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anti-Rat (Rattus) MAPK14 antibody for Immunofluorescence

Recommended MAPK14 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase 14 (MAPK14) Antibodies
  • 186F5S
  • anon-sts23
  • AP22.98
  • AP22_98
  • ATMPK14
  • BG:DS00797.3
  • CG7393
  • CRK1
  • Crk1
  • CSBP
  • csbp
  • Csbp1
  • CSBP1
  • Csbp2
  • CSBP2
  • CSPB1
  • D-p38
  • D-p38 MAPK
  • D-p38b
  • Dmel\\CG7393
  • Dmp38b
  • Dm p38b
  • Dp38
  • dp38b
  • Dp38b
  • ESTS:186F5S
  • Exip
  • EXIP
  • Hog
  • mapk14a
  • mitogen-activated protein kinase 14
  • Mpk34C
  • Mxi2
  • mxi2
  • p38
  • p38 beta
  • p38 MAPK
  • p38-alpha
  • p38a
  • p38alpha
  • p38ALPHA
  • p38B
  • p38beta
  • p38Hog
  • p38Kb
  • p38MAPK
  • Prkm14
  • PRKM14
  • Prkm15
  • PRKM15
  • RK
  • sapk2
  • sapk2a
  • SAPK2A
  • Sapk2A
  • SAPK2a
Epitope
AA 243-355
29
25
17
13
12
11
10
9
8
7
7
7
5
5
4
4
4
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
178
80
72
17
8
7
6
4
3
2
2
2
2
2
1
1
Host
Mouse
152
29
2
1
Clonality
Monoclonal
Conjugate
This MAPK14 antibody is un-conjugated
7
7
6
5
5
5
Application
Immunofluorescence (IF), Western Blotting (WB)
163
77
53
27
23
10
10
8
7
7
5
2
1
Supplier
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Catalog No. ABIN968769
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.31611317 ABIN968770 IF WB Mouse IgG1 AA 243-355 Log in to see 27-p38alpha-SAPK2a 4
0.31611317 ABIN2448648 IF IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.31611317 ABIN2406906 IF WB Rabbit IgG pThr180 Log in to see Polyclonal
0.31611317 ABIN2406908 IF WB Rabbit IgG pTyr182 Log in to see Polyclonal
0.31611317 ABIN2403460 IF IHC WB Rabbit IgG Log in to see Polyclonal
0.31611317 ABIN2406904 IF WB Rabbit IgG pTyr322 Log in to see Polyclonal
0.0010850475 ABIN126883 EIA IF IP WB Mouse IgG1 N-Term Log in to see 13D5
0.0010850475 ABIN498575 IF IHC (p) WB Rabbit Log in to see Polyclonal
0.0010850475 ABIN2563762 IF IHC WB Rabbit IgG Log in to see Polyclonal
0.0010850475 ABIN2987256 ICC IF WB Rabbit IgG pTyr322 Log in to see Polyclonal
0.0010850475 ABIN2987259 ICC IF WB Rabbit IgG pThr180 Log in to see Polyclonal
0.0010850475 ABIN2987262 ICC IF WB Rabbit IgG pTyr182 Log in to see Polyclonal
0.0010850475 ABIN2988478 ICC IF IHC WB Rabbit IgG Log in to see Polyclonal

Top referenced anti-MAPK14 antibody for Immunofluorescence

Similar anti-MAPK14 Antibodies

Application / Reactivity Dog (Canine) Human Mouse (Murine) Rat (Rattus)
Western Blotting (WB) 18 Antibodies 161 Antibodies 76 Antibodies 70 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Immunoprecipitation (IP) 4 Antibodies 22 Antibodies 12 Antibodies 14 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 12 Antibodies 7 Antibodies 5 Antibodies
Immunohistochemistry (IHC) 24 Antibodies

General

Antigen Mitogen-Activated Protein Kinase 14 (MAPK14) Antibodies
Epitope AA 243-355
(29), (25), (17), (13), (12), (11), (10), (9), (8), (7), (7), (7), (5), (5), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
(178), (80), (72), (17), (8), (7), (6), (4), (3), (2), (2), (2), (2), (2), (1), (1)
Host Mouse
(152), (29), (2), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This MAPK14 antibody is un-conjugated
(7), (7), (6), (5), (5), (5)
Application Immunofluorescence (IF), Western Blotting (WB)
(163), (77), (53), (27), (23), (10), (10), (8), (7), (7), (5), (2), (1)
Pubmed 4 references available
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Product Details anti-MAPK14 Antibody

Target Details MAPK14 Application Details Handling References for anti-MAPK14 Antibody (ABIN968769) Images
Cross-Reactivity Dog (Canine), Mouse (Murine), Rat (Rattus)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Human p38 MAPK aa. 243-355
Clone 27-p38alpha-SAPK2a
Isotype IgG1

Target Details MAPK14

Product Details anti-MAPK14 Antibody Application Details Handling References for anti-MAPK14 Antibody (ABIN968769) Images back to top
Antigen
Alternative Name p38alpha (MAPK14 Antibody Abstract)
Background Activation of the immune and inflammatory responses often involves the recognition of bacterial endotoxin (lipopolysaccharide or LPS). Binding of LPS by monocytic cells results in the production and release of proinflammatory cytokines, such as IL-1 and TNF-alpha. LPS-induced signaling cascades involve members of the Ser/Thr protein kinase family known as the mitogen activated protein kinases (MAPKs). One of these, p38 MAP kinase (p38 MAPK or CSPB2), is a human homologue of HOG1, a yeast MAP kinase that is essential for growth under conditions of elevated osmolarity. Similar to HOG1, p38 MAPK is phosphorylated in response to hyperosmolarity, but also in response to a variety of stimuli including LPS, IL-1, or TNF-alpha. Efficient activation of p38 MAPK requires phosphorylation of Thr-180 and Tyr-182. At least three Thr/Tyr kinases (MKK3, MKK4/SEK1, and MKK6) phosphorylate and activate p38 MAPK. This leads to the activation of multiple transcription factors (NF-kappaB, ATF-2, Elk-1, and CHOP/GADD143) that induce expression of proinflammatory cytokine genes. In addition to its role in immunity, p38 MAPK is widely expressed and serves as an important signal transducer in a variety of cell types. This antibody is routinely tested by western blot analysis.
Synonyms: SAPK2a
Molecular Weight 42 kDa
Pathways MAPK Signaling, TCR Signaling, Neurotrophin Signaling Pathway

Application Details

Product Details anti-MAPK14 Antibody Target Details MAPK14 Handling References for anti-MAPK14 Antibody (ABIN968769) Images back to top
Comment

Related Products: ABIN968537, ABIN967389

Restrictions For Research Use only

Handling

Product Details anti-MAPK14 Antibody Target Details MAPK14 Application Details References for anti-MAPK14 Antibody (ABIN968769) Images back to top
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20° C.

References for anti-MAPK14 Antibody (ABIN968769)

Product Details anti-MAPK14 Antibody Target Details MAPK14 Application Details Handling Images back to top
Product cited in:

Morinobu, Gadina, Strober, Visconti, Fornace, Montagna, Feldman, Nishikomori, OShea: "STAT4 serine phosphorylation is critical for IL-12-induced IFN-gamma production but not for cell proliferation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 19, pp. 12281-6, 2002

Winston, Chan, Johnson, Riches: "Activation of p38mapk, MKK3, and MKK4 by TNF-alpha in mouse bone marrow-derived macrophages." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 159, Issue 9, pp. 4491-7, 1997

Brunet, Pouysségur: "Identification of MAP kinase domains by redirecting stress signals into growth factor responses." in: Science (New York, N.Y.), Vol. 272, Issue 5268, pp. 1652-5, 1996

Han, Lee, Bibbs, Ulevitch: "A MAP kinase targeted by endotoxin and hyperosmolarity in mammalian cells." in: Science (New York, N.Y.), Vol. 265, Issue 5173, pp. 808-11, 1994

Images

Product Details anti-MAPK14 Antibody Target Details MAPK14 Application Details Handling References for anti-MAPK14 Antibody (ABIN968769) back to top
Supplier Images
Western Blotting (WB) image for anti-MAPK14 antibody (Mitogen-Activated Protein Kinase 14) (AA 243-355) (ABIN968769) Western blot analysis of p38alpha on a Jurkat lysate. Lane 1: 1:5000, lane 2: 1:10000...
Immunofluorescence (IF) image for anti-MAPK14 antibody (Mitogen-Activated Protein Kinase 14) (AA 243-355) (ABIN968769) Immunofluorescence staining of HeLa cells.