|Application / Reactivity||Mouse (Murine)||Human|
|ELISA||4 Antibodies||49 Antibodies|
|Enzyme Immunoassay (EIA)||1 Antibodies||2 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||3 Antibodies||3 Antibodies|
|Immunohistochemistry (IHC)||8 Antibodies||39 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||4 Antibodies||11 Antibodies|
|Western Blotting (WB)||19 Antibodies||90 Antibodies|
|Flow Cytometry (FACS)||8 Antibodies|
|Immunocytochemistry (ICC)||1 Antibodies|
|Immunofluorescence (IF)||16 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||1 Antibodies|
|Immunoprecipitation (IP)||5 Antibodies|
|Proximity Ligation Assay (PLA)||1 Antibodies|
|Antigen||Mitogen-Activated Protein Kinase 12 (MAPK12) Antibodies|
|Reactivity||Human, Mouse (Murine) Alternatives|
|Conjugate||This MAPK12 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|5 references available|
|Supplier||Log in to see|
Product Details anti-MAPK12 AntibodyTarget Details MAPK12 Application Details Handling References for anti-MAPK12 Antibody (ABIN359646) Images
|Specificity||This antibody reacts to p38 gamma (MAPK12).|
|Purification||Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS|
|Immunogen||This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human P38 gamma.|
Target Details MAPK12Product Details anti-MAPK12 Antibody Application Details Handling References for anti-MAPK12 Antibody (ABIN359646) Images back to top
|Alternative Name||MAPK12 (MAPK12 Antibody Abstract)|
|Background||P38 gamma is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. Activation of members of the mitogen-activated protein kinase family is a major mechanism for transduction of extracellular signals. Stress-activated protein kinases are one subclass of MAP kinases. P38 gamma functions as a signal transducer during differentiation of myoblasts to myotubes.Synonyms: ERK5, ERK6, Extracellular signal-regulated kinase 6, MAP kinase p38 gamma, Mitogen-activated protein kinase 12, SAPK3, Stress-activated protein kinase 3, p38 gamma|
|Gene ID||6300, 9606|
|Pathways||MAPK Signaling, Neurotrophin Signaling Pathway|
Application DetailsProduct Details anti-MAPK12 Antibody Target Details MAPK12 Handling References for anti-MAPK12 Antibody (ABIN359646) Images back to top
ELISA: 1/1,000. Western blotting: 1/100 - 1/500. Immunohistochemistry: 1/10 - 1/50.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-MAPK12 Antibody Target Details MAPK12 Application Details References for anti-MAPK12 Antibody (ABIN359646) Images back to top
|Buffer||PBS with 0.09 % (W/V) sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.|
References for anti-MAPK12 Antibody (ABIN359646)Product Details anti-MAPK12 Antibody Target Details MAPK12 Application Details Handling Images back to top
Julien, Coulombe, Meloche: "Nuclear export of ERK3 by a CRM1-dependent mechanism regulates its inhibitory action on cell cycle progression." in: The Journal of biological chemistry, Vol. 278, Issue 43, pp. 42615-24, 2003 (PubMed).
García-Lora, Martinez, Pedrinaci et al.: "Different regulation of PKC isoenzymes and MAPK by PSK and IL-2 in the proliferative and cytotoxic activities of the NKL human natural killer cell line." in: Cancer immunology, immunotherapy : CII, Vol. 52, Issue 1, pp. 59-64, 2003 (PubMed).
Court, dos Remedios, Cordell et al.: "Cardiac expression and subcellular localization of the p38 mitogen-activated protein kinase member, stress-activated protein kinase-3 (SAPK3)." in: Journal of molecular and cellular cardiology, Vol. 34, Issue 4, pp. 413-26, 2002 (PubMed).
Robinson, Xu Be, Stippec et al.: "Different domains of the mitogen-activated protein kinases ERK3 and ERK2 direct subcellular localization and upstream specificity in vivo." in: The Journal of biological chemistry, Vol. 277, Issue 7, pp. 5094-100, 2002 (PubMed).
Wang, McGowan, Zhao et al.: "Involvement of the MKK6-p38gamma cascade in gamma-radiation-induced cell cycle arrest." in: Molecular and cellular biology, Vol. 20, Issue 13, pp. 4543-52, 2000 (PubMed).
ImagesProduct Details anti-MAPK12 Antibody Target Details MAPK12 Application Details Handling References for anti-MAPK12 Antibody (ABIN359646) back to top