (Murine)
369
168
96
6
1
1
1
1
1
1
Host
Rabbit
350
25
Clonality
Polyclonal
Conjugate
This MAP3K5 antibody is un-conjugated
12
12
11
6
6
6
5
5
5
5
5
5
5
5
5
5
5
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
277
144
121
67
65
38
20
10
10
9
4
3
2
1
1
Supplier
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Catalog No. ABIN499347
$ 401.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
11.318237 ABIN4281869 ICC IF IHC IHC (p) WB Rabbit Log in to see Polyclonal
11.318237 ABIN4281878 ELISA ICC IF IHC IHC (p) WB Rabbit C-Term Log in to see Polyclonal
1 ABIN197326 IF IHC (p) WB Rabbit Ser966 Log in to see Polyclonal 4
1 ABIN362730 IF IHC Rabbit IgG AA 964-968 Log in to see Polyclonal 5
1 ABIN1980290 IF IHC WB Rabbit IgG Log in to see Polyclonal
1 ABIN1842390 IF IHC Rabbit IgG pSer966 Log in to see Polyclonal
1 ABIN2881584 IF IHC WB Rabbit IgG Log in to see Polyclonal
1 ABIN2958733 ICC IF ELISA WB Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN273949 IF IHC Rabbit IgG Log in to see Polyclonal
1 ABIN273948 IF IHC Rabbit IgG Log in to see Polyclonal
1 ABIN1574853 IF IHC WB Rabbit IgG Log in to see Polyclonal

Top referenced anti-MAP3K5 antibody for Immunofluorescence

Similar anti-MAP3K5 Antibodies

Application / Reactivity Mouse (Murine) Human
ELISA 32 Antibodies 117 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies 11 Antibodies
Immunocytochemistry (ICC) 9 Antibodies 19 Antibodies
Immunofluorescence (IF) 12 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 2 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 52 Antibodies
Immunohistochemistry (IHC) 54 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 35 Antibodies
Immunoprecipitation (IP) 1 Antibodies
Western Blotting (WB) 101 Antibodies
Flow Cytometry (FACS) 9 Antibodies
Immunochromatography (IC) 2 Antibodies

General

Antigen Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) Antibodies
Reactivity Human, Mouse (Murine)
(369), (168), (96), (6), (1), (1), (1), (1), (1), (1)
Host Rabbit
(350), (25)
Clonality
Polyclonal
Conjugate This MAP3K5 antibody is un-conjugated
(12), (12), (11), (6), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5)
Application Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
(277), (144), (121), (67), (65), (38), (20), (10), (10), (9), (4), (3), (2), (1), (1)
Pubmed 3 references available
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Product Details anti-MAP3K5 Antibody

Target Details MAP3K5 Application Details Handling References for anti-MAP3K5 Antibody (ABIN499347) Images
Specificity ASK1 antibody was raised against a 16 amino acid peptide from near the carboxy terminus of human ASK1.
Purification Affinity chromatography purified via peptide column
Immunogen Human ASK1 Peptide
Isotype IgG

Target Details MAP3K5

Product Details anti-MAP3K5 Antibody Application Details Handling References for anti-MAP3K5 Antibody (ABIN499347) Images back to top
Antigen
Alternative Name MEKK5 / ASK1 (MAP3K5 Antibody Abstract)
Background Mitogen-activated protein (MAP) kinase cascades are activated in response to various extracellular stimuli, including cytokines, growth factors and environmental stresses. A novel MAP kinase kinase kinase (MAPKKK) was recently identified and designated ASK1 (for apoptosis signal-regulating kinase 1) and MAPKKK5 (1-3). ASK1 activated two different subgroups of MAPKK, MKK4 and MKK6, which in turn activated c-Jun N-terminal kinase (JNK) and p38 MAP kinase, respectively. ASK1/MAPKKK5 is activated by TNFR and Fas through the interaction with members of the TRAF family and Fas-associated protein Daxx. Overexpression of ASK1 induced apoptotic cell death, and a catalytically inactive form of ASK1 inhibited TNF-?-induced apoptosis. ASK1 is expressed in variety of tissues and cell lines.Synonyms: Apoptosis signal-regulating kinase 1, MAP3K5, MAPK/ERK kinase kinase 5, MAPKKK5, Mitogen-activated protein kinase kinase kinase 5
Gene ID 4217
UniProt Q99683
Pathways MAPK Signaling, Positive Regulation of Endopeptidase Activity

Application Details

Product Details anti-MAP3K5 Antibody Target Details MAP3K5 Handling References for anti-MAP3K5 Antibody (ABIN499347) Images back to top
Application Notes ELISA. Western Blot: ASK1 antibody can be used for the detection of ASK1 at 1 - 2 μg/mL. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-MAP3K5 Antibody Target Details MAP3K5 Application Details References for anti-MAP3K5 Antibody (ABIN499347) Images back to top
Buffer PBS containing 0.02 % Sodium Azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-MAP3K5 Antibody (ABIN499347)

Product Details anti-MAP3K5 Antibody Target Details MAP3K5 Application Details Handling Images back to top
Background publications

Ichijo, Nishida, Irie, ten Dijke, Saitoh, Moriguchi, Takagi, Matsumoto, Miyazono, Gotoh: "Induction of apoptosis by ASK1, a mammalian MAPKKK that activates SAPK/JNK and p38 signaling pathways." in: Science (New York, N.Y.), Vol. 275, Issue 5296, pp. 90-4, 1997

Wang, Diener, Jannuzzi, Trollinger, Tan, Lichenstein, Zukowski, Yao: "Molecular cloning and characterization of a novel protein kinase with a catalytic domain homologous to mitogen-activated protein kinase kinase kinase." in: The Journal of biological chemistry, Vol. 271, Issue 49, pp. 31607-11, 1997

Tobiume, Inage, Takeda, Enomoto, Miyazono, Ichijo: "Molecular cloning and characterization of the mouse apoptosis signal-regulating kinase 1." in: Biochemical and biophysical research communications, Vol. 239, Issue 3, pp. 905-10, 1997

Images

Product Details anti-MAP3K5 Antibody Target Details MAP3K5 Application Details Handling References for anti-MAP3K5 Antibody (ABIN499347) back to top
Supplier Images
Immunofluorescence (IF) image for anti-MAP3K5 antibody (Mitogen-Activated Protein Kinase Kinase Kinase 5) (ABIN499347) Immunocytochemistry of ASK1 in A431 cells with AP30086PU-N ASK1 antibody at 2 μg/ml.
Immunofluorescence (IF) image for anti-MAP3K5 antibody (Mitogen-Activated Protein Kinase Kinase Kinase 5) (ABIN499347) Immunofluorescence of ASK1 in 3T3 cells with AP30086PU-N ASK1 antibody at 20 μg/ml.
Western Blotting (WB) image for anti-MAP3K5 antibody (Mitogen-Activated Protein Kinase Kinase Kinase 5) (ABIN499347) Western blot analysis of ASK1 in SW1353 cell lysate with AP30086PU-N ASK1 antibody at...