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anti-Guinea Pig MAPK14 antibody for Western Blotting

Recommended MAPK14 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase 14 (MAPK14) Antibodies
  • 186F5S
  • anon-sts23
  • AP22.98
  • AP22_98
  • ATMPK14
  • BG:DS00797.3
  • CG7393
  • CRK1
  • Crk1
  • CSBP
  • csbp
  • Csbp1
  • CSBP1
  • Csbp2
  • CSBP2
  • CSPB1
  • D-p38
  • D-p38 MAPK
  • D-p38b
  • Dmel\\CG7393
  • Dmp38b
  • Dm p38b
  • Dp38
  • dp38b
  • Dp38b
  • ESTS:186F5S
  • Exip
  • EXIP
  • Hog
  • mapk14a
  • mitogen-activated protein kinase 14
  • Mpk34C
  • Mxi2
  • mxi2
  • p38
  • p38 beta
  • p38 MAPK
  • p38-alpha
  • p38a
  • p38alpha
  • p38ALPHA
  • p38B
  • p38beta
  • p38Hog
  • p38Kb
  • p38MAPK
  • Prkm14
  • PRKM14
  • Prkm15
  • PRKM15
  • RK
  • sapk2
  • sapk2a
  • SAPK2A
  • Sapk2A
  • SAPK2a
Epitope
AA 341-360
29
25
17
13
12
11
10
9
8
7
7
7
5
5
4
4
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Chicken, Guinea Pig, Hamster, Human, Monkey, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Sheep (Ovine)
178
80
72
17
7
6
5
4
3
1
1
1
1
1
1
1
Host
Rabbit
151
30
2
1
Clonality
Polyclonal
Conjugate
This MAPK14 antibody is un-conjugated
7
7
6
5
5
5
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB)
163
77
53
28
22
10
10
8
7
7
4
2
1
Supplier
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Catalog No. ABIN264893
$ 308.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0011728023 ABIN302107 IP WB Rabbit AA 341-360 Log in to see Polyclonal

Top referenced anti-MAPK14 antibody for Western Blotting

Similar anti-MAPK14 Antibodies

Application / Reactivity Dog (Canine) Human Mouse (Murine) Rat (Rattus) Cow (Bovine) Chicken Guinea Pig Hamster Monkey Pig (Porcine) Rabbit Sheep (Ovine)
Western Blotting (WB) 18 Antibodies 161 Antibodies 76 Antibodies 70 Antibodies 8 Antibodies 6 Antibodies 2 Antibodies 2 Antibodies 7 Antibodies 2 Antibodies 2 Antibodies 2 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Immunoprecipitation (IP) 4 Antibodies

General

Antigen Mitogen-Activated Protein Kinase 14 (MAPK14) Antibodies
Epitope AA 341-360
(29), (25), (17), (13), (12), (11), (10), (9), (8), (7), (7), (7), (5), (5), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Chicken, Guinea Pig, Hamster, Human, Monkey, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Sheep (Ovine)
(178), (80), (72), (17), (7), (6), (5), (4), (3), (1), (1), (1), (1), (1), (1), (1)
Host Rabbit
(151), (30), (2), (1)
Clonality
Polyclonal
Conjugate This MAPK14 antibody is un-conjugated
(7), (7), (6), (5), (5), (5)
Application Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB)
(163), (77), (53), (28), (22), (10), (10), (8), (7), (7), (4), (2), (1)
Pubmed 7 references available
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Product Details anti-MAPK14 Antibody

Target Details MAPK14 Application Details Handling References for anti-MAPK14 Antibody (ABIN264893) Images
Specificity Detects ~43 kDa corresponding to the molecular mass of p38 on SDS-PAGE immunoblots.
Purification Serum
Immunogen A 20 residue synthetic peptide based on the Human p38 (residues 341-360) with the Cysteine residue added and coupled to KLH
Isotype IgG

Target Details MAPK14

Product Details anti-MAPK14 Antibody Application Details Handling References for anti-MAPK14 Antibody (ABIN264893) Images back to top
Antigen
Alternative Name MAP Kinase p38 alpha / MAPK14 (MAPK14 Antibody Abstract)
Background The MAPK (mitogen activated protein kinase) comprises a family of ubiquitous praline-directed, protein-serine/threonine kinases which signal transduction pathways that control intracellular events including acute responses to hormones and major developmental changes in organisms (1). This super family consists of stress activated protein kinases (SAPKs), extracellular signal-regulated kinases (ERKs), and p38 kinases, each of which forms a separate pathway (2). The kinase members that populate each pathway are sequentially activated by phosphorylation. Upon activation, p38 MAPK/SAPK2α translocates into the nucleus where it phosphorylates one or more nuclear substrates, effecting transcriptional changes and other cellular processes involved in cell growth, division, differentiation, inflammation, and death (3). Specifically p38 always acts as a pro-apoptotic factor with its activation leading to the release of cytochrome c from mitochondria and cleavage of caspase 3 and its downstream effector, PARP (4). p38 MAPK is activated by a variety of chemical stress inducers including hydrogen peroxide, heavy metals, anisomycin, sodium salicylate, LPS, and biological stress signals such as tumor necrosis factor, interleukin-1, ionizing and UV irradiation, hyperosmotic stress and chemotherapeutic drugs (5). As a result, p38 alpha has been widely validated as a target for inflammatory disease including rheumatoid arthritis, COPD and psoriasis (6) and has also been implicated in cancer, CNS and diabetes (7).Synonyms: CSBP, CSBP1, CSBP2, CSPB1, Cytokine suppressive anti-inflammatory drug-binding protein, MAX-interacting protein 2, MXI2, Mitogen-activated protein kinase 14, SAPK2A, p38 alpha
Gene ID 1432
NCBI Accession NP_001306
UniProt Q16539
Research Area Kinases/Phosphatases
Pathways MAPK Signaling, TCR Signaling, Neurotrophin Signaling Pathway

Application Details

Product Details anti-MAPK14 Antibody Target Details MAPK14 Handling References for anti-MAPK14 Antibody (ABIN264893) Images back to top
Application Notes Western blot: A 1/5000 dilution of this antibody was sufficient for detection of p38 in 20 μgof HeLa cell lysate by ECL Immunoblot analysis. Immunoprecipitation: 1/250. Immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-MAPK14 Antibody Target Details MAPK14 Application Details References for anti-MAPK14 Antibody (ABIN264893) Images back to top
Format Liquid
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-MAPK14 Antibody (ABIN264893)

Product Details anti-MAPK14 Antibody Target Details MAPK14 Application Details Handling Images back to top
Background publications

Fan, Chen, Qiao, Luo, Ma, Li, Jiang, Niu, Yin: "Opposing effects of ERK and p38 MAP kinases on HeLa cell apoptosis induced by dipyrithione." in: Molecules and cells, Vol. 23, Issue 1, pp. 30-8, 2007

Salojin, Owusu, Millerchip, Potter, Platt, Oravecz: "Essential role of MAPK phosphatase-1 in the negative control of innate immune responses." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 176, Issue 3, pp. 1899-907, 2006

Medicherla, Protter, Ma, Mangadu, Almirez, Koppelman, Kerr, Navas, Movius, Reddy, Liu, Luedtke, Perumattam, Mavunkel, Dugar, Schreiner: "Preventive and therapeutic potential of p38 alpha-selective mitogen-activated protein kinase inhibitor in nonobese diabetic mice with type 1 diabetes." in: The Journal of pharmacology and experimental therapeutics, Vol. 318, Issue 1, pp. 99-107, 2006

Van Laethem, Van Kelst, Lippens, Declercq, Vandenabeele, Janssens, Vandenheede, Garmyn, Agostinis: "Activation of p38 MAPK is required for Bax translocation to mitochondria, cytochrome c release and apoptosis induced by UVB irradiation in human keratinocytes." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 18, Issue 15, pp. 1946-8, 2004

Deng, Ren, Yang, Lin, Wu: "A JNK-dependent pathway is required for TNFalpha-induced apoptosis." in: Cell, Vol. 115, Issue 1, pp. 61-70, 2003

Pearson, Robinson, Beers Gibson, Xu, Karandikar, Berman, Cobb: "Mitogen-activated protein (MAP) kinase pathways: regulation and physiological functions." in: Endocrine reviews, Vol. 22, Issue 2, pp. 153-83, 2001

Han, Lee, Bibbs, Ulevitch: "A MAP kinase targeted by endotoxin and hyperosmolarity in mammalian cells." in: Science (New York, N.Y.), Vol. 265, Issue 5173, pp. 808-11, 1994

Images

Product Details anti-MAPK14 Antibody Target Details MAPK14 Application Details Handling References for anti-MAPK14 Antibody (ABIN264893) back to top
Supplier Images
 image for anti-MAPK14 antibody (Mitogen-Activated Protein Kinase 14) (AA 341-360) (ABIN264893) anti-Mitogen-Activated Protein Kinase 14 (MAPK14) (AA 341-360) antibody