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|Antigen||Mitogen-Activated Protein Kinase 14 (MAPK14) Antibodies|
|Epitope||pThr180, pTyr182 Alternatives|
|Reactivity||Cow (Bovine), Dog (Canine), Chicken, Human, Monkey, Mouse (Murine), Rat (Rattus), Zebrafish (Danio rerio) Alternatives|
|Conjugate||This MAPK14 antibody is un-conjugated Alternatives|
Western Blotting (WB)
|1 reference available|
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Product Details anti-MAPK14 AntibodyTarget Details MAPK14 Application Details Handling References for anti-MAPK14 antibody (ABIN319104) Images
|Specificity||This antibody is specific for p38 mitogen-activated protein kinase (p38 MAPK), also known as MAPK14, when phosphorylated at Threonine 180 and Tyrosine 182.|
Species reactivity (expected):Chicken, Zebrafish, Bovine, Monkey, Mouse and Canine (Dog).
Species reactivity (tested):Human and Rat.
|Immunogen||Synthetic phosphopeptide corresponding to an amino acid sequence within p38 MAPK which includes phosphorylated Thr180 and Tyr182.|
Target Details MAPK14Product Details anti-MAPK14 Antibody Application Details Handling References for anti-MAPK14 antibody (ABIN319104) Images back to top
|Alternative Name||MAP Kinase p38 alpha / MAPK14 (MAPK14 Antibody Abstract)|
|Background||P38 MAPK is a serine/threonine kinase which plays an important role in signal transduction, contributing to the regulation of many cellular processes including cell differentiation and inflammation. The protein is activated by phosphorylation of threonine 180 and tyrosine 182, by several upstream kinases, in response to a wide range of extracellular stimuli such as UV B irradiation or endotoxin exposure.Synonyms: CSBP, CSBP1, CSBP2, CSPB1, Cytokine suppressive anti-inflammatory drug-binding protein, MAX-interacting protein 2, MXI2, Mitogen-activated protein kinase 14, SAPK2A, p38 alpha|
|Pathways||MAPK Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Hepatitis C, Toll-Like Receptors Cascades, Autophagy, Thromboxane A2 Receptor Signaling|
Application DetailsProduct Details anti-MAPK14 Antibody Target Details MAPK14 Handling References for anti-MAPK14 antibody (ABIN319104) Images back to top
Western Blot: 1/1000. This p38 MAPK antibody Recognises a band of approximately 39 kD in Western blots ofanisomycin C-6 glioma cell lysates. For the detection of phosphoproteins, Threonine and Tyrosine phosphatase inhibitors suchas 10 mM Sodium Fluoride and 1 mM Sodium Orthovanadate should be added to the samplebuffer. Milk or other casein-based blocking solutions are not recommended as casein is aphosphoprotein and its use can result in high background.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-MAPK14 Antibody Target Details MAPK14 Application Details References for anti-MAPK14 antibody (ABIN319104) Images back to top
|Buffer||10 mM HEPES, pH 7.5 containing 0.09 % Sodium Azide, 0.01 % BSA and 50 % Glycerol.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
References for anti-MAPK14 antibody (ABIN319104)Product Details anti-MAPK14 Antibody Target Details MAPK14 Application Details Handling Images back to top
Raingeaud, Gupta, Rogers, Dickens, Han, Ulevitch, Davis: "Pro-inflammatory cytokines and environmental stress cause p38 mitogen-activated protein kinase activation by dual phosphorylation on tyrosine and threonine." in: The Journal of biological chemistry, Vol. 270, Issue 13, pp. 7420-6, 1995