You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Mouse (Murine) MAP3K5 antibody for Immunofluorescence

Recommended MAP3K5 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) Antibodies
  • 7420452D20Rik
  • ASK
  • ASK1
  • MAP3K5
  • MAPKKK5
  • MEKK5
  • Mekk5
  • mitogen-activated protein kinase kinase kinase 5
  • MUD21.11
  • MUD21_11
Reactivity
Human, Mouse (Murine)
374
139
78
4
1
Host
Rabbit
359
23
Clonality
Polyclonal
Conjugate
This MAP3K5 antibody is un-conjugated
13
13
12
7
7
7
5
5
5
5
5
5
5
5
5
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
287
157
136
59
55
39
23
10
8
7
6
2
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN499347
$ 401.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.32040194 ABIN189046 ICC FACS IF IHC (p) IHC WB Rabbit IgG N-Term Log in to see EP553Y
0.0010840179 ABIN197326 IF IHC (p) WB Rabbit Ser966 Log in to see Polyclonal 4
0.0010840179 ABIN362730 IF IHC Rabbit IgG AA 964-968 Log in to see Polyclonal 5
0.0010840179 ABIN2162756 IF IHC Rabbit IgG Ser966 Log in to see Polyclonal
0.0010840179 ABIN1842390 IF IHC Rabbit IgG pSer966 Log in to see Polyclonal
0.0010840179 ABIN4281869 ICC IF IHC IHC (p) WB Rabbit Log in to see Polyclonal
0.0010840179 ABIN2958733 ICC IF ELISA WB Rabbit IgG C-Term Log in to see Polyclonal
0.0010840179 ABIN1980290 IF IHC WB Rabbit Log in to see Polyclonal
0.0010840179 ABIN4281878 ELISA ICC IF IHC IHC (p) WB Rabbit C-Term Log in to see Polyclonal
0.0010840179 ABIN2968877 ICC IF IHC WB Rabbit IgG Log in to see Polyclonal
0.0010840179 ABIN273948 IF IHC Rabbit IgG Log in to see Polyclonal
0.0010840179 ABIN273949 IF IHC Rabbit IgG Log in to see Polyclonal
0.0010840179 ABIN1574853 IF IHC WB Rabbit IgG Log in to see Polyclonal

Top referenced anti-MAP3K5 antibody for Immunofluorescence

Similar anti-MAP3K5 Antibodies

Application / Reactivity Mouse (Murine) Human
ELISA 24 Antibodies 132 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies 11 Antibodies
Flow Cytometry (FACS) 1 Antibodies 8 Antibodies
Immunocytochemistry (ICC) 11 Antibodies 23 Antibodies
Immunofluorescence (IF) 14 Antibodies 41 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 44 Antibodies 55 Antibodies
Immunohistochemistry (IHC) 46 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 28 Antibodies
Western Blotting (WB) 81 Antibodies
Immunochromatography (IC) 2 Antibodies

General

Antigen Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) Antibodies
Reactivity Human, Mouse (Murine)
(374), (139), (78), (4), (1)
Host Rabbit
(359), (23)
Clonality
Polyclonal
Conjugate This MAP3K5 antibody is un-conjugated
(13), (13), (12), (7), (7), (7), (5), (5), (5), (5), (5), (5), (5), (5), (5)
Application Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
(287), (157), (136), (59), (55), (39), (23), (10), (8), (7), (6), (2), (1), (1), (1)
Pubmed 3 references available
Supplier Log in to see

Product Details anti-MAP3K5 Antibody

Target Details MAP3K5 Application Details Handling References for anti-MAP3K5 Antibody (ABIN499347) Images
Specificity ASK1 antibody was raised against a 16 amino acid peptide from near the carboxy terminus of human ASK1.
Purification Affinity chromatography purified via peptide column
Immunogen Human ASK1 Peptide
Isotype IgG

Target Details MAP3K5

Product Details anti-MAP3K5 Antibody Application Details Handling References for anti-MAP3K5 Antibody (ABIN499347) Images back to top
Antigen
Alternative Name MEKK5 / ASK1 (MAP3K5 Antibody Abstract)
Background Mitogen-activated protein (MAP) kinase cascades are activated in response to various extracellular stimuli, including cytokines, growth factors and environmental stresses. A novel MAP kinase kinase kinase (MAPKKK) was recently identified and designated ASK1 (for apoptosis signal-regulating kinase 1) and MAPKKK5 (1-3). ASK1 activated two different subgroups of MAPKK, MKK4 and MKK6, which in turn activated c-Jun N-terminal kinase (JNK) and p38 MAP kinase, respectively. ASK1/MAPKKK5 is activated by TNFR and Fas through the interaction with members of the TRAF family and Fas-associated protein Daxx. Overexpression of ASK1 induced apoptotic cell death, and a catalytically inactive form of ASK1 inhibited TNF-?-induced apoptosis. ASK1 is expressed in variety of tissues and cell lines.Synonyms: Apoptosis signal-regulating kinase 1, MAP3K5, MAPK/ERK kinase kinase 5, MAPKKK5, Mitogen-activated protein kinase kinase kinase 5
Gene ID 4217
UniProt Q99683
Pathways MAPK Signaling

Application Details

Product Details anti-MAP3K5 Antibody Target Details MAP3K5 Handling References for anti-MAP3K5 Antibody (ABIN499347) Images back to top
Application Notes ELISA. Western Blot: ASK1 antibody can be used for the detection of ASK1 at 1 - 2 μg/mL. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-MAP3K5 Antibody Target Details MAP3K5 Application Details References for anti-MAP3K5 Antibody (ABIN499347) Images back to top
Buffer PBS containing 0.02 % Sodium Azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-MAP3K5 Antibody (ABIN499347)

Product Details anti-MAP3K5 Antibody Target Details MAP3K5 Application Details Handling Images back to top
Background publications

Ichijo, Nishida, Irie, ten Dijke, Saitoh, Moriguchi, Takagi, Matsumoto, Miyazono, Gotoh: "Induction of apoptosis by ASK1, a mammalian MAPKKK that activates SAPK/JNK and p38 signaling pathways." in: Science (New York, N.Y.), Vol. 275, Issue 5296, pp. 90-4, 1997

Wang, Diener, Jannuzzi, Trollinger, Tan, Lichenstein, Zukowski, Yao: "Molecular cloning and characterization of a novel protein kinase with a catalytic domain homologous to mitogen-activated protein kinase kinase kinase." in: The Journal of biological chemistry, Vol. 271, Issue 49, pp. 31607-11, 1997

Tobiume, Inage, Takeda, Enomoto, Miyazono, Ichijo: "Molecular cloning and characterization of the mouse apoptosis signal-regulating kinase 1." in: Biochemical and biophysical research communications, Vol. 239, Issue 3, pp. 905-10, 1997

Images

Product Details anti-MAP3K5 Antibody Target Details MAP3K5 Application Details Handling References for anti-MAP3K5 Antibody (ABIN499347) back to top
Supplier Images
Immunofluorescence (IF) image for anti-MAP3K5 antibody (Mitogen-Activated Protein Kinase Kinase Kinase 5) (ABIN499347) Immunocytochemistry of ASK1 in A431 cells with AP30086PU-N ASK1 antibody at 2 μg/ml.
Immunofluorescence (IF) image for anti-MAP3K5 antibody (Mitogen-Activated Protein Kinase Kinase Kinase 5) (ABIN499347) Immunofluorescence of ASK1 in 3T3 cells with AP30086PU-N ASK1 antibody at 20 μg/ml.
Western Blotting (WB) image for anti-MAP3K5 antibody (Mitogen-Activated Protein Kinase Kinase Kinase 5) (ABIN499347) Western blot analysis of ASK1 in SW1353 cell lysate with AP30086PU-N ASK1 antibody at...