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|Application / Reactivity||Human||Mouse (Murine)||Rat (Rattus)|
|Immunofluorescence (fixed cells) (IF/ICC)||2 Antibodies||2 Antibodies||1 Antibodies|
|Western Blotting (WB)||286 Antibodies||106 Antibodies||52 Antibodies|
|Radioimmunoassay (RIA)||1 Antibodies|
|Proximity Ligation Assay (PLA)||1 Antibodies|
|Immunoprecipitation (IP)||9 Antibodies||1 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||67 Antibodies||35 Antibodies||17 Antibodies|
|Immunohistochemistry (IHC)||149 Antibodies||58 Antibodies||26 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||4 Antibodies|
|Immunohistochemistry (Acetone-fixed) (IHC (af))||1 Antibodies|
|Antigen||Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This MAP3K5 antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
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Product Details anti-MAP3K5 AntibodyTarget Details MAP3K5 Application Details Handling Images
|Specificity||This antibody detects endogenous levels of p-ASK1protein only when phosphorylated at Ser966.|
Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
|Purity||> 95 % by SDS-PAGE|
Target Details MAP3K5Product Details anti-MAP3K5 Antibody Application Details Handling Images back to top
|Alternative Name||MEKK5 / ASK1 (MAP3K5 Antibody Abstract)|
|Background||Mitogen-activated protein (MAP) kinase cascades are activated by various extracellular stimuli including growth factors. The MEK kinases (also called MAP kinase kinase kinases) phosphorylate and activate the MAP kinases, including ERK, JNK and p38. The MEK kinases characterized to date include Raf-1, Raf-B, MOS, MEK kinase-1, MEK kinase-2, MEK kinase-3, MEK kinase-4 and ASK 1 (also designated MEK kinase-5). MEK kinase-1 has been shown to phosphorylate MEK-1 via a Raf-independent pathway. Evidence suggests that MEK-3 is preferentially activated by MEK kinase-3 and that MEK-4 is activated by both MEK kinase-2 and MEK kinase-3. MEK kinase-4 has been shown to specifically activate the JNK pathway. ASK 1 activates both MEK-4 and MEK-3/MEK-6 pathways.Synonyms: Apoptosis signal-regulating kinase 1, MAP3K5, MAPK/ERK kinase kinase 5, MAPKKK5, Mitogen-activated protein kinase kinase kinase 5|
|Molecular Weight||approx. 155 kDa|
Application DetailsProduct Details anti-MAP3K5 Antibody Target Details MAP3K5 Handling Images back to top
Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-MAP3K5 Antibody Target Details MAP3K5 Application Details Images back to top
|Buffer||Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
ImagesProduct Details anti-MAP3K5 Antibody Target Details MAP3K5 Application Details Handling back to top