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|Antigen||Mitogen-Activated Protein Kinase Kinase Kinase Kinase 3 (MAP4K3) Antibodies|
|Conjugate||This MAP4K3 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|1 reference available|
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Product Details anti-MAP4K3 AntibodyTarget Details MAP4K3 Application Details Handling References for anti-MAP4K3 antibody (ABIN360428) Images
|Specificity||This antibody reacts to GLK.|
|Purification||Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS|
|Immunogen||This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human GLK.|
Target Details MAP4K3Product Details anti-MAP4K3 Antibody Application Details Handling References for anti-MAP4K3 antibody (ABIN360428) Images back to top
|Alternative Name||MAP4K3 (MAP4K3 Antibody Abstract)|
|Background||GLK is a member of the Ste20 family of serine/threonine protein kinases. The protein belongs to the subfamily that consists of members, such as germinal center kinase (GCK), that are characterized by an N-terminal catalytic domain and C-terminal regulatory domain. The kinase activity of the encoded protein can be stimulated by UV radiation and tumor necrosis factor-alpha. The protein specifically activates the c-Jun N-terminal kinase (JNK) signaling pathway. Evidence suggests that it functions upstream of mitogen-activated protein kinase kinase kinase 1 (MEKK1). This gene previously was referred to as RAB8-interacting protein-like 1 (RAB8IPL1), but it has been renamed mitogen-activated protein kinase kinase kinase kinase 3 (MAP4K3).Synonyms: Germinal center kinase-related protein kinase, MAPK, MAPK/ERK kinase kinase kinase 3, MEKKK 3, Mitogen-activated protein kinase kinase kinase kinase 3, RAB8IPL1|
|Gene ID||8491, 9606|
Application DetailsProduct Details anti-MAP4K3 Antibody Target Details MAP4K3 Handling References for anti-MAP4K3 antibody (ABIN360428) Images back to top
ELISA: 1/1,000. Western blotting: 1/250 - 1/500. Immunohistochemistry: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-MAP4K3 Antibody Target Details MAP4K3 Application Details References for anti-MAP4K3 antibody (ABIN360428) Images back to top
|Buffer||PBS with 0.09 % (W/V) sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.|
References for anti-MAP4K3 antibody (ABIN360428)Product Details anti-MAP4K3 Antibody Target Details MAP4K3 Application Details Handling Images back to top
Diener, Wang, Chen, Meyer, Keesler, Zukowski, Tan, Yao: "Activation of the c-Jun N-terminal kinase pathway by a novel protein kinase related to human germinal center kinase." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 18, pp. 9687-92, 1997
ImagesProduct Details anti-MAP4K3 Antibody Target Details MAP4K3 Application Details Handling References for anti-MAP4K3 antibody (ABIN360428) back to top