anti-Zebrafish (Danio rerio) MAPK14 antibody for Western Blotting

Recommended MAPK14 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase 14 (MAPK14) Antibodies
  • AP22.98
  • AP22_98
  • ATMPK14
  • mitogen-activated protein kinase 14
  • 186F5S
  • BG:DS00797.3
  • CG7393
  • D-p38
  • D-p38 MAPK
  • D-p38b
  • Dm p38b
  • Dmel\\CG7393
  • Dmp38b
  • Dp38
  • Dp38b
  • ESTS:186F5S
  • Mpk34C
  • anon-sts23
  • dp38b
  • p38
  • p38 MAPK
  • p38 beta
  • p38B
  • p38Kb
  • p38beta
  • CSBP
  • CSBP1
  • CSBP2
  • CSPB1
  • EXIP
  • Mxi2
  • PRKM14
  • PRKM15
  • RK
  • SAPK2A
  • p38ALPHA
  • Crk1
  • Csbp1
  • p38-alpha
  • p38MAPK
  • p38a
  • p38alpha
  • SAPK2a
  • CRK1
  • Csbp2
  • Exip
  • Hog
  • Prkm14
  • Prkm15
  • Sapk2A
  • p38Hog
  • csbp
  • mapk14a
  • mxi2
  • sapk2
  • sapk2a
  • mitogen-activated protein kinase 14
  • CG7393 gene product from transcript CG7393-RA
  • mitogen activated protein kinase 14
  • MPK14
  • MAPK14
  • Mapk14
  • p38b
  • mapk14
Epitope
pThr180, pTyr182
50
42
24
17
16
10
10
9
9
9
8
8
8
8
7
5
5
5
5
4
4
4
4
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Chicken, Human, Monkey, Mouse (Murine), Rat (Rattus), Zebrafish (Danio rerio)
298
135
128
25
16
15
12
11
11
10
7
5
5
3
3
3
3
2
1
1
1
Host
Rabbit
240
60
2
2
Clonality
Polyclonal
Conjugate
This MAPK14 antibody is un-conjugated
12
9
9
9
9
7
1
1
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
277
126
74
57
38
38
13
9
9
7
6
3
2
1
1
Supplier
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Catalog No. ABIN319104
$ 665.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN5551610 IHC (fro) WB Rabbit IgG pThr180, pTyr182 Log in to see Polyclonal
1 ABIN319100 WB Rabbit IgG pThr202, pTyr204 Log in to see Polyclonal
1 ABIN2959838 IHC WB Rabbit IgG pThr180, pTyr182 Log in to see Polyclonal

General

Antigen Mitogen-Activated Protein Kinase 14 (MAPK14) Antibodies
Epitope pThr180, pTyr182
(50), (42), (24), (17), (16), (10), (10), (9), (9), (9), (8), (8), (8), (8), (7), (5), (5), (5), (5), (4), (4), (4), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Chicken, Human, Monkey, Mouse (Murine), Rat (Rattus), Zebrafish (Danio rerio)
(298), (135), (128), (25), (16), (15), (12), (11), (11), (10), (7), (5), (5), (3), (3), (3), (3), (2), (1), (1), (1)
Host Rabbit
(240), (60), (2), (2)
Clonality
Polyclonal
Conjugate This MAPK14 antibody is un-conjugated
(12), (9), (9), (9), (9), (7), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Western Blotting (WB)
(277), (126), (74), (57), (38), (38), (13), (9), (9), (7), (6), (3), (2), (1), (1)
Pubmed 1 reference available
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Product Details anti-MAPK14 Antibody

Target Details MAPK14 Application Details Handling References for anti-MAPK14 antibody (ABIN319104) Images
Specificity This antibody is specific for p38 mitogen-activated protein kinase (p38 MAPK), also known as MAPK14, when phosphorylated at Threonine 180 and Tyrosine 182.
Cross-Reactivity (Details) Species reactivity (expected):Chicken, Zebrafish, Bovine, Monkey, Mouse and Canine (Dog).
Species reactivity (tested):Human and Rat.
Purification Affinity Chromatography.
Immunogen Synthetic phosphopeptide corresponding to an amino acid sequence within p38 MAPK which includes phosphorylated Thr180 and Tyr182.
Isotype IgG

Target Details MAPK14

Product Details anti-MAPK14 Antibody Application Details Handling References for anti-MAPK14 antibody (ABIN319104) Images back to top
Antigen
Alternative Name MAP Kinase p38 alpha / MAPK14 (MAPK14 Antibody Abstract)
Background P38 MAPK is a serine/threonine kinase which plays an important role in signal transduction, contributing to the regulation of many cellular processes including cell differentiation and inflammation. The protein is activated by phosphorylation of threonine 180 and tyrosine 182, by several upstream kinases, in response to a wide range of extracellular stimuli such as UV B irradiation or endotoxin exposure.Synonyms: CSBP, CSBP1, CSBP2, CSPB1, Cytokine suppressive anti-inflammatory drug-binding protein, MAX-interacting protein 2, MXI2, Mitogen-activated protein kinase 14, SAPK2A, p38 alpha
Gene ID 1432
NCBI Accession NP_001306
UniProt Q16539
Research Area Kinases/Phosphatases
Pathways MAPK Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Hepatitis C, Toll-Like Receptors Cascades, Autophagy, Thromboxane A2 Receptor Signaling

Application Details

Product Details anti-MAPK14 Antibody Target Details MAPK14 Handling References for anti-MAPK14 antibody (ABIN319104) Images back to top
Application Notes Western Blot: 1/1000. This p38 MAPK antibody Recognises a band of approximately 39 kD in Western blots ofanisomycin C-6 glioma cell lysates. For the detection of phosphoproteins, Threonine and Tyrosine phosphatase inhibitors suchas 10 mM Sodium Fluoride and 1 mM Sodium Orthovanadate should be added to the samplebuffer. Milk or other casein-based blocking solutions are not recommended as casein is aphosphoprotein and its use can result in high background.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-MAPK14 Antibody Target Details MAPK14 Application Details References for anti-MAPK14 antibody (ABIN319104) Images back to top
Format Liquid
Buffer 10 mM HEPES, pH 7.5 containing 0.09 % Sodium Azide, 0.01 % BSA and 50 % Glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-MAPK14 antibody (ABIN319104)

Product Details anti-MAPK14 Antibody Target Details MAPK14 Application Details Handling Images back to top
Background publications

Raingeaud, Gupta, Rogers, Dickens, Han, Ulevitch, Davis: "Pro-inflammatory cytokines and environmental stress cause p38 mitogen-activated protein kinase activation by dual phosphorylation on tyrosine and threonine." in: The Journal of biological chemistry, Vol. 270, Issue 13, pp. 7420-6, 1995