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|Application / Reactivity||Human||Mouse (Murine)||Rat (Rattus)||Dog (Canine)|
|Immunofluorescence (fixed cells) (IF/ICC)||8 Antibodies||4 Antibodies||4 Antibodies|
|Western Blotting (WB)||18 Antibodies||164 Antibodies||75 Antibodies||67 Antibodies|
|Proximity Ligation Assay (PLA)||2 Antibodies|
|Immunoprecipitation (IP)||4 Antibodies||22 Antibodies||11 Antibodies||12 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||11 Antibodies||7 Antibodies|
|Antigen||Mitogen-Activated Protein Kinase 14 (MAPK14) Antibodies|
|Reactivity||Dog (Canine), Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This MAPK14 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB)
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Product Details anti-MAPK14 AntibodyTarget Details MAPK14 Application Details Handling Images
|Specificity||This antibody is directed against the N-terminus of Mxi2.|
|Characteristics||Synonyms: Mitogen-activated protein kinase 14, p38 alpha, MXI2, SAPK2A, CSBP, CSBP1, CSBP2,CSPB1, Cytokine suppressive anti-inflammatory drug-binding protein, MAX-interactingprotein 2|
|Purification||Size exclusion chromatography|
|Components||incl. positive Control|
|Immunogen||Synthetic peptide conjugated to KLH|
Target Details MAPK14Product Details anti-MAPK14 Antibody Application Details Handling Images back to top
|Alternative Name||MAP Kinase p38 alpha / MAPK14 (MAPK14 Antibody Abstract)|
|Background||Mxi2 is a splice variant of SAPK2α (p38). Stress-activated protein kinases (SAPK) are strongly activated in response to adverse stimuli such as heat and osmotic shock, UV light and other DNA-damaging reagents, and inhibitors of protein synthesis. They are also activated strongly in response to agonists that are released or produced under conditions of stress, such as proinflammatory cytokines.Synonyms: CSBP, CSBP1, CSBP2, CSPB1, Cytokine suppressive anti-inflammatory drug-binding protein, MAX-interacting protein 2, MXI2, Mitogen-activated protein kinase 14, SAPK2A, p38 alpha|
|Pathways||MAPK Signaling, TCR Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Muscle Cell Differentiation|
Application DetailsProduct Details anti-MAPK14 Antibody Target Details MAPK14 Handling Images back to top
Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. Positive Control: Cell lysate from untreated A431 cells. ELISA: 0.05 g/m. Immunoprecipitation: 1 - 10 g per 10^6 pervanadate-treated A431 cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-MAPK14 Antibody Target Details MAPK14 Application Details Images back to top
|Reconstitution||Restore with 1 mL H2O (15 min, RT).|
|Buffer||1 mL2 x PBS / 0.09 % Na-azide / PEG and Sucrose|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage||-20 °C/-80 °C|
Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch.
|Expiry Date||12 months|
ImagesProduct Details anti-MAPK14 Antibody Target Details MAPK14 Application Details Handling back to top