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|Application / Reactivity||Human||Mouse (Murine)||Rat (Rattus)||Dog (Canine)||Chicken||Cow (Bovine)||Xenopus laevis||Zebrafish (Danio rerio)||Non-Human Primate|
|Immunofluorescence (fixed cells) (IF/ICC)||7 Antibodies||5 Antibodies||5 Antibodies|
|In Vivo Imaging (IVI)||1 Antibodies|
|Western Blotting (WB)||16 Antibodies||376 Antibodies||198 Antibodies||132 Antibodies||26 Antibodies||29 Antibodies||23 Antibodies||1 Antibodies||1 Antibodies|
|Proximity Ligation Assay (PLA)||2 Antibodies||1 Antibodies|
|Immunoprecipitation (IP)||7 Antibodies||51 Antibodies||31 Antibodies||31 Antibodies|
|Antigen||Mitogen-Activated Protein Kinase 3 (MAPK3) Antibodies|
|Epitope||Tyr204, pThr202 Alternatives|
|Reactivity||Chicken, Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Non-Human Primate, Rat (Rattus), Xenopus laevis, Zebrafish (Danio rerio) Alternatives|
|Conjugate||This MAPK3 antibody is un-conjugated Alternatives|
Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
|Supplier||Log in to see|
Product Details anti-MAPK3 AntibodyTarget Details MAPK3 Application Details Handling Images
|Specificity||Recognizes the ~42k-44k ERK/MAPK phosphorylated at Thr202 and Tyr204. Immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide. The immunolabeling is completely eliminated by lambda-phosphatase.|
|Cross-Reactivity (Details)||Species reactivity (expected):Bovine, Canine, Chicken, Mouse, non-Human Primates, Xenopus and ZebrafishSpecies reactivity (tested):Human and Rat.|
|Purification||Sequential Chromatography on phospho- and dephosphopeptide affinity columns.|
|Immunogen||Phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr202 and Tyr204 of rat ERK/MAPK.|
Target Details MAPK3Product Details anti-MAPK3 Antibody Application Details Handling Images back to top
|Alternative Name||MAPK3 / ERK1 (MAPK3 Antibody Abstract)|
|Background||Extracellular-Signal Regulated Kinase/Mitogen-Activated Protein Kinase (ERK/MAPK) is an integral component of cellular signaling during mitogenesis and differentiation of mitotic cells and also is thought to play a key role in learning and memory (Adams and Sweatt, 2002, Ahn, 1993, Tanoue and Nishida, 2003, Johnson and Lapadat, 2002). The activity of this kinase is regulated by dual phosphorylation at Thr202 and Tyr204 (Ahn, 1993).Synonyms: ERK-1, ERT2, Extracellular signal-regulated kinase 1, Insulin-stimulated MAP2 kinase, MAP kinase 3, MAP kinase isoform p44, MAPK 3, Microtubule-associated protein 2 kinase, Mitogen-activated protein kinase 1, Mitogen-activated protein kinase 3, PRKM3, p44-ERK1, p44-MAPK|
|Research Area||Inflammation, Signaling|
|Pathways||MAPK Signaling, RTK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals|
Application DetailsProduct Details anti-MAPK3 Antibody Target Details MAPK3 Handling Images back to top
Western Blot: 1/1000. Immunohistochemistry: 1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-MAPK3 Antibody Target Details MAPK3 Application Details Images back to top
|Buffer||10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage Comment||Store the antibody undiluted (in aliquots) at-20 °C.|